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3.1 Influence of technical settings on CASA motility parameters of frozen-thawed stallion semen Adapted from: Hoogewijs M., Govaere J., Rijsselaere T., De Schauwer C., Vanhaesebrouck E., de Kruif A., De Vliegher S. 2009 Proceedings of the 55 th annual convention of the American Association of Equine Practitioners – Las Vegas - USA 55:336-337. 77
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
Page 33 and 34: CHAPTER 1.1 The sperm chromatin str
Page 35: Collection and processing of equine
Page 38 and 39: CHAPTER 1.2 30 (a) Colorado State U
Page 40 and 41: CHAPTER 1.2 can be induced pharmaco
Page 42 and 43: CHAPTER 1.2 centrifugation time and
Page 44 and 45: CHAPTER 1.2 36 → Colloid centrifu
Page 46 and 47: CHAPTER 1.2 of a stallion stored fo
Page 48 and 49: CHAPTER 1.2 1.2.4. Cryopreservation
Page 50 and 51: CHAPTER 1.2 0.5 mL straws, and glyc
Page 52 and 53: CHAPTER 1.2 determined if the final
Page 54 and 55: CHAPTER 1.2 46 Chemically defined e
Page 56 and 57: CHAPTER 1.2 48 Cryopreservation - t
Page 59 and 60: 1.3.1. Standards for equine AI dose
Page 61 and 62: CHAPTER 1.3 The use of density grad
Page 63 and 64: CHAPTER 1 Avery B., Greve T. 1995.
Page 65 and 66: CHAPTER 1 Cheng F.-P., Fazeli A., V
Page 67 and 68: CHAPTER 1 Flipse R.J., Patton S., A
Page 69 and 70: CHAPTER 1 Kuisma P., Andersson M.,
Page 71 and 72: CHAPTER 1 Medeiros A.S.L., Gomes G.
Page 73 and 74: CHAPTER 1 Pickett B.W. 1993. Collec
Page 75 and 76: CHAPTER 1 Taberner E., Morató R.,
Page 77: CHAPTER 1 Waite J.A., Love C.C., Br
Page 81: CHAPTER 2 As evidenced in the gener
Page 87 and 88: 3.1.1. Abstract CHAPTER 3.1 The rep
Page 89 and 90: 3.1.4. Results CHAPTER 3.1 Analyzin
Page 91 and 92: Totoal Motility CASA2 (%) 100 80 60
Page 93: 3.2 Counting chamber type influence
Page 96 and 97: CHAPTER 3.2 88 In addition, it has
Page 98 and 99: CHAPTER 3.2 90 3.2.3.2. Quality ana
Page 100 and 101: CHAPTER 3.2 92 Preliminary findings
Page 102 and 103: CHAPTER 3.2 Fig. 1. Average concent
Page 104 and 105: Leja20d Leja20dSS ISAS20d 60 60 50
Page 106 and 107: CHAPTER 3.2 Table 4. Pearson correl
Page 108 and 109: Leja20d ISAS20d Makler10r 100 100 1
Page 110 and 111: CHAPTER 3.2 102 In veterinary medic
Page 112 and 113: CHAPTER 3.2 References Almeida J.,
Page 114 and 115: CHAPTER 3.2 Love C.C., Brinsko S.P.
Page 117: 3.3 Validation and usefulness of th
Page 120 and 121: CHAPTER 3.3 a camera, the sperm cel
Page 122 and 123: CHAPTER 3.3 percentage total motili
Page 124 and 125: CHAPTER 3.3 116 Agreement First, it
Page 126 and 127: Repeatability Agreement lnCONC MORF
Page 128 and 129: CHAPTER 3.3 3.3.5. Discussion 120 I
Page 131: 3.4 Need for further improvement of
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CHAPTER 3.4 3.4.3. Materials and Me
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CHAPTER 3.4 128 3.4.3.5. Statistica
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CHAPTER 3.4 Fig. 2. Scatter plots a
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CHAPTER 3.4 132 Total Motility SQA-
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CHAPTER 4 Different centrifugation
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4.1.1. Abstract CHAPTER 4.1 Three e
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CHAPTER 4.1 short time was not detr
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Table 1. Software settings of the H
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4.1.3.6.Experimental design CHAPTER
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sperm CHAPTER 4.1 EXPERIMENT 2: inf
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35 B 100 A 33 beat cross frequency
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Beat cross frequency (Hz) 40 35 30
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4.1.5. Discussion CHAPTER 4.1 In th
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References CHAPTER 4.1 Aurich C. 20
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4.2 Sperm selection using single la
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CHAPTER 4.2 resulted in an improvem
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CHAPTER 4.2 Fig. 1. Schematic prese
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CHAPTER 4.2 164 4.2.3.3.Evaluation
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CHAPTER 4.2 166 4.2.3.4. Experiment
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Table 1. Average sperm quality para
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Table 2. Descriptives of the differ
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CHAPTER 4.2 subpopulation of superi
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CHAPTER 4.2 Loomis P.R. 2001. The e
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General discussion CHAPTER 5 177
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5.1 Semen (motility) Analysis 180 S
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CHAPTER 5.1 Table 1. Criteria for g
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CHAPTER 5.1 184 The filling techniq
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CHAPTER 5.1 5.1.3. SQA-Ve in standa
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Centrifugation technique 188 5.2 Ce
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CHAPTER 5.2 A. Fig. 5. Different cu
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CHAPTER 5.2 5.2.2. Selection of spe
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5.3 Concluding remarks forward: 194
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CHAPTER 5 References Aitken R.J., B
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CHAPTER 5 Jobim M.I.M., Bustamante
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SUMMARY Horse breeding has changed
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SUMMARY sperm heads, the introducti
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SAMENVATTING De voorbije decennia h
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SAMENVATTING de SQA-Ve met software
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SAMENVATTING ♦ Het gebruikte cent
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DANKWOORD geval overtuigd dat hij w
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CURRICULUM VITAE Maarten Hoogewijs
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PUBLICATIONS IN INTERNATIONAL PEER-
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PUBLICATIONS IN NATIONAL PEER-REVIE
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BIBLIOGRAPHY Filliers M., Rijsselae
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ADDENDUM I a. b. Fig. 1. (a) Schema
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ADDENDUM I 228 Slow cooling injury
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ADDENDUM I Muldrew K., Acker J.P.,
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ADDENDUM II 232 removed by means of
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In m’n hoofd is alles heel eenvou
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