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A Area AI Artificial Insemination ALH Amplitude of the Lateral Head displacement AO Acridine Orange AR Acrosome Reacted ASMA Automated Sperm Morphology Assessment AV Artificial Vagina BCF Beat Cross Frequency CASA Computer Assisted Sperm Analysis CC Coefficient of Correlation CG Cover Glass ConA Concovalin A CONC Concentration CP Centrifugation Protocol CPA Cryoprotective Agent CSU Colorado State University CTC Chlortetracycline CV Coefficient of Variation D Depth DGC Density Gradient Centrifugation DMF Dimethylformamide DMSO Dimethyl Sulfoxide FACS Fluorescence Activated Cell Sorter FITC Fluorescein Isothiocyanate conjugated HGLL Hank’s salt solution supplemented with Hepes, glucose and lactose HOST Hypo Osmotic Swelling Test LF Leucosorb® Filtration LIN Linearity LIST OF ABBREVIATIONS iii
Page 1 and 2: AUTOMATED AND STANDARDIZED ANALYSIS
Page 3: AUTOMATED AND STANDARDIZED ANALYSIS
Page 8 and 9: LIST OF ABBREVIATIONS LM Light Micr
Page 10 and 11: PREFACE Allen W.R., 2005: The devel
Page 13: Semen analysis 1.1 5
Page 16 and 17: CHAPTER 1.1 8 In the next paragraph
Page 18 and 19: CHAPTER 1.1 a. 10 b. Fig. 1. (a) ph
Page 20 and 21: CHAPTER 1.1 Fig. 2. Different count
Page 22 and 23: CHAPTER 1.1 al., 2002), boars (Maes
Page 24 and 25: Table 2. Type of counting chamber u
Page 26 and 27: CHAPTER 1.1 staining procedures sho
Page 28 and 29: CHAPTER 1.1 Fig. 4. Drawing of equi
Page 30 and 31: CHAPTER 1.1 22 Defoin et al. (2005)
Page 32 and 33: CHAPTER 1.1 Fig. 7. Equine spermato
Page 34 and 35: CHAPTER 1.1 26 Several other techni
Page 37 and 38: 1.2.1. Semen collection CHAPTER 1.2
Page 39 and 40: CHAPTER 1.2 The Nishikawa model AV
Page 41 and 42: CHAPTER 1.2 multiple mounts are req
Page 43 and 44: CHAPTER 1.2 Recently, a new techniq
Page 45 and 46: Semen extenders CHAPTER 1.2 Many ex
Page 47 and 48: CHAPTER 1.2 extreme or last for a l
Page 49 and 50: CHAPTER 1.2 in flattened aluminum p
Page 51 and 52: Table 3. Composition of different m
Page 53 and 54: CHAPTER 1.2 superiority (Clulow et
Page 55 and 56: CHAPTER 1.2 The actual freezing tec
Page 57:
Shortcomings in current practice 1.
Page 60 and 61:
CHAPTER 1.3 1.3.2. Dose and dose ma
Page 62 and 63:
CHAPTER 1 References Aires V.A., Hi
Page 64 and 65:
CHAPTER 1 Blanc G., Magistrini M.,
Page 66 and 67:
CHAPTER 1 Devireddy R.V., Swanlund
Page 68 and 69:
CHAPTER 1 Hoflack G., Rijsselaere T
Page 70 and 71:
CHAPTER 1 Macías García B., Gonz
Page 72 and 73:
CHAPTER 1 Neuman S.L., McDaniel C.D
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CHAPTER 1 Ricker J.V., Linfor J.J.,
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CHAPTER 1 Varner D.D., Blanchard T.
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Scientific Aims CHAPTER 2 71
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CHAPTER 3 Analysis of Equine Semen
Page 87 and 88:
3.1.1. Abstract CHAPTER 3.1 The rep
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3.1.4. Results CHAPTER 3.1 Analyzin
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Totoal Motility CASA2 (%) 100 80 60
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3.2 Counting chamber type influence
Page 96 and 97:
CHAPTER 3.2 88 In addition, it has
Page 98 and 99:
CHAPTER 3.2 90 3.2.3.2. Quality ana
Page 100 and 101:
CHAPTER 3.2 92 Preliminary findings
Page 102 and 103:
CHAPTER 3.2 Fig. 1. Average concent
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Leja20d Leja20dSS ISAS20d 60 60 50
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CHAPTER 3.2 Table 4. Pearson correl
Page 108 and 109:
Leja20d ISAS20d Makler10r 100 100 1
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CHAPTER 3.2 102 In veterinary medic
Page 112 and 113:
CHAPTER 3.2 References Almeida J.,
Page 114 and 115:
CHAPTER 3.2 Love C.C., Brinsko S.P.
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3.3 Validation and usefulness of th
Page 120 and 121:
CHAPTER 3.3 a camera, the sperm cel
Page 122 and 123:
CHAPTER 3.3 percentage total motili
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CHAPTER 3.3 116 Agreement First, it
Page 126 and 127:
Repeatability Agreement lnCONC MORF
Page 128 and 129:
CHAPTER 3.3 3.3.5. Discussion 120 I
Page 131:
3.4 Need for further improvement of
Page 134 and 135:
CHAPTER 3.4 3.4.3. Materials and Me
Page 136 and 137:
CHAPTER 3.4 128 3.4.3.5. Statistica
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CHAPTER 3.4 Fig. 2. Scatter plots a
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CHAPTER 3.4 132 Total Motility SQA-
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CHAPTER 4 Different centrifugation
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4.1.1. Abstract CHAPTER 4.1 Three e
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CHAPTER 4.1 short time was not detr
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Table 1. Software settings of the H
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4.1.3.6.Experimental design CHAPTER
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sperm CHAPTER 4.1 EXPERIMENT 2: inf
Page 157 and 158:
35 B 100 A 33 beat cross frequency
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Beat cross frequency (Hz) 40 35 30
Page 161 and 162:
4.1.5. Discussion CHAPTER 4.1 In th
Page 163 and 164:
References CHAPTER 4.1 Aurich C. 20
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4.2 Sperm selection using single la
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CHAPTER 4.2 resulted in an improvem
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CHAPTER 4.2 Fig. 1. Schematic prese
Page 172 and 173:
CHAPTER 4.2 164 4.2.3.3.Evaluation
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CHAPTER 4.2 166 4.2.3.4. Experiment
Page 176 and 177:
Table 1. Average sperm quality para
Page 178 and 179:
Table 2. Descriptives of the differ
Page 180 and 181:
CHAPTER 4.2 subpopulation of superi
Page 182 and 183:
CHAPTER 4.2 Loomis P.R. 2001. The e
Page 185:
General discussion CHAPTER 5 177
Page 188 and 189:
5.1 Semen (motility) Analysis 180 S
Page 190 and 191:
CHAPTER 5.1 Table 1. Criteria for g
Page 192 and 193:
CHAPTER 5.1 184 The filling techniq
Page 194 and 195:
CHAPTER 5.1 5.1.3. SQA-Ve in standa
Page 196 and 197:
Centrifugation technique 188 5.2 Ce
Page 198 and 199:
CHAPTER 5.2 A. Fig. 5. Different cu
Page 200 and 201:
CHAPTER 5.2 5.2.2. Selection of spe
Page 202 and 203:
5.3 Concluding remarks forward: 194
Page 204 and 205:
CHAPTER 5 References Aitken R.J., B
Page 206 and 207:
CHAPTER 5 Jobim M.I.M., Bustamante
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SUMMARY Horse breeding has changed
Page 211 and 212:
SUMMARY sperm heads, the introducti
Page 213 and 214:
SAMENVATTING De voorbije decennia h
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SAMENVATTING de SQA-Ve met software
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SAMENVATTING ♦ Het gebruikte cent
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DANKWOORD geval overtuigd dat hij w
Page 223:
CURRICULUM VITAE Maarten Hoogewijs
Page 227 and 228:
PUBLICATIONS IN INTERNATIONAL PEER-
Page 229 and 230:
PUBLICATIONS IN NATIONAL PEER-REVIE
Page 231:
BIBLIOGRAPHY Filliers M., Rijsselae
Page 234 and 235:
ADDENDUM I a. b. Fig. 1. (a) Schema
Page 236 and 237:
ADDENDUM I 228 Slow cooling injury
Page 238 and 239:
ADDENDUM I Muldrew K., Acker J.P.,
Page 240 and 241:
ADDENDUM II 232 removed by means of
Page 243:
In m’n hoofd is alles heel eenvou
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