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LIST OF ABBREVIATIONS TABLE OF CONTENTS PREFACE 1 CHAPTER 1 GENERAL INTRODUCTION 3 1.1 Semen analysis 5 1.2 Collection and processing of equine semen 27 1.3 Shortcomings in current practice 49 CHAPTER 2 SCIENTIFIC AIMS 71 CHAPTER 3 ANALYSIS OF EQUINE SEMEN – IS AUTOMATION (THE) KEY TO STANDARDIZATION? 75 3.1 Influence of technical settings on CASA motility parameters of frozen-thawed stallion semen 77 3.2 Counting chamber influences the CASA motility outcomes of equine semen analysis 85 3.3 Validation and usefulness of the SQA-Ve (1.00.43) for equine semen analysis 109 3.4 Need for further improvement of SQA-Ve (1.00.61) for a univocal analysis of the quality of frozen-thawed equine semen 123 CHAPTER 4 DIFFERENT CENTRIFUGATION TECHNIQUES – TO WHAT EXTENT DO THEY AFFECT QUALITY AND PRESERVATION OF EQUINE SEMEN 135 4.1 Influence of different centrifugation protocols on equine semen preservation 137 4.2 Sperm selection using single layer centrifugation prior to cryopreservation can increase post-thaw sperm quality in stallions 157 CHAPTER 5 GENERAL DISCUSSION 177 SUMMARY 201 SAMENVATTING 205 DANKWOORD 211 CURRICULUM VITAE 215 BIBLIOGRAPHY 219 ADDENDUM I FUNDAMENTAL ASPECTS OF CRYOPRESERVATION 225 ADDENDUM II DETAILED LOADING TECHNIQUE OF THE DIFFERENT TYPES OF COUNTING CHAMBERS USED TO ANALYZE EQUINE SEMEN SAMPLES WITH CASA 231 i
Page 1 and 2: AUTOMATED AND STANDARDIZED ANALYSIS
Page 3: AUTOMATED AND STANDARDIZED ANALYSIS
Page 8 and 9: LIST OF ABBREVIATIONS LM Light Micr
Page 10 and 11: PREFACE Allen W.R., 2005: The devel
Page 13: Semen analysis 1.1 5
Page 16 and 17: CHAPTER 1.1 8 In the next paragraph
Page 18 and 19: CHAPTER 1.1 a. 10 b. Fig. 1. (a) ph
Page 20 and 21: CHAPTER 1.1 Fig. 2. Different count
Page 22 and 23: CHAPTER 1.1 al., 2002), boars (Maes
Page 24 and 25: Table 2. Type of counting chamber u
Page 26 and 27: CHAPTER 1.1 staining procedures sho
Page 28 and 29: CHAPTER 1.1 Fig. 4. Drawing of equi
Page 30 and 31: CHAPTER 1.1 22 Defoin et al. (2005)
Page 32 and 33: CHAPTER 1.1 Fig. 7. Equine spermato
Page 34 and 35: CHAPTER 1.1 26 Several other techni
Page 37 and 38: 1.2.1. Semen collection CHAPTER 1.2
Page 39 and 40: CHAPTER 1.2 The Nishikawa model AV
Page 41 and 42: CHAPTER 1.2 multiple mounts are req
Page 43 and 44: CHAPTER 1.2 Recently, a new techniq
Page 45 and 46: Semen extenders CHAPTER 1.2 Many ex
Page 47 and 48: CHAPTER 1.2 extreme or last for a l
Page 49 and 50: CHAPTER 1.2 in flattened aluminum p
Page 51 and 52: Table 3. Composition of different m
Page 53 and 54: CHAPTER 1.2 superiority (Clulow et
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CHAPTER 1.2 The actual freezing tec
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Shortcomings in current practice 1.
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CHAPTER 1.3 1.3.2. Dose and dose ma
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CHAPTER 1 References Aires V.A., Hi
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CHAPTER 1 Blanc G., Magistrini M.,
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CHAPTER 1 Devireddy R.V., Swanlund
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CHAPTER 1 Hoflack G., Rijsselaere T
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CHAPTER 1 Macías García B., Gonz
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CHAPTER 1 Neuman S.L., McDaniel C.D
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CHAPTER 1 Ricker J.V., Linfor J.J.,
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CHAPTER 1 Varner D.D., Blanchard T.
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Scientific Aims CHAPTER 2 71
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CHAPTER 3 Analysis of Equine Semen
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3.1.1. Abstract CHAPTER 3.1 The rep
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3.1.4. Results CHAPTER 3.1 Analyzin
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Totoal Motility CASA2 (%) 100 80 60
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3.2 Counting chamber type influence
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CHAPTER 3.2 88 In addition, it has
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CHAPTER 3.2 90 3.2.3.2. Quality ana
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CHAPTER 3.2 92 Preliminary findings
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CHAPTER 3.2 Fig. 1. Average concent
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Leja20d Leja20dSS ISAS20d 60 60 50
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CHAPTER 3.2 Table 4. Pearson correl
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Leja20d ISAS20d Makler10r 100 100 1
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CHAPTER 3.2 102 In veterinary medic
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CHAPTER 3.2 References Almeida J.,
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CHAPTER 3.2 Love C.C., Brinsko S.P.
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3.3 Validation and usefulness of th
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CHAPTER 3.3 a camera, the sperm cel
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CHAPTER 3.3 percentage total motili
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CHAPTER 3.3 116 Agreement First, it
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Repeatability Agreement lnCONC MORF
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CHAPTER 3.3 3.3.5. Discussion 120 I
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3.4 Need for further improvement of
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CHAPTER 3.4 3.4.3. Materials and Me
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CHAPTER 3.4 128 3.4.3.5. Statistica
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CHAPTER 3.4 Fig. 2. Scatter plots a
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CHAPTER 3.4 132 Total Motility SQA-
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CHAPTER 4 Different centrifugation
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4.1.1. Abstract CHAPTER 4.1 Three e
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CHAPTER 4.1 short time was not detr
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Table 1. Software settings of the H
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4.1.3.6.Experimental design CHAPTER
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sperm CHAPTER 4.1 EXPERIMENT 2: inf
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35 B 100 A 33 beat cross frequency
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Beat cross frequency (Hz) 40 35 30
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4.1.5. Discussion CHAPTER 4.1 In th
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References CHAPTER 4.1 Aurich C. 20
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4.2 Sperm selection using single la
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CHAPTER 4.2 resulted in an improvem
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CHAPTER 4.2 Fig. 1. Schematic prese
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CHAPTER 4.2 164 4.2.3.3.Evaluation
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CHAPTER 4.2 166 4.2.3.4. Experiment
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Table 1. Average sperm quality para
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Table 2. Descriptives of the differ
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CHAPTER 4.2 subpopulation of superi
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CHAPTER 4.2 Loomis P.R. 2001. The e
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General discussion CHAPTER 5 177
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5.1 Semen (motility) Analysis 180 S
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CHAPTER 5.1 Table 1. Criteria for g
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CHAPTER 5.1 184 The filling techniq
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CHAPTER 5.1 5.1.3. SQA-Ve in standa
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Centrifugation technique 188 5.2 Ce
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CHAPTER 5.2 A. Fig. 5. Different cu
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CHAPTER 5.2 5.2.2. Selection of spe
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5.3 Concluding remarks forward: 194
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CHAPTER 5 References Aitken R.J., B
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CHAPTER 5 Jobim M.I.M., Bustamante
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SUMMARY Horse breeding has changed
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SUMMARY sperm heads, the introducti
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SAMENVATTING De voorbije decennia h
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SAMENVATTING de SQA-Ve met software
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SAMENVATTING ♦ Het gebruikte cent
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DANKWOORD geval overtuigd dat hij w
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CURRICULUM VITAE Maarten Hoogewijs
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PUBLICATIONS IN INTERNATIONAL PEER-
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PUBLICATIONS IN NATIONAL PEER-REVIE
Page 231:
BIBLIOGRAPHY Filliers M., Rijsselae
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ADDENDUM I a. b. Fig. 1. (a) Schema
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ADDENDUM I 228 Slow cooling injury
Page 238 and 239:
ADDENDUM I Muldrew K., Acker J.P.,
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ADDENDUM II 232 removed by means of
Page 243:
In m’n hoofd is alles heel eenvou
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