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ADDENDUM I Muldrew K., Acker J.P., Elliott J.A.W., McGann L.E. 2004. The water to ice transition: implications for living cells. In: Life in the frozen state Fuller B.J., Lane N., Benson E.E. (Ed.) CRC Press, pp. 67- 108. Pegg D.E. 1984. Red cell volume in glycerol/sodium chloride/water mixtures. Cryobiology 21:234-239. Quinn P.J. 1981. The fluidity of cell membranes and its regulation. Progress in Biophysics and Molecular Biology 38:1-104. Robertson R.N. 1983. The lively membranes. Cambridge University press – Cambridge – UK. Woelders H., Chaveiro A. 2005. Theoretical prediction of ‘optimal’ freezing programmes. Cryobiologie 49:258-271. 230
ADDENDUM II DETAILED LOADING TECHNIQUE OF THE DIFFERENT TYPES OF COUNTING CHAMBERS USED TO ANALYZE EQUINE SEMEN SAMPLES WITH CASA 1. Disposable Leja, 10 µm depth, 4 chambers per slide (Leja10) – Each chamber has a volume of 1 µl, the chamber was loaded with 2.5 µl of diluted semen as described by the manufacturer. Briefly, the sample was placed carefully within the boundaries of the loading area so that the chamber was filled by capillary force. Once the sample reached the air vent at the other end of the chamber, the excess semen was carefully removed with a tip of a kimwipe tissue to prevent floating of cells. The filled chamber was left to rest for 10 seconds after which analysis was started. 2. Disposable Leja, 12 µm, 2 chambers per side (Leja12) – Each chamber has a volume of 3µl and was loaded with 5µl as described above. 3. Disposable Leja, 20 µm, 4 chambers per slide (Leja20) – Each chamber has a volume of 2 µl and was loaded with 5µl as described above. The filling time (time from pushing down the piston until the sample reached the air vent at the other end of the chamber) was recorded as described in the loading instructions, in order to be able to correct for the Segre Silberberg (SS) effect (Leja20SS). 4. Disposable ISAS, 12 µm, 4 chambers per slide (ISAS12d) – The volume of the disposable ISAS chambers is not mentioned on the slide or box. The chambers were loaded in a similar way as described for the Leja slides. Briefly, the loaded tip (5µl) of the pipette was carefully placed within the boundaries of the loading area, taking care not to touch the cover slip to prevent premature filling. The piston was pushed down allowing the chamber to be filled with capillary force, taking care that the sample stayed within the borders of the loading area. Once the sample reached the opposed air vent, excess semen form the loading area was 231
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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CHAPTER 1.2 can be induced pharmaco
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CHAPTER 1.2 centrifugation time and
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CHAPTER 1.2 36 → Colloid centrifu
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CHAPTER 1.2 of a stallion stored fo
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CHAPTER 1.2 1.2.4. Cryopreservation
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CHAPTER 1.2 0.5 mL straws, and glyc
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CHAPTER 1.2 determined if the final
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CHAPTER 1.2 46 Chemically defined e
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CHAPTER 1.2 48 Cryopreservation - t
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1.3.1. Standards for equine AI dose
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CHAPTER 1.3 The use of density grad
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CHAPTER 1 Avery B., Greve T. 1995.
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CHAPTER 1 Cheng F.-P., Fazeli A., V
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CHAPTER 1 Flipse R.J., Patton S., A
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CHAPTER 1 Kuisma P., Andersson M.,
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CHAPTER 1 Medeiros A.S.L., Gomes G.
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CHAPTER 1 Pickett B.W. 1993. Collec
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CHAPTER 1 Taberner E., Morató R.,
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CHAPTER 1 Waite J.A., Love C.C., Br
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CHAPTER 2 As evidenced in the gener
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3.1 Influence of technical settings
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CHAPTER 3.1 a CEROS (Hamilton-Thorn
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CHAPTER 3.1 Progressive Motility CA
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CHAPTER 3.1 than at least they shou
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3.2.1. Abstract CHAPTER 3.2 Sperm m
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CHAPTER 3.2 depth but not the chamb
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3.2.3.3. The different chambers and
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CHAPTER 3.2 Finally, Pearson coeffi
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90 80 70 60 50 40 30 20 10 0 drop f
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ISAS20rM Makler10r WHO 120 120 120
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60 PM Rapid 50 40 30 20 10 0 CHAPTE
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Table 5. Averages (± standard erro
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CHAPTER 3.2 sperm (tail) which coul
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CHAPTER 3.2 Hoogewijs M.K., Govaere
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CHAPTER 3.2 Spizziri B.E., Fox M.H.
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3.3.1. Abstract CHAPTER 3.3 Routine
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CHAPTER 3.3 morphological abnormali
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CHAPTER 3.3 The extended semen was
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CHAPTER 3.3 Table 1: Average values
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Repeatability Agreement SQA-Ve PM (
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References CHAPTER 3.3 Arunvipas P.
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3.4.1. Abstract CHAPTER 3.4 In this
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CHAPTER 3.4 therefore in these expe
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CHAPTER 3.4 The averages obtained a
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3.4.4.2. Experiment 2 CHAPTER 3.4 F
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References CHAPTER 3.4 Arunvipas P.
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4.1 Influence of different centrifu
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CHAPTER 4.1 4.1.2. Introduction 140
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CHAPTER 4.1 142 4.1.3.3. Semen proc
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CHAPTER 4.1 144 In the experiment 3
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CHAPTER 4.1 centrifugation (T1) and
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CHAPTER 4.1 (non CP vs CP) was sign
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35 B 80 A 33 beat cross frequency (
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CHAPTER 4.1 152 4.1.4.2. EXPERIMENT
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CHAPTER 4.1 Love and coworkers (200
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CHAPTER 4.1 Love C.C., Brinsko S.P.
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4.2.1. Abstract CHAPTER 4.2 The inc
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4.2.3. Materials and methods 4.2.3.
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CHAPTER 4.2 For the SLC group, 15 m
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CHAPTER 4.2 adding 600 µL of AO st
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4.2.3. Results 4.2.3.1. Sperm quali
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CHAPTER 4.2 The aforementioned “T
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CHAPTER 4.2 Sperm yields markedly d
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References CHAPTER 4.2 Barth A.D.,
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CHAPTER 4.2 Waite J.A., Love C.C.,
Page 187 and 188: CHAPTER 5 This thesis originated fr
Page 189 and 190: CHAPTER 5.1 Fig. 1. Schematic prese
Page 191 and 192: CHAPTER 5.1 higher than the current
Page 193 and 194: CHAPTER 5.1 al., 2007) showing a cl
Page 195 and 196: CHAPTER 5.1 treatment strategy for
Page 197 and 198: CHAPTER 5.2 supernatant allowing sp
Page 199 and 200: CHAPTER 5.2 such keeping the pellet
Page 201 and 202: CHAPTER 5.2 components of the semin
Page 203 and 204: Final reflections 5.4 Actual change
Page 205 and 206: CHAPTER 5 Dillon R.H., Fauci L.J.,
Page 207: CHAPTER 5 Suárez S.S., Osman R.A.
Page 210 and 211: SUMMARY technique that enables proc
Page 212 and 213: SUMMARY from stallions previously c
Page 214 and 215: SAMENVATTING beweeglijkheid (Hoofds
Page 216 and 217: SAMENVATTING de spermacellen gesele
Page 219 and 220: DANKWOORD Het obligatoire dankwoord
Page 221: DANKWOORD dankzij jou en ze zijn er
Page 225: CURRICULUM VITAE Maarten Hoogewijs
Page 228 and 229: BIBLIOGRAPHY Govaere J., Ducatelle
Page 230 and 231: BIBLIOGRAPHY ABSTRACTS AND PROCEEDI
Page 233 and 234: FUNDAMENTAL ASPECTS OF CRYOPRESERVA
Page 235 and 236: ADDENDUM I presence of these CPAs a
Page 237: ADDENDUM I Fig. 3. Theoretical curv
Page 241: In m’n hoofd is alles heel eenvou
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