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5.3
Concluding remarks
forward:
194
Based on the results from this thesis and literature, the following conclusions can be put
♦ When performing computer assisted sperm analysis, the motility settings will influence the
motility outcomes to a great extent.
♦ The type of counting chamber used in combination with computer assisted sperm analysis has
an important effect on the concentration and motility results. Capillarity used for filling a
chamber restricts sperm motility severely.
♦ The present version of the SQA-Ve is not accurate nor precise enough to univocally report on
equine semen quality.
♦ Centrifugation protocols have a major influence on sperm yield. High centrifugation forces (up
to 2400 × g) for a short time (5 min) will substantially reduce sperm losses without apparent
effect on in vitro sperm characteristics.
♦ Sperm selection using Androcoll-E in a single layer centrifugation technique prior to
cryopreservation, results in an overall increased sperm quality. This technique is promising for
clinical use because it enables cryopreservation of a subgroup of stallions previously deemed
as unfreezable.