view - Department of Reproduction, Obstetrics and Herd Health

35
B
80
A
33
beat cross frequency (Hz)
75
70
31
65
29
60
27
55
% membrane intact sperm
25
50
0 h 24 h 48 h 72 h
0 h 24 h 48 h 72 h
50
D
70
C
45
% progressive motility
65
40
60
% total motiliy
35
55
30
50
25
45
20
40
0 h 24 h 48 h 72 h
0 h 24 h 48 h 72 h
2400 × g 5 min
CP5
1800 × g 5 min
CP4
1200 × g 5 min
CP3
600 × g 5 min
CP2
600 × g 10 min
CP1
Fig. 2. Effect of different centrifugation protocols on percentage membrane intact sperm (A), beat cross frequency (B), total motility (C) and progressive
motility (D) during chilled storage for 72 hours.