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CHAPTER 4.1 centrifugation (T1) and 72 h (T4) after cooled storage, an aliquot of each tube was examined for DNA integrity using TUNEL assay. 146 4.1.3.7. Statistical analysis EXPERIMENT 1: influence of centrifugation protocol on function of fresh and cooled sperm 1/ sperm loss To evaluate whether the treatment (CP 1, 2, 3, 4 and 5) had an influence on sperm concentration in the aspirated supernatant, a Kruskal Wallis H test was conducted (overall treatment effect) as well as a Mann-Whitney U test (to test the difference between CP 1 versus the other treatments, separately) using SPSS software package (version 17.0, SPSS Inc., Chicago, IL). 2/ effect of centrifugation (non CP vs CP) Mixed models were fitted in SAS 9.1.3. (PROC MIXED) with centrifugation (non CP vs CP), time (T1- T4) and interaction centrifugation × time included as predictor variables. Stallion (1-5) and ejaculate (5 per stallion) were included as random effects to account for clustering of ejaculate in stallion and the repeated measurements over time, within ejaculate (including an AR(1) correlation structure), respectively. The outcome variables (percentage membrane intact sperm cells, percentage acrosome intact sperm cells, BCF, TM and PM) were normally distributed based on the inspection of QQ-plots and Kolmogorov-Smirnov tests. 3/ effect of centrifugation protocol Mixed models were fitted in SAS 9.1.3. (PROC MIXED) with CP (1-5), time (T1- T4) and the interaction CP × time included as predictor variables. Stallion (1-5) and ejaculate (5 per stallion) were included as random effects to account for clustering of ejaculate in stallion and the repeated measurements over time, within ejaculate (including an AR(1) correlation structure), respectively. The outcome variables (percentage membrane intact sperm cells, percentage acrosome intact sperm cells, BCF, TM and PM) were normally distributed based on the inspection of QQ-plots and Kolmogorov-Smirnov tests.
sperm CHAPTER 4.1 EXPERIMENT 2: influence of pre-freezing centrifugation protocol on function of thawed Mixed models were fitted in SAS 9.1.3. (PROC MIXED) with CP (1-5), ejaculate (1-5) and the interaction CP × ejaculate included as predictor variables. Stallion (1-5) was included as random effects to account for clustering of ejaculate in stallion. The outcome variables (percentage membrane intact sperm cells, percentage acrosome intact sperm cells, BCF, TM and PM) were normally distributed based on the inspection of QQ-plots and Kolmogorov-Smirnov tests. sperm EXPERIMENT 3: influence of centrifugation protocol on DNA integrity of fresh and cooled Since the percentages of DNA intact sperm cells were not normally distributed, a time effect (T1 vs T4) was analyzed using Wilcoxon signed ranks test. Effect of treatment was determined using Kruskal-Wallis test at T1 and T4. Analyses were done using SPSS software package (version 17.0, SPSS Inc., Chicago, IL). 4.1.4. Results 4.1.4.1. EXPERIMENT 1: influence of the centrifugation protocol on the function of fresh and cooled sperm The general sperm characteristics over all the ejaculates from the 5 stallions immediately after collection are presented in Table 2. There was an overall effect of CP on sperm concentration in the recovered supernatant (p
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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CHAPTER 1.2 can be induced pharmaco
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CHAPTER 1.2 centrifugation time and
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CHAPTER 1.2 36 → Colloid centrifu
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CHAPTER 1.2 of a stallion stored fo
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CHAPTER 1.2 1.2.4. Cryopreservation
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CHAPTER 1.2 0.5 mL straws, and glyc
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CHAPTER 1.2 determined if the final
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CHAPTER 1.2 46 Chemically defined e
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CHAPTER 1.2 48 Cryopreservation - t
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1.3.1. Standards for equine AI dose
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CHAPTER 1.3 The use of density grad
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CHAPTER 1 Avery B., Greve T. 1995.
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CHAPTER 1 Cheng F.-P., Fazeli A., V
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CHAPTER 1 Flipse R.J., Patton S., A
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CHAPTER 1 Kuisma P., Andersson M.,
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CHAPTER 1 Medeiros A.S.L., Gomes G.
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CHAPTER 1 Pickett B.W. 1993. Collec
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CHAPTER 1 Taberner E., Morató R.,
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CHAPTER 1 Waite J.A., Love C.C., Br
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CHAPTER 2 As evidenced in the gener
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3.1 Influence of technical settings
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CHAPTER 3.1 a CEROS (Hamilton-Thorn
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CHAPTER 3.1 Progressive Motility CA
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CHAPTER 3.1 than at least they shou
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3.2.1. Abstract CHAPTER 3.2 Sperm m
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CHAPTER 3.2 depth but not the chamb
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3.2.3.3. The different chambers and
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CHAPTER 3.2 Finally, Pearson coeffi
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Page 105 and 106: ISAS20rM Makler10r WHO 120 120 120
Page 107 and 108: 60 PM Rapid 50 40 30 20 10 0 CHAPTE
Page 109 and 110: Table 5. Averages (± standard erro
Page 111 and 112: CHAPTER 3.2 sperm (tail) which coul
Page 113 and 114: CHAPTER 3.2 Hoogewijs M.K., Govaere
Page 115: CHAPTER 3.2 Spizziri B.E., Fox M.H.
Page 119 and 120: 3.3.1. Abstract CHAPTER 3.3 Routine
Page 121 and 122: CHAPTER 3.3 morphological abnormali
Page 123 and 124: CHAPTER 3.3 The extended semen was
Page 125 and 126: CHAPTER 3.3 Table 1: Average values
Page 127 and 128: Repeatability Agreement SQA-Ve PM (
Page 129: References CHAPTER 3.3 Arunvipas P.
Page 133 and 134: 3.4.1. Abstract CHAPTER 3.4 In this
Page 135 and 136: CHAPTER 3.4 therefore in these expe
Page 137 and 138: CHAPTER 3.4 The averages obtained a
Page 139 and 140: 3.4.4.2. Experiment 2 CHAPTER 3.4 F
Page 141: References CHAPTER 3.4 Arunvipas P.
Page 145: 4.1 Influence of different centrifu
Page 148 and 149: CHAPTER 4.1 4.1.2. Introduction 140
Page 150 and 151: CHAPTER 4.1 142 4.1.3.3. Semen proc
Page 152 and 153: CHAPTER 4.1 144 In the experiment 3
Page 156 and 157: CHAPTER 4.1 (non CP vs CP) was sign
Page 158 and 159: 35 B 80 A 33 beat cross frequency (
Page 160 and 161: CHAPTER 4.1 152 4.1.4.2. EXPERIMENT
Page 162 and 163: CHAPTER 4.1 Love and coworkers (200
Page 164 and 165: CHAPTER 4.1 Love C.C., Brinsko S.P.
Page 167 and 168: 4.2.1. Abstract CHAPTER 4.2 The inc
Page 169 and 170: 4.2.3. Materials and methods 4.2.3.
Page 171 and 172: CHAPTER 4.2 For the SLC group, 15 m
Page 173 and 174: CHAPTER 4.2 adding 600 µL of AO st
Page 175 and 176: 4.2.3. Results 4.2.3.1. Sperm quali
Page 177 and 178: CHAPTER 4.2 The aforementioned “T
Page 179 and 180: CHAPTER 4.2 Sperm yields markedly d
Page 181 and 182: References CHAPTER 4.2 Barth A.D.,
Page 183: CHAPTER 4.2 Waite J.A., Love C.C.,
Page 187 and 188: CHAPTER 5 This thesis originated fr
Page 189 and 190: CHAPTER 5.1 Fig. 1. Schematic prese
Page 191 and 192: CHAPTER 5.1 higher than the current
Page 193 and 194: CHAPTER 5.1 al., 2007) showing a cl
Page 195 and 196: CHAPTER 5.1 treatment strategy for
Page 197 and 198: CHAPTER 5.2 supernatant allowing sp
Page 199 and 200: CHAPTER 5.2 such keeping the pellet
Page 201 and 202: CHAPTER 5.2 components of the semin
Page 203 and 204: Final reflections 5.4 Actual change
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CHAPTER 5 Dillon R.H., Fauci L.J.,
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CHAPTER 5 Suárez S.S., Osman R.A.
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SUMMARY technique that enables proc
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SUMMARY from stallions previously c
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SAMENVATTING beweeglijkheid (Hoofds
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SAMENVATTING de spermacellen gesele
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DANKWOORD Het obligatoire dankwoord
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DANKWOORD dankzij jou en ze zijn er
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CURRICULUM VITAE Maarten Hoogewijs
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BIBLIOGRAPHY Govaere J., Ducatelle
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BIBLIOGRAPHY ABSTRACTS AND PROCEEDI
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FUNDAMENTAL ASPECTS OF CRYOPRESERVA
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ADDENDUM I presence of these CPAs a
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ADDENDUM I Fig. 3. Theoretical curv
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ADDENDUM II DETAILED LOADING TECHNI
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In m’n hoofd is alles heel eenvou
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