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CHAPTER 3.4<br />

132<br />

Total Motility SQA-Ve (%)<br />

80<br />

60<br />

40<br />

20<br />

0<br />

SQA-Ve version 1.00.43 SQA-Ve version 1.0061<br />

Fig. 3. Scatter plots <strong>and</strong> corresponding Bl<strong>and</strong>-Altman plots for agreement <strong>of</strong> total motility obtained<br />

with CASA <strong>and</strong> SQA-Ve (version 1.00.43 <strong>and</strong> 1.00.61, respectively) for frozen-thawed equine<br />

semen ( line <strong>of</strong> perfect agreement, regression line fit the actual data).<br />

The algorithms used for motility analysis cannot be compared with the previous version to<br />

explain the differences. With further improvements, the SQA-Ve might be able to analyze motility in<br />

a more reliable way.<br />

0 20 40 60 80<br />

Total Motilty CASA (%)<br />

In conclusion, with the improved algorithms included in the newer s<strong>of</strong>tware, the SQA-Ve is<br />

able to analyze equine semen quality in a more acceptable way. However, the need remains for<br />

further improvements before this device can univocally report on thawed equine semen quality.<br />

Total Motility SQA-Ve (%)<br />

80<br />

60<br />

40<br />

20<br />

0<br />

0 20 40 60 80<br />

Total Motility CASA (%)

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