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CHAPTER 3.4
therefore in these experiments CASA was used as gold standard for comparison with both versions of
the SQA-Ve.
3.4.3.4. Experimental design
Experiment 1
For experiment 1, straws from 46 stallions were used. For motility assessment using CASA
and SQA-Ve, each sample (diluted and undiluted, respectively) was analyzed twice (T1 and T2) within
one minute with each device, so repeatability could be assessed (Fig. 1a).
For agreement, the corresponding values at T1 obtained with each device were compared.
Fig. 1. Overview of the experimental design and the number of analyses resulting in (in)complete
data.
Experiment 2
For experiment 2, straws from 50 stallions were used. For motility assessment using CASA
and SQA-Ve, each sample (diluted and undiluted, respectively) was analyzed once with each device,
to assess agreement (Fig. 1b).
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