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3.4 Need for further improvement of SQA-Ve (1.00.61) for a univocal analysis of the quality of frozen-thawed equine semen Hoogewijs M., De Vliegher S., Govaere J., De Schauwer C., Vanhaesebrouck E., de Kruif A., Van Soom A. in preparation 123
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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CHAPTER 1.2 can be induced pharmaco
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CHAPTER 1.2 centrifugation time and
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CHAPTER 1.2 36 → Colloid centrifu
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CHAPTER 1.2 of a stallion stored fo
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CHAPTER 1.2 1.2.4. Cryopreservation
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CHAPTER 1.2 0.5 mL straws, and glyc
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CHAPTER 1.2 determined if the final
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CHAPTER 1.2 46 Chemically defined e
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CHAPTER 1.2 48 Cryopreservation - t
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1.3.1. Standards for equine AI dose
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CHAPTER 1.3 The use of density grad
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CHAPTER 1 Avery B., Greve T. 1995.
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CHAPTER 1 Cheng F.-P., Fazeli A., V
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CHAPTER 1 Flipse R.J., Patton S., A
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CHAPTER 1 Kuisma P., Andersson M.,
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CHAPTER 1 Medeiros A.S.L., Gomes G.
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CHAPTER 1 Pickett B.W. 1993. Collec
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CHAPTER 1 Taberner E., Morató R.,
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CHAPTER 1 Waite J.A., Love C.C., Br
Page 81: CHAPTER 2 As evidenced in the gener
Page 85: 3.1 Influence of technical settings
Page 88 and 89: CHAPTER 3.1 a CEROS (Hamilton-Thorn
Page 90 and 91: CHAPTER 3.1 Progressive Motility CA
Page 92 and 93: CHAPTER 3.1 than at least they shou
Page 95 and 96: 3.2.1. Abstract CHAPTER 3.2 Sperm m
Page 97 and 98: CHAPTER 3.2 depth but not the chamb
Page 99 and 100: 3.2.3.3. The different chambers and
Page 101 and 102: CHAPTER 3.2 Finally, Pearson coeffi
Page 103 and 104: 90 80 70 60 50 40 30 20 10 0 drop f
Page 105 and 106: ISAS20rM Makler10r WHO 120 120 120
Page 107 and 108: 60 PM Rapid 50 40 30 20 10 0 CHAPTE
Page 109 and 110: Table 5. Averages (± standard erro
Page 111 and 112: CHAPTER 3.2 sperm (tail) which coul
Page 113 and 114: CHAPTER 3.2 Hoogewijs M.K., Govaere
Page 115: CHAPTER 3.2 Spizziri B.E., Fox M.H.
Page 119 and 120: 3.3.1. Abstract CHAPTER 3.3 Routine
Page 121 and 122: CHAPTER 3.3 morphological abnormali
Page 123 and 124: CHAPTER 3.3 The extended semen was
Page 125 and 126: CHAPTER 3.3 Table 1: Average values
Page 127 and 128: Repeatability Agreement SQA-Ve PM (
Page 129: References CHAPTER 3.3 Arunvipas P.
Page 134 and 135: CHAPTER 3.4 3.4.3. Materials and Me
Page 136 and 137: CHAPTER 3.4 128 3.4.3.5. Statistica
Page 138 and 139: CHAPTER 3.4 Fig. 2. Scatter plots a
Page 140 and 141: CHAPTER 3.4 132 Total Motility SQA-
Page 143: CHAPTER 4 Different centrifugation
Page 147 and 148: 4.1.1. Abstract CHAPTER 4.1 Three e
Page 149 and 150: CHAPTER 4.1 short time was not detr
Page 151 and 152: Table 1. Software settings of the H
Page 153 and 154: 4.1.3.6.Experimental design CHAPTER
Page 155 and 156: sperm CHAPTER 4.1 EXPERIMENT 2: inf
Page 157 and 158: 35 B 100 A 33 beat cross frequency
Page 159 and 160: Beat cross frequency (Hz) 40 35 30
Page 161 and 162: 4.1.5. Discussion CHAPTER 4.1 In th
Page 163 and 164: References CHAPTER 4.1 Aurich C. 20
Page 165: 4.2 Sperm selection using single la
Page 168 and 169: CHAPTER 4.2 resulted in an improvem
Page 170 and 171: CHAPTER 4.2 Fig. 1. Schematic prese
Page 172 and 173: CHAPTER 4.2 164 4.2.3.3.Evaluation
Page 174 and 175: CHAPTER 4.2 166 4.2.3.4. Experiment
Page 176 and 177: Table 1. Average sperm quality para
Page 178 and 179: Table 2. Descriptives of the differ
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CHAPTER 4.2 subpopulation of superi
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CHAPTER 4.2 Loomis P.R. 2001. The e
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General discussion CHAPTER 5 177
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5.1 Semen (motility) Analysis 180 S
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CHAPTER 5.1 Table 1. Criteria for g
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CHAPTER 5.1 184 The filling techniq
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CHAPTER 5.1 5.1.3. SQA-Ve in standa
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Centrifugation technique 188 5.2 Ce
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CHAPTER 5.2 A. Fig. 5. Different cu
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CHAPTER 5.2 5.2.2. Selection of spe
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5.3 Concluding remarks forward: 194
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CHAPTER 5 References Aitken R.J., B
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CHAPTER 5 Jobim M.I.M., Bustamante
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SUMMARY Horse breeding has changed
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SUMMARY sperm heads, the introducti
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SAMENVATTING De voorbije decennia h
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SAMENVATTING de SQA-Ve met software
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SAMENVATTING ♦ Het gebruikte cent
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DANKWOORD geval overtuigd dat hij w
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CURRICULUM VITAE Maarten Hoogewijs
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PUBLICATIONS IN INTERNATIONAL PEER-
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PUBLICATIONS IN NATIONAL PEER-REVIE
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BIBLIOGRAPHY Filliers M., Rijsselae
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ADDENDUM I a. b. Fig. 1. (a) Schema
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ADDENDUM I 228 Slow cooling injury
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ADDENDUM I Muldrew K., Acker J.P.,
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ADDENDUM II 232 removed by means of
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In m’n hoofd is alles heel eenvou
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