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CHAPTER 3.3 116 Agreement First, it was tested whether outcomes of results of parameters differed between methods of analysis. Therefore, mixed models were fitted in MlwiN 2.02 for the outcome variables lnCONC and MORF with “method of analysis” (SQA vs. LM) as fixed effect and “Stallion” as random effect, the latter to adjust for clustering of ejaculates within stallion. For extended semen, mixed models were fitted for the outcome variables TM and PM with “method of analysis” (CASA vs. LM and SQA vs. LM, respectively) as fixed effect and “Stallion” as random effect, the latter to adjust for clustering of ejaculates within stallion. Second, scatter plots plotting the values of SQA-Ve and CASA against LM, respectively, were created, as well as the corresponding Bland-Altman plots. 3.3.4. Results 3.3.4.1. Raw semen Repeatability of SQA-Ve None of the parameters changed significantly over time (Table 1). The repeatability of lnCONC was excellent. The linear regression line fit to the data approached the line of perfect agreement and the corresponding Bland-Altman plot had narrow 95% limits of agreement (Fig. 2). Repeatability for MORF and the three motility parameters on the other hand was rather poor as shown in Fig. 2 for MORF and PM. All CVs were acceptable (
CHAPTER 3.3 Table 1: Average values of concentration (CONC), morphology (MORF), total (TM) and progressive motility (PM), and velocity (VEL) of equine semen at time 1 (T1) and time 2 (T2) obtained with light microscopy (LM), SQA-Ve, and CASA. Within devices (SQA-Ve and CASA only) values at T1 and T2 were compared. Values with the same superscript ( a,b,c ) are significantly different. For agreement, values (at T1 only) between SQA-Ve and LM, and CASA and LM, respectively, were compared. Values with the same superscript ( ♠,♣,♥,♦ ) are significantly different. Raw semen Extended semen LM SQA-Ve LM SQA-Ve CASA T1 T1 T2 T1 T1 T2 T1 T2 CONC (×10 6 /mL) 317 289.9 288.1 - 31.8 31 39.8 39.1 lnCONC 5.66 5.55 5.55 - 3.33 3.29 3.60 a MORF (%) 67.8 ♠ 74.6 ♠ TM (%) - 74.6 73.1 80.2 ♣,♥ 3.58 a 73.7 - - - - - 73.1 ♣ 72.8 75.8 b,♥ PM (%) - 66.5 63.4 47.9 ♦ 48.7 47.4 39.8 ♦ 39.4 VEL (µm/s) - 110.4 108.2 - 80.6 78.9 99.6 c c, ♠,♣,♥,♦ p
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AUTOMATED AND STANDARDIZED ANALYSIS
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AUTOMATED AND STANDARDIZED ANALYSIS
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A Area AI Artificial Insemination A
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PREFACE The first recorded case of
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GENERAL INTRODUCTION CHAPTER 1 3
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1.1.1. Introduction CHAPTER 1.1 The
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1.1.4. Concentration CHAPTER 1.1 Ac
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Fluorescent Activated Cell Sorter C
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CHAPTER 1.1 preparation (D, µm) is
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CHAPTER 1.1 These chambers are freq
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CHAPTER 1.1 it is not possible to o
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CHAPTER 1.1 overview of common abno
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CHAPTER 1.1 head, (B5) pyriform hea
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CHAPTER 1.1 The acrosome can be eva
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CHAPTER 1.1 The sperm chromatin str
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Collection and processing of equine
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CHAPTER 1.2 30 (a) Colorado State U
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CHAPTER 1.2 can be induced pharmaco
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CHAPTER 1.2 centrifugation time and
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CHAPTER 1.2 36 → Colloid centrifu
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CHAPTER 1.2 of a stallion stored fo
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CHAPTER 1.2 1.2.4. Cryopreservation
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CHAPTER 1.2 0.5 mL straws, and glyc
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CHAPTER 1.2 determined if the final
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CHAPTER 1.2 46 Chemically defined e
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CHAPTER 1.2 48 Cryopreservation - t
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1.3.1. Standards for equine AI dose
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CHAPTER 1.3 The use of density grad
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CHAPTER 1 Avery B., Greve T. 1995.
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CHAPTER 1 Cheng F.-P., Fazeli A., V
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CHAPTER 1 Flipse R.J., Patton S., A
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CHAPTER 1 Kuisma P., Andersson M.,
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CHAPTER 1 Medeiros A.S.L., Gomes G.
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Page 75 and 76: CHAPTER 1 Taberner E., Morató R.,
Page 77: CHAPTER 1 Waite J.A., Love C.C., Br
Page 81: CHAPTER 2 As evidenced in the gener
Page 85: 3.1 Influence of technical settings
Page 88 and 89: CHAPTER 3.1 a CEROS (Hamilton-Thorn
Page 90 and 91: CHAPTER 3.1 Progressive Motility CA
Page 92 and 93: CHAPTER 3.1 than at least they shou
Page 95 and 96: 3.2.1. Abstract CHAPTER 3.2 Sperm m
Page 97 and 98: CHAPTER 3.2 depth but not the chamb
Page 99 and 100: 3.2.3.3. The different chambers and
Page 101 and 102: CHAPTER 3.2 Finally, Pearson coeffi
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Page 105 and 106: ISAS20rM Makler10r WHO 120 120 120
Page 107 and 108: 60 PM Rapid 50 40 30 20 10 0 CHAPTE
Page 109 and 110: Table 5. Averages (± standard erro
Page 111 and 112: CHAPTER 3.2 sperm (tail) which coul
Page 113 and 114: CHAPTER 3.2 Hoogewijs M.K., Govaere
Page 115: CHAPTER 3.2 Spizziri B.E., Fox M.H.
Page 119 and 120: 3.3.1. Abstract CHAPTER 3.3 Routine
Page 121 and 122: CHAPTER 3.3 morphological abnormali
Page 123: CHAPTER 3.3 The extended semen was
Page 127 and 128: Repeatability Agreement SQA-Ve PM (
Page 129: References CHAPTER 3.3 Arunvipas P.
Page 133 and 134: 3.4.1. Abstract CHAPTER 3.4 In this
Page 135 and 136: CHAPTER 3.4 therefore in these expe
Page 137 and 138: CHAPTER 3.4 The averages obtained a
Page 139 and 140: 3.4.4.2. Experiment 2 CHAPTER 3.4 F
Page 141: References CHAPTER 3.4 Arunvipas P.
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Page 148 and 149: CHAPTER 4.1 4.1.2. Introduction 140
Page 150 and 151: CHAPTER 4.1 142 4.1.3.3. Semen proc
Page 152 and 153: CHAPTER 4.1 144 In the experiment 3
Page 154 and 155: CHAPTER 4.1 centrifugation (T1) and
Page 156 and 157: CHAPTER 4.1 (non CP vs CP) was sign
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Page 160 and 161: CHAPTER 4.1 152 4.1.4.2. EXPERIMENT
Page 162 and 163: CHAPTER 4.1 Love and coworkers (200
Page 164 and 165: CHAPTER 4.1 Love C.C., Brinsko S.P.
Page 167 and 168: 4.2.1. Abstract CHAPTER 4.2 The inc
Page 169 and 170: 4.2.3. Materials and methods 4.2.3.
Page 171 and 172: CHAPTER 4.2 For the SLC group, 15 m
Page 173 and 174: CHAPTER 4.2 adding 600 µL of AO st
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4.2.3. Results 4.2.3.1. Sperm quali
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CHAPTER 4.2 The aforementioned “T
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CHAPTER 4.2 Sperm yields markedly d
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References CHAPTER 4.2 Barth A.D.,
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CHAPTER 4.2 Waite J.A., Love C.C.,
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CHAPTER 5 This thesis originated fr
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CHAPTER 5.1 Fig. 1. Schematic prese
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CHAPTER 5.1 higher than the current
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CHAPTER 5.1 al., 2007) showing a cl
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CHAPTER 5.1 treatment strategy for
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CHAPTER 5.2 supernatant allowing sp
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CHAPTER 5.2 such keeping the pellet
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CHAPTER 5.2 components of the semin
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Final reflections 5.4 Actual change
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CHAPTER 5 Dillon R.H., Fauci L.J.,
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CHAPTER 5 Suárez S.S., Osman R.A.
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SUMMARY technique that enables proc
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SUMMARY from stallions previously c
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SAMENVATTING beweeglijkheid (Hoofds
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SAMENVATTING de spermacellen gesele
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DANKWOORD Het obligatoire dankwoord
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DANKWOORD dankzij jou en ze zijn er
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CURRICULUM VITAE Maarten Hoogewijs
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BIBLIOGRAPHY Govaere J., Ducatelle
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BIBLIOGRAPHY ABSTRACTS AND PROCEEDI
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FUNDAMENTAL ASPECTS OF CRYOPRESERVA
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ADDENDUM I presence of these CPAs a
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ADDENDUM I Fig. 3. Theoretical curv
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ADDENDUM II DETAILED LOADING TECHNI
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In m’n hoofd is alles heel eenvou
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