1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...
1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...
1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...
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is shown in the following table.<br />
Extending Formulas<br />
Formula<br />
Tris Egg-Yolk<br />
Tris (g)<br />
Citric Acid, monohydrate (g)<br />
Glucose or Fructose (g)<br />
Penicillin (units/ml)<br />
Streptomycin (ug/ml)<br />
Glycerol (ml)<br />
Egg Yolk (ml)<br />
Distilled H2O to final volume (ml)<br />
Milk-Glucose<br />
Milk Powder (g) (1% fat)<br />
Glucose (g)<br />
Penicillin (units/ml)<br />
Streptomycin (ug/ml)<br />
Glycerol (ml)<br />
Distilled H2O to final volume (ml)<br />
Diluter A<br />
(w/o glycerol)<br />
21<br />
12.1<br />
6.7<br />
5.0<br />
1000.0<br />
1000.0<br />
---<br />
100.0<br />
500.0<br />
50.0<br />
0.97<br />
1000.0<br />
1000.0<br />
---<br />
500.0<br />
Diluter B<br />
(w/glycerol)<br />
12.1<br />
6.7<br />
5.0<br />
1000.0<br />
1000.0<br />
70.0<br />
100.0<br />
500.0<br />
50.0<br />
0.97<br />
1000.0<br />
1000.0<br />
70.0<br />
500.0<br />
Semen Evaluation: The volume of a fresh ejaculate was recorded immediately as measured in a<br />
calibrated collection tube. After the volume was recorded, the sample was examined for opaqueness<br />
and for any contaminants that the sample may contain.<br />
The motility of a semen sample was estimated in a properly diluted specimen so that 100<br />
individual sperm can be seen per field at 400 x under the microscope. Cool slides will reduce<br />
motility. Hot slides will kill the sperm. This procedure was used for freshly collected and extended<br />
semen.<br />
With a glass capillary tube, a small drop of semen was placed on a pre-warmed slide in a<br />
drop of sodium citrate. The two parts were mixed and covered with a cover slip, all the while on a