1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...

irreversible loss of motility within five minutes. Proper semen handling includes the following:
1. Identification of collection vials for each animal: If possible, each vial should be
permanently identified for one animal and used for only that animal. This identification
should include the number assigned to that animal, and the number of that collection. Use of
colored tape for different species of individuals assures greater accuracy.
2. Handling of sperm: Sperm handling must be done with extreme care. This includes time of
collection right up until insemination of females. When collected at either cool or warm
ambient temperatures, the semen must be insulated at all times. This includes storing
collection equipment in incubators set at approximately 37°C. Collection vials should be
covered with some type of insulating material to maintain the temperature. After collection,
these vials should be transferred to a water bath set at 37°C. If volume is to be recorded
directly after collection, a graduated collection vial makes this possible with minimum
exposure time.
Semen Extenders: Each type of extender was prepared in a 1000 ml graduated cylinder. All
crystalline materials were weighed on a triple-beam scale. All liquid materials were measured out
from a 100 ml graduated cylinder. For the TRIS extender, egg yolks were separated from the egg
whites. The yolks were then dried on a paper towel, then punctured, and allowed to drain off into the
100 ml graduated cylinder. No membranes or egg whites were allowed to contaminate the liquid
yolks. Antibiotics were crystalline powder diluted in a stock solution of 1 million units or ug/ml.
They were added to the buffer. Distilled H2O is then added to make a final volume of 500 ml of
extender. After mixing, the extenders were then poured off into 50 ml containers and frozen.
Appropriate extenders were then thawed the morning before use. The composition of all extenders
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