1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...

5. Make sure you label the two parts clearly. During the semen processing, if you add the
wrong one first, you may kill the sperm. Both parts can be stored in the refrigerator for a day,
so you can make up the extender the day before you plan to collect the buck.
V. Semen Washing Solution (Ringer Solution)
NaCl
KCl
CaCl2.2H20
Ingredients
H20 to
Amount to Make
100 ml
0.86 gm
0.03 gm
0.033 gm
1. Weigh out the ingredients listed above.
100 ml
18
1 liter
8.6 gm
0.3 gm
0.33 gm
1000 ml
2. Add 50 ml H2O (or 500 ml of H2O, if making up a liter) to the powdered ingredients.
3. Mix until all the ingredients are dissolved.
4. Add H2O to bring the total volume to 100 ml (or 1000 ml, if you are making a liter).
Semen Preparation and Extension
1. Immediately after collection, gently mix the semen with 20 parts of Ringer solution (i.e., if
the ejaculate volume is 1.2 ml add 24 ml Ringer).
2. Centrifuge the diluted semen at 1000 g for 10 minutes at room temperature.
3. After centrifugation, pour off or aspirate the supernatant (liquid) - being careful not to
disturb the pellet of sperm in the bottom of the tube.
4. Add the same amount of Ringer solution used in Step 1 and carefully re-suspend (re-