1 GOAT SEMEN COLLECTION AND PROCESSING BY DR. LOUIS ...

Semen equilibration time after addition of glycerol has been studied with conflicting results.
However, 3 to 4 hours appears sufficient for ram semen and 1½ to 3 hours equilibration time seems
adequate for goat semen. Longer equilibration times does not appear to be detrimental.
The following pages list two of the formulations which appear to work with goat semen. The
final procedure presented is the one I have used extensively with good success. I use it because of
its simplicity and results. What you use should depend upon availability of materials and
satisfactory results.
IV. Semen Extender and Procedure A
Skim milk
Glycerol
Ingredients
Penicillin g potassium
Streptomycin sulfate
17
Amount
100
7
100,000
100,000
ml
ml
units
micrograms
NOTE: The above ingredients are used to make 50 ml each of 2 extenders; PART A - nonglycerolated,
and PART B - glycerolated.
1. In a double boiler heat the 100 ml of skimmed milk to 95°C (203°F) for 10 minutes.
2. Allow the milk to cool to room temperature and divide it into two portions: 50 ml - Part A
and 43 ml Part B.
3. Add 100,000 units of penicillin G and 100,000 micrograms of streptomycin to Part A and
mix thoroughly. These antibiotics can be bought from a number of pharmaceutical firms in
pre-weighed powdered form. Your veterinarian can probably help you get the antibiotics and
figure out how to dilute them to achieve the proper concentrations.
4. Add 7 ml of glycerol to Part B (43 ml of skimmed milk) and mix.