Experimental infection and protection against ... - TI Pharma

76 Chapter 3
The subclass distribution found in vaccinated malaria naive volunteers was
similar to what was observed in exposed children [34, 35] and reflects the
expected subclass distribution for a protein antigen [36]. Antigen dose or
adjuvant only marginally influenced the subclass distribution.
In conclusion, vaccination with a single allele AMA1 vaccine induces a humoral
immune response that is biased towards the vaccine allele. The magnitude of
the response can be enhanced by a potent adjuvant, in contrast other
parameters of the humoral response like breadth, avidity and subclass
distribution appear much less influenced by the adjuvant. Future vaccine
development should focus on improving both breadth and magnitude of
antibody responses to AMA1.
Acknowledgements
The authors wish to thank Glaxo Smith Kline, Rixensart, Belgium for supplying
AS02 and SEPPIC, Paris, France for supplying Montanide ISA 720.
Funding
This work was supported by a grant from the European Malaria Vaccine
Initiative.