Experimental infection and protection against ... - TI Pharma

176 Chapter 9
Figure 1. Study design and enrolment
Immunological assessment was performed 1 day before the first immunization
(day I-1) and 1 day before the challenge infection (day C-1).A final challenge
with infectious mosquito bites was performed 28 days after the
discontinuation of chloroquine prophylaxis.
Methods
Volunteers
Fifteen healthy volunteers were recruited, aged 18-45 years old, without history
of malaria or of residence in a malaria endemic area in the six months prior to
the trial onset. Only one volunteer had ever been in an endemic area, several
years earlier. All volunteers underwent routine physical examination,
haematology and biochemistry screening at the Clinical Research Centre
Nijmegen. Serology for HIV, hepatitis B and C and asexual P. falciparum parasites
was negative in all volunteers. All volunteers gave written informed consent
prior to inclusion. The trial was approved by the Institutional Review Board of