Experimental infection and protection against ... - TI Pharma

138 Chapter 7
Figure 2. Parasite kinetics of strains NF135.C10 and NF54 assessed by
thick smear and quantitative real-time PCR. Volunteers were infected by
bites of mosquitoes infected with either NF135.C10 or NF54. (A) Percentage
of volunteers infected with NF135.C10 (red, n=5) or NF54 (black, n=5)
becoming thick smear positive, followed up up to 21 days after infection.
(B) Parasitemia of volunteers until thick smear positivity, measured by
qPCR, is shown as geometric mean and 95% confidence interval for
volunteers infected with NF135.C10 (red) and NF54 (black), and historical
controls infected with NF54 (grey area, n=48).
The drug sensitivity profile of NF135.C10 is similar to NF54 for atovaquone,
proguanil, DHA and lumefantrine, but NF135.C10 is more than 8-fold less sensitive
to chloroquine than NF54. Comparison of NF135.C10 and NF54 genotypes