Experimental infection and protection against ... - TI Pharma

NF135.C10: a new Plasmodium falciparum clone for controlled human malaria
infections
Figure 1. Genetic characterization of Plasmodium falciparum parasite isolates.
PCR was performed to assess allelic variation on (A) MSP1 K1 and MSP1 MAD20, (B)
MSP2 IC and (C) GLURP for the Pf strains NF135.C10 and NF54. Rif repetitive element
(PfRR) were characterized by the microsatellite identity test on the genomic DNA of the Pf
parasite strains (D) NF135.C10 and (E) NF54. The spectral image generated in the Gene
Mapper showing a strain-specific microsatellite fingerprint comprising a unique peak
pattern.
field strains to culture, of which 21 produced gametocytes and 16 were able to
successfully infect mosquitoes. Based on their ability to stably produce mature
gametocytes, exflagellate and to be transmitted to mosquitoes, seven strains
were cloned and two strains produced at least five oocysts and 30 000
sporozoites in more than 70% of mosquitoes after feeding. Clone NF135.C10 was
isolated by limiting dilution from isolate NF135, which was obtained from a
Dutch traveller to Cambodia diagnosed with Pf malaria in February 1993. Culture
characteristics of NF135.C10 and NF54 are displayed in Table 1.
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