Design of in vitro protein digestibility d h i l h i k assays ... - Europabio
Design of in vitro protein digestibility d h i l h i k assays ... - Europabio
Design of in vitro protein digestibility d h i l h i k assays ... - Europabio
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Modell<strong>in</strong>g Digestion –Biochemical Biochemical Models<br />
pH 2.5<br />
I=0.15<br />
Sh Shake, k 37ºC<br />
Shake Shake, 37ºC<br />
Sample over<br />
time<br />
pH 7.5<br />
I=0.15<br />
pH 6.5<br />
I=0.15<br />
Gastric mix:<br />
Stop gastric<br />
Duodenal mix:<br />
Peps<strong>in</strong><br />
digestion by Tryps<strong>in</strong>,<br />
Phosphatidyl addition <strong>of</strong> chymotryps<strong>in</strong><br />
chol<strong>in</strong>e h li vesicles il NOH NaOH Li Lipase/colipase / li<br />
Amylase<br />
Bile salts<br />
Sample over<br />
time<br />
pH 6.5<br />
I=0.15<br />
Stop duodenal<br />
digestion by<br />
addition <strong>of</strong>SBTI or<br />
PMSF