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Design of in vitro protein digestibility d h i l h i k assays ... - Europabio

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Modell<strong>in</strong>g Digestion –Biochemical Biochemical Models<br />

pH 2.5<br />

I=0.15<br />

Sh Shake, k 37ºC<br />

Shake Shake, 37ºC<br />

Sample over<br />

time<br />

pH 7.5<br />

I=0.15<br />

pH 6.5<br />

I=0.15<br />

Gastric mix:<br />

Stop gastric<br />

Duodenal mix:<br />

Peps<strong>in</strong><br />

digestion by Tryps<strong>in</strong>,<br />

Phosphatidyl addition <strong>of</strong> chymotryps<strong>in</strong><br />

chol<strong>in</strong>e h li vesicles il NOH NaOH Li Lipase/colipase / li<br />

Amylase<br />

Bile salts<br />

Sample over<br />

time<br />

pH 6.5<br />

I=0.15<br />

Stop duodenal<br />

digestion by<br />

addition <strong>of</strong>SBTI or<br />

PMSF

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