Zbornik - Društvo genetičara Srbije

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96 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Usm-7 Subotica, 30. novembar - 4. decembar 2004. UTICAJ KOMPOZICIJE GLUTENINA I PUROINDOLINA NA KVALITET SORTI HLEBNE PŠENICE D. Obreht 1 , B. Kobiljski 2 , S. Denèiæ 2 ,M.Ðan 1 i Ljiljana Vapa 1 1 Prirodno-matematièki fakultet, Novi Sad 2 Institut za ratarstvo i povrtarstvo, Novi Sad Jedinstvene karakteristike testa hlebne pšenice (T. aestivum L.) ogledaju se prvenstveno kroz elastiènost i viskoznost glutena. Kvantitativni i kvalitativni sastav glutena, koji je u visokoj meri definisan kompozicijom Glu-1 lokusa, osnovna je karakteristika pri odreðivanju tehnološkog kvaliteta hlebne pšenice. Utvrðeno je da sorte koje nose subjedinicu Bx7 OE u Glu-B1 lokusu poseduju poboljšane performanse testa. 1BL/1RS traslokacija izmeðu pšenice i rai široko se koristi u programima oplemenjivanja pšenice. Prisustvo 1RS hromatina moe imati negativan efekat na osobine testa i kvalitet pšenice. Kako tekstura endosperma zrna pšenice predstavlja vanu karakteristiku tehnološkog kvaliteta utvrðivanje alelne kompozicije puroindolinskih lokusa takoðe se primenjuje u oplemenjivaèkim programima. U ovom radu analizirana je varijabilnost HMW glutenina, prisustvo 1BL/1RS translokacije i kompozicija puroindolina u kolekciji sorti hlebne pšenice, kao i veza ovih karakteristika endosperma pšenice sa tehnološkim kvalitetom. Osnovni ciljevi istraivanja su ispitivanje uticaja mutacija puroindolinskih gena na osobine hlebnog testa i definisanje efekata koje HMW GS i 1BL/1RS translokacije imaju na tehnološki kvalitet pšenice. INFLUENCE OF GLUTENINS AND PUROINDOLINES COMPOSITION ON THE QUALITY OF BREAD WHEAT CULTIVARS Dough of bread wheat (T. aestivum L.) has unique properties, the most important of which are elasticity and viscosity of gluten. High gluten strength is used as a predictor of good quality bread wheat and it has been attributed largely to the composition of alleles at the Glu-1 loci. It has been observed that cultivars with over expressed subunit Bx7 OE at the Glu-B1 have enhanced dough strength. The 1BL/1RS wheat-rye translocation has been used extensively in wheat breeding programs. Rye 1RS chromatin can negatively impact wheat dough and bread quality. Since grain texture is also important for milling and bread making properties analysis of puroindoline loci allelic variability is also included in molecular evaluation of breeding germplasm. In this study we analyse the HMW glutenin variability, the presence of 1BL/1RS translocation and puroindoline composition in a collection of bread wheat cultivars, and its relationship with technological quality parameters. Our main objectives are to clarify if there is any relationship between puroindoline mutations and dough properties, and to compare the effects of HMW GS and 1Bl/1RS translocation in wheat technological quality.
IV-Usm-8 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 97 Subotica, 30. novembar - 4. decembar 2004. PRAVCI I METODE OPLEMENJIVANJA STOÈNOG GRAŠKA (Pisum sativum L.) U NAUÈNOM INSTITUTU ZA RATARSTVO I POVRTARSTVO U NOVOM SADU V. Mihailoviæ, A. Mikiæ, S. Katiæ, I. Pataki i Ð. Karagiæ Nauèni institut za ratarstvo i povrtarstvo, Zavod za krmno bilje, Novi Sad Primenom masovne, a potom i individualne selekcije iz prirodnih populacija, nastale su prve jugoslovenske sorte ozimog krmnog graška, NS pionir i NS dunav, od kojih je prva i dalje najraširenija u domaæoj proizvodnji. Prateæi trendove u oplemenjivanju stoènog graška u SAD, Kanadi, Zapadnoj Evropi i Sovjetskom Savezu, tokom poslednje dve decenije prošlog veka zapoèet je rad na nastanku domaæih sorti jarog stoènog graška, namenjenog kombinovanom iskorišæavanju, kao i za zrno bogato proteinima. Ukrštanjem postojeæih domaæih i inostranih genotipova sa poeljnim agronomskim svojstima i odgovarajuæim metodama selekcije, prvenstveno pedigre metodom, metodom F2 potomstva i metodom povratnog ukrštanja, stvorene su najpre sorte stoènog graška za kombinovano iskorišæavanje, NS-lim i NS-junior, a potom, istim metodama selekcije uz unošenje gena za skraæenje stabla i afila tip lista, i sorte namenjene iskljuèivo za visoke i kvalitetne prinose zrna, kao što su moravac, jezero i javor. Mikroogled sa genotipovima stoènog graška, stvorenim u Nauènom institutu za ratarstvo i povrtarstvo u Novom Sadu, bio je postavljen na oglednom polju Instituta na Rimskim Šanèevima i trajao je od 2002. do 2004. godine. Prvi deo ogleda je bio namenjen ispitivanju genetièke varijabilnosti komponenti prinosa zelene krme i ukljuèivao je ozime sorte NS pionir i NS dunav, jare sorte NS-lim, NS-junior i moravac i jare linije P-823, P-824 i P-342. Drugi deo ogleda je bio namenjen odreðivanju genetièke varijabilnosti komponenti prinosa zrna i ukljuèivao je ozime linije L-013, L-015 i L-016, jare sorte NS-lim, NS-junior, moravac, jezero i javor i jare linije P-823, P-824 i P-342. DIRECTIONS AND METHODS OF FODDER PEA (Pisum sativum L.) BREEDING AT THE INSTITUTE OF FIELD AND VEGETABLE CROPS IN NOVI SAD By means of massive and individual selection from local landraces, the first Yugoslav winter forage pea cultivars were created, NS Pionir and NS Dunav, the first still being the most widely distributed in the domestic production. Following the trends in fodder pea breeding in the USA, Canada and the Soviet Union, during two last decades of the last century a work on the creation of domestic spring fodder pea cultivars for combined use, as well as for protein-rich grain has been commenced. Crossings between domestic and introduced genotypes with desirable agronomic characteristics and appropriate methods of selection, mainly pedigree, F2 and backcross method, led to the development of the combined-use spring pea cultivars, NS-Lim and NS-Junior, and, by the same methods and intoduction of genes for shortened stems and afila leaf type, to the cultivars solely for high and quality grain yields, such as Moravac, Jezero and Javor. A small-plot trial with Novi Sad fodder pea cultivars was carried out from 2002 to 2004 at the Rimski Šanèevi Experiment Field. One part of the trial was established to study the genetic variability of forage yield components and therefore included winter cultivars NS Pionir and NS Dunav, spring cultivars NS-Lim, NS-Junior and Moravac and spring lines P-823, P-824 and P-342. The other part of the trial was aimed at the study of genetic variability of grain yield components and therefore included winter lines L-013, L-015 and L-016, spring cultivars NS-Lim, NS-Junior, Moravac, Jezero and Javor and spring lines P-823, P-824 and P-342.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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