Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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86 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Uvo-3<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
NALAENJE KANDIDATNOG GENA ZA OTPORNOST JEÈMA NA<br />
BYMOVIRUSE (BaMMV, BaYMV i BaYMV-2) PRIMENOM HROMOZOMSKOG<br />
ŠETANJA I KOMPARATIVNOG MAPIRANJA Rym4/5 LOKUSA<br />
D. Peroviæ 1,3 , N. Stein 1,3 , S. Streng 1 , B. Pellio 2 , Jelena Peroviæ 1 ,<br />
S. Stracke 1 , F. Ordon 2,3 i A. Graner 1<br />
1<br />
Institute of Plant Genetics and Crop Plant Research (IPK), Germany<br />
2<br />
Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Germany<br />
3<br />
Institute of Epidemiology and Resistance, Federal Center<br />
for Breeding Research on Cultivated Plants, Germany<br />
Jedini ekonomièan naèin zaštite jeèma od kompleksa utog virusa, u zemljama centralne<br />
Evrope, koji izazivaju pojedinaèno ili zajedno Barley mild mosaic virus (BaMMV), Barley<br />
yellow mosaic virus (BaYMV) ili BaYMV-2, je stvaranje i gajenje otpornih sorata. Recesivni<br />
lokus na dugom kraku treæeg hromozoma jeèma, predstavljen sa dva gena rym4 i rym5,<br />
korišæen je u oplemenjivaèkim programima kao izvor otpornosti. U cilju pronalaenja<br />
funkcionalne osnove recesivne otpornosti na viruse zapoèeto je hromozomsko šetanje po<br />
Rym4/5 lokusu. Izolovano je 650 kb kontinuirane BAC sekvence iz neotporne sorte Morex.<br />
Tri èetvrtine fizièke duine sekvence kosegregira sa lokusom za otpornost. Identifikovan je<br />
transkribovani homolog gena ORF1 kod eukariota. Homolozi ovog gena kod paprike i salate<br />
su nosioci otpornosti na potyviruse, pa prema tome ovaj gen je perfektan kandidat za<br />
recesivnu otpornost prema BaMMV/BaYMV. Ortologni gen je prisutan i na dugom kraku<br />
prvog hromozoma pirinèa. Dijagnostièki sekvencioni taèkasti polimorfizmi su naðeni u<br />
diferencijalnoj kolekciji jeèmova i svi od njih izazivaju aminokiselinsku zamjenu kod<br />
odgovarajuæeg proteina. Iz dobijenih rezultata moe se reæi da je u ovom radu identifikovan<br />
kandidatni gen za recesivnu otpornost jeèma prema BaMMV/BaYMV, a sve ovo nas<br />
upuæuje na moguæu funkcionalnu osnovu rym4/5 otpornosti.<br />
CHROMOSOME WALKING AND COMPARATIVE MAPPING OF THE<br />
BARLEY Rym4/5 LOCUS REVEALS THE ORF1 AS A CANDIDATE GENE<br />
FOR BYMOVIRUS RESISTANCE<br />
Breeding for resistance is the only strategy for protecting barley against barley yellow mosaic<br />
disease, which is caused in central Europe by single or combined infections with Barley mild<br />
mosaic virus (BaMMV), Barley yellow mosaic virus (BaYMV) or BaYMV-2. One recessive<br />
locus present on the long arm of chromosome 3H exhibiting the two genes rym4 and rym5 has<br />
been used as the source of resistance in European breeding programs. In order to elucidate the<br />
functional basis of recessive virus resistance, chromosome walking towards the Rym4/5 locus<br />
was initiated. A 650 kb BAC contig of the susceptible cultivar Morex was established co-segregating<br />
with three quarters of its physical extension with the resistance locus. A transcribed gene<br />
homologous to the ORF1 was identified. It provides a perfect candidate gene for recessive resistance<br />
to BaMMV/BaYMV, since homologues of this gene are involved in recessive<br />
potyvirus resistance in pepper and lettuce. An orthologous gene is present on rice chromosome<br />
1L. Diagnostic single nucleotide polymorphisms in the candidate gene were identified among a<br />
differential set of barley genotypes all leading to amino acid changes in the deduced protein,<br />
which implies a possible function in rym4/5 mediated virus resistance in barley.