Zbornik - Društvo genetičara Srbije

III-Pos-6 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 77
Subotica, 30. novembar - 4. decembar 2004.
REGENERACIJA BILJAKA IZ PROTOPLASTA DOBIJENIH IZ
HIPOKOTILA SUNCOKRETA (Helianthus annuus L.)
Ksenija Taški 1 i Dragana Vasiæ 2
1
Nacionalna laboratorija za ispitivanje semena, Novi Sad
2
Nauèni institut za ratarstvo i povrtarstvo, Novi Sad
Protoplasti dobijeni iz etioliranih hipokotila gajenog suncokreta su postavljeni u kapljice
agaroze i gajeni prema protokolu Trabace et al. (1995). Dobijeni mikrokalusi su osloboðeni
iz kapljica agaroze i prebaèeni na èvrste regeneracione podloge.
Regeneracija biljaka je dobijena na MSE regeneracionoj podlozi (Wingender et al.,
1996) nakon kratkotrajnog tretmana sa 2,2 µM Thidiazuron (TDZ). Indukcija korena je
postignuta potapanjem izdanaka u IBA rastvor. Uspešno ukorenjene biljke su cvetale.
PLANT REGENERATION FROM HYPOCOTYL PROTOPLASTS OF
SUNFLOWER (Helianthus annuus L.)
Hypocotyl protoplasts of sunflower were cultured in agarose droplets according to the
protocol of Trabace et al. (1995). Resulting micro calluses were released from agarose
and transferred onto different solid regeneration media.
Shoot regeneration was achieved by culture of calluses onto MSE regeneration medium
(Wingender et al., 1996) after treatment with 2,2 µM Thidiazuron (TDZ) for a limited period.
Freshly excised shoots were induced to root by IBA treatment. Regenerated plants
were flowered.