Zbornik - Društvo genetičara Srbije

III-Pos-4 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 75
Subotica, 30. novembar - 4. decembar 2004.
ALELNA VARIJABILNOST U LOKUSU RHT8 GENA U
NOVOSADSKOJ KOLEKCIJI PŠENICE I NJEN ZNAÈAJ U
OPLEMENJIVAÈKIM PROGRAMIMA
J. Pilipoviæ, B. Kobiljski, D. Obreht i Vladislava Galoviæ
Nauèni institut za ratarstvo i povtarstvo, Zavod za strana ita, Novi Sad
Visina stabljike pšenice je sloena osobina uslovljena velikim brojem gena kao i
uticajima spoljašnje sredine. Korišæenje gena koji redukuju visinu stabljike, a poveæavaju
prinos je jedna od glavnih strategija u dobijanju visoko prinosnih sorti hlebne pšenice.
Do sada je identofikovano 21 major gena koji redukuju visinu pšenice, a od kojih se Rht8
gen najèešæe koristi u oplemenjivaèkim programima u junoj Evropi.
Sa ciljem da se utvrdi odgovor 350 genotipova pšenice iz novosadske kolekcije na
egzogeno dodatu giberelinsku kiselinu, primenjen je GA test. Priblizno 22% od 350
genotipova nije reagovalo izduivanjem ponika na egzogeno dodatu giberelinsku
kiselinu, a 78% jeste. Alelna varijabilnost na Rht8 lokusu ovih genotipova utvðena je
analizom mikrosatelitnog lokusa MR 21, koji je blisko vezan sa Rht8 lokusom. Nakon
toga, ispitivan je efekat razlièitih alela na agronomske karakteristike i utvrðena je
znaèajna veza izmeðu alelne varijabilnosti na Rht8 lokusu sa jedne strane i visine
stabljike i komponenti prinosa sa druge strane.
U radu su dati rezultati GA testa i molekularne analize, sa specijalnim naglaskom na
potencijalnoj upotrebi ovih podataka u oplemenjivaèke svrhe.
ALLELIC VARIATION AT THE DWARFING GENE RHT8 LOCUS
IN NOVI SAD WHEAT COLLECTION AND ITS SIGNIFICANCE IN
BREEDING PROGRAMMES
The control of plant height in wheat is known to be complex, being determined by many
genes, and subject to environmental effects. The use of dwarfing genes to reduce plant
height and improve yield potential has been one of the major strategies in breeding modern,
high yielding bread wheat cultivars. So far, 21 major genes for reducing plant height
have been determined, among which Rht8 is most frequently used in wheat breeding programs
in Southern Europe.
In order to determine the respond of 350 genotypes from Novi Sad Wheat Core Collection
to exogenously applied Gibberellic Acid, GA test was performed. Approximately,
22% of 350 genotypes have shown to be GA-insensitive and 78% to be GA-sensitive. A
wheat microsatellite locus MR 21, which is closely linked to Rht8 locus, was used to
screen these wheat genotypes to assess the variation at Rht8 locus. Afterwards, effects of
different alleles on agronomic traits were analyzed and significant relation was detected
between allelic variation at Rht8 locus at one side and plant height and grain yield and its
components on the other side.
For both GA test and molecular screening, obtained results are discussed in this paper,
with special emphasis on potential use of the data for breeding purposes.