Zbornik - Društvo genetičara Srbije

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74 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE III-Pos-3 Subotica, 30. novembar - 4. decembar 2004. ANALIZA MORFOLOŠKIH KARAKTERISTIKA BILJAKA UTOG ZVEZDANA cv. BOKOR TRANSFORMISANIH SA Agrobacterium rhizogenes Radomirka Nikoliæ i Nevena Mitiæ Institut «Srbija», Centar za poljoprivredna i tehnološka istraivanja, Zajeèar Uspostavljen je efikasan metod za transformaciju utog zvezdana cv. Bokor pomoæu A. rhizogenes. Transformisani pupoljci su regenerisani na segmentima adventivnih korenova u visokoj frekvenci. Posle oiljavanja i aklimatizacije transformisane T0 biljke su gajene na eksperimentalnom polju. Izvršena je analiza morfoloških karakteristika deset sluèajno odabranih T0 biljaka utog zvezdana (genotipovi br. 2 i br. 5). One su uporeðivane sa istim osobinama kontrolnih, netransformisanih biljaka. Osobine kao što su broj stabala po biljci, duina internodija najdueg stable i broj cvetova po biljci su bile signifikantno razlièite od istih osobina kontrolnih biljaka, dok nisu utvrðene znaèajne razlike u površini listova. Nije bilo znakova prisustva genotipa sa rol genima. Transformisane biljke su imale poveæan sadraj celuloze, dok je sadraj proteina i azota bio na novou kontrolnih biljaka. ANALYSIS OF MORPHOLOGICAL TRAITS OF BIRD’S FOOT TREFOIL PLANTS cv. BOKOR TRANSFORMED WITH Agrobacterium rhizogenes An efficient method for genetic transformation and shoot regeneration was achieved in bird’s foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed T0 plants were grown in experimental field. Analysis of morphological traits and chemical content in ten unintentionally chosen T0 bird’s foot trefoil plants (genotypes no. 2 and no. 5) was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants.
III-Pos-4 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 75 Subotica, 30. novembar - 4. decembar 2004. ALELNA VARIJABILNOST U LOKUSU RHT8 GENA U NOVOSADSKOJ KOLEKCIJI PŠENICE I NJEN ZNAÈAJ U OPLEMENJIVAÈKIM PROGRAMIMA J. Pilipoviæ, B. Kobiljski, D. Obreht i Vladislava Galoviæ Nauèni institut za ratarstvo i povtarstvo, Zavod za strana ita, Novi Sad Visina stabljike pšenice je sloena osobina uslovljena velikim brojem gena kao i uticajima spoljašnje sredine. Korišæenje gena koji redukuju visinu stabljike, a poveæavaju prinos je jedna od glavnih strategija u dobijanju visoko prinosnih sorti hlebne pšenice. Do sada je identofikovano 21 major gena koji redukuju visinu pšenice, a od kojih se Rht8 gen najèešæe koristi u oplemenjivaèkim programima u junoj Evropi. Sa ciljem da se utvrdi odgovor 350 genotipova pšenice iz novosadske kolekcije na egzogeno dodatu giberelinsku kiselinu, primenjen je GA test. Priblizno 22% od 350 genotipova nije reagovalo izduivanjem ponika na egzogeno dodatu giberelinsku kiselinu, a 78% jeste. Alelna varijabilnost na Rht8 lokusu ovih genotipova utvðena je analizom mikrosatelitnog lokusa MR 21, koji je blisko vezan sa Rht8 lokusom. Nakon toga, ispitivan je efekat razlièitih alela na agronomske karakteristike i utvrðena je znaèajna veza izmeðu alelne varijabilnosti na Rht8 lokusu sa jedne strane i visine stabljike i komponenti prinosa sa druge strane. U radu su dati rezultati GA testa i molekularne analize, sa specijalnim naglaskom na potencijalnoj upotrebi ovih podataka u oplemenjivaèke svrhe. ALLELIC VARIATION AT THE DWARFING GENE RHT8 LOCUS IN NOVI SAD WHEAT COLLECTION AND ITS SIGNIFICANCE IN BREEDING PROGRAMMES The control of plant height in wheat is known to be complex, being determined by many genes, and subject to environmental effects. The use of dwarfing genes to reduce plant height and improve yield potential has been one of the major strategies in breeding modern, high yielding bread wheat cultivars. So far, 21 major genes for reducing plant height have been determined, among which Rht8 is most frequently used in wheat breeding programs in Southern Europe. In order to determine the respond of 350 genotypes from Novi Sad Wheat Core Collection to exogenously applied Gibberellic Acid, GA test was performed. Approximately, 22% of 350 genotypes have shown to be GA-insensitive and 78% to be GA-sensitive. A wheat microsatellite locus MR 21, which is closely linked to Rht8 locus, was used to screen these wheat genotypes to assess the variation at Rht8 locus. Afterwards, effects of different alleles on agronomic traits were analyzed and significant relation was detected between allelic variation at Rht8 locus at one side and plant height and grain yield and its components on the other side. For both GA test and molecular screening, obtained results are discussed in this paper, with special emphasis on potential use of the data for breeding purposes.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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III KONGRES GENETIÈARA SRBIJE Subo
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Uvodno predavanje Opening lecture
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I tematska oblast / I topic: Geneti
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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