Zbornik - Društvo genetičara Srbije

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72 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE III-Pos-1 Subotica, 30. novembar - 4. decembar 2004. NASLEÐIVANJE in vitro ODGOVORA KOD PŠENICE Nevena Mitiæ i Radomirka Nikoliæ Institut «Srbija», Centar za poljoprivredna i tehnološka istraivanja, Zajeèar Ispitivano je nasleðivanje in vitro odgovora nezrelih embriona kod pet kultivara pšenice i njihovih reciproènih hibrida. In vitro odgovor je ocenjivan na osnovu procenata formiranih kalusa, regenerativnih kalusa i broja biljaka dobijenih po embrionu. Ukrštanjem sorte ‘Vesna’ (‘VS’) sa najvišim regenerativnim potencijalom sa dvema sortama sa najniim potencijalom ‘Zajecarska 65’ (‘ZA’) i ‘Leda’ (‘LD’), pokazano je da je potencijal za regeneraciju nasledna osobina. ‘VS’ kao enski roditelj uslovio je poveæanje regenerativnog odgovora kod hibrida ‘VS’x’LD’ i ‘VS’x’ZA’, dok ‘VS’ kao muški roditelj nije ispoljio isto dejstvo. Medutim, ‘LD’ i ‘ZA’ kao muške komponente su uticale na smanjenje regenerativnog potencijala hibrida sa ‘VS’, u odnosu na samooprašenu ‘VS’. Rezulati ukazuju na postojanje klase ne-jedarnih èinilaca u kultivaru ‘VS’. Oni znaèajno doprinose relativno višoj regenerativnoj sposobnosti kalusa hibrida gde je ‘VS’ enski roditelj, u poreðenju sa onima kod kojih je ‘VS’ muški roditelj. INHERITANCE OF in vitro RESPONSE IN WHEAT The inheritance of in vitro culture response was studied by using immature embryos from five wheat cultivars and their reciprocal hybrids. In vitro culture response was evaluated according to callus formation, percentage of regenerative calli and the number of plants per embryo. By crossing the cultivar ‘Vesna’ (‘VS’) with highest tissue culture response and the two cultivars with lowest response ‘Leda’(‘LD’) and ‘Zajecarska 65’ (‘ZA’), it was demonstrated that the regeneration potential was heritable. ‘VS’ as female parent, enhanced regeneration response in hybrids ‘VS’x’LD’ and ‘VS’x’ZA’, while as a male parent, ‘VS’ did not affect the regeneration ability of hybrids ‘LD’ and ‘ZA’. However, hybrids having ‘LD’ and ‘ZA’ as a male parents exhibited a decreased regeneration potential, as compared to self-pollinated ‘VS’. The results suggest the presence of a class of extra-nuclear factors in the ‘VS’ cultivar. They significantly account for relatively higher regeneration capacity in the hybrids having this cultivar as a female parent than in those where the ‘VS’ was male parent.
III-Pos-2 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 73 Subotica, 30. novembar - 4. decembar 2004. MORFOGENETSKI POTENCIJAL ZA MIKROPROPAGACIJU HAPLOIDA ŠEÆERNE REPE Nevena Nagl, Sneana Mezei, L. Kovaèev i N. Èaèiæ Nauèni institut za ratarstvo i povrtarstvo, Novi Sad U radu je predstavljeno ispitivanje potencijalnih razlika u morfogenetskom potencijalu za mikropropagaciju osam haploidnih genotipova šeæerne repe. Haploidi su dobijeni kulturom neoploðenog semenog zametka i nakon razvoja prvog para listova postavljeni na podlogu za mikropropagaciju. Pod uticajem citokinina u podlozi kod svih genotipova došlo je do formiranja aksilarnih pupoljaka dok su se kod pojedinih genotipova razvili i adventivni pupoljci. Intenzitet multiplikacije nije bio ujednaèen i menjao se nakon svake subkultivacije, iako je kod svih genotipova dolazilo do poveæanja broja novostvorenih biljaka. Razlike u porastu broja biljaka su se poèele uoèavati nakon èetvrte subkultivacije, da bi u sladaæa dve postale još uoèljivije. Testiranjem znaèajnosti razlika izmeðu koeficijenata regresije, odnosno intenziteta porasta broja novonastalih biljaka tokom šest subkultivacija, ustanovljeno je da se oni izmeðu pojedinih genotipova znaèajno razlikuju. MORPHOGENETIC POTENTIAL FOR MICROPROPAGATION OF SUGAR BEET HAPLOIDS Here are presented results of investigation about potential differences between morphogenetic potential for micropropagation of eight sugar beet haploids. Haploids were obtained by ovule culture and after development of first leaves put on micropropagation medium. The presence of cytokinin in medium stimulated development of axillary bud, while in some genotypes adventitious bud developed as well. Multiplication rate was not consistent, although number of developed plants grew after each subcultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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III KONGRES GENETIÈARA SRBIJE Subo
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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Zbornik - Društvo genetičara Srbije

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