Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
72 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE III-Pos-1<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
NASLEÐIVANJE in vitro ODGOVORA KOD PŠENICE<br />
Nevena Mitiæ i Radomirka Nikoliæ<br />
Institut «Srbija», Centar za poljoprivredna i tehnološka istraivanja, Zajeèar<br />
Ispitivano je nasleðivanje in vitro odgovora nezrelih embriona kod pet kultivara pšenice i<br />
njihovih reciproènih hibrida. In vitro odgovor je ocenjivan na osnovu procenata<br />
formiranih kalusa, regenerativnih kalusa i broja biljaka dobijenih po embrionu.<br />
Ukrštanjem sorte ‘Vesna’ (‘VS’) sa najvišim regenerativnim potencijalom sa dvema<br />
sortama sa najniim potencijalom ‘Zajecarska 65’ (‘ZA’) i ‘Leda’ (‘LD’), pokazano je da<br />
je potencijal za regeneraciju nasledna osobina. ‘VS’ kao enski roditelj uslovio je<br />
poveæanje regenerativnog odgovora kod hibrida ‘VS’x’LD’ i ‘VS’x’ZA’, dok ‘VS’ kao<br />
muški roditelj nije ispoljio isto dejstvo. Medutim, ‘LD’ i ‘ZA’ kao muške komponente su<br />
uticale na smanjenje regenerativnog potencijala hibrida sa ‘VS’, u odnosu na<br />
samooprašenu ‘VS’. Rezulati ukazuju na postojanje klase ne-jedarnih èinilaca u kultivaru<br />
‘VS’. Oni znaèajno doprinose relativno višoj regenerativnoj sposobnosti kalusa hibrida<br />
gde je ‘VS’ enski roditelj, u poreðenju sa onima kod kojih je ‘VS’ muški roditelj.<br />
INHERITANCE OF in vitro RESPONSE IN WHEAT<br />
The inheritance of in vitro culture response was studied by using immature embryos from<br />
five wheat cultivars and their reciprocal hybrids. In vitro culture response was evaluated<br />
according to callus formation, percentage of regenerative calli and the number of plants<br />
per embryo. By crossing the cultivar ‘Vesna’ (‘VS’) with highest tissue culture response<br />
and the two cultivars with lowest response ‘Leda’(‘LD’) and ‘Zajecarska 65’ (‘ZA’), it<br />
was demonstrated that the regeneration potential was heritable. ‘VS’ as female parent,<br />
enhanced regeneration response in hybrids ‘VS’x’LD’ and ‘VS’x’ZA’, while as a male<br />
parent, ‘VS’ did not affect the regeneration ability of hybrids ‘LD’ and ‘ZA’. However,<br />
hybrids having ‘LD’ and ‘ZA’ as a male parents exhibited a decreased regeneration potential,<br />
as compared to self-pollinated ‘VS’. The results suggest the presence of a class of<br />
extra-nuclear factors in the ‘VS’ cultivar. They significantly account for relatively higher<br />
regeneration capacity in the hybrids having this cultivar as a female parent than in those<br />
where the ‘VS’ was male parent.