Zbornik - Društvo genetičara Srbije

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6 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE Subotica, 30. novembar - 4. decembar 2004. Uvodno predavanje VEŠTAÈKI HROMOZOM BAZIRAN NA SATELITSKOJ DNK Gyula Hadlaczky Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary In vivo konstrukcija animalnog veštaèkog hromozoma pretstavlja ponovljivu i efikasnu tehnologiju za konstrukciju stabilnog veštaèkog hromozoma baziranog na satelitskoj DNK (SATACs – Stable satelite DNA-based Artificial Chromosomes) definisanog genetièkog sadraja. SATAC tehnologija se bazira na indukciji de novo formiranja hromozoma amplifikacijom na velikoj skali, koja moe da se inicira ciljnom integracijom strane DNK u satelit/rDNK region hromozoma domaæina. Ko-amplifikacija sekvenci integracionog mesta ima kao rezultat de novo formiranje hromozomskih krakova i nove hromozome koji imaju sekvence satelitske rDNK i egzogene DNK. SATACssu heterohromatinski, ali obezbeðuju pogodnu hromozomalnu okolinu za stabilnu, trajnu ekspresiju integrisanog genetièkog materijala. SATACs mogu biti konstruisani, preèišæeni i preneseni u æeliju – primaoca ukljuèujuæi oploðenu jajnu æeliju. Transgene ivotinje su uspešno kreirane preèišæenim SATACs i utvrðena je vertikalna transmisija Zbog osnovne sliènosti elementarnih mehanizama u æelijama eukariota, SATAC tehnologija moe da se uspešno primeni kod razlièitih vrsta, ukljuèujuæi ljudske, animalne i biljne æelije za in vivo konstrukciju veštaèkog hromozoma sa odabranim sekvencama. SATELLITE DNA-BASED ARTIFICIAL CHROMOSOMES In vivo generation of mammalian artificial chromosomes represents a reproducible and efficient technology for construction of stable satellite DNA-based artificial chromosomes (SATACs) with defined genetic content. SATAC technology is based on the induction of de novo chromosome formations via large-scale amplification, which can be initiated by targeted integration of exogenous DNA into the satellite/rDNA region of host chromosomes. Co-amplification of sequences of the integration site results in de novo formed chromosome arms and new chromosomes that composed of exogenous DNA and satellite/rDNA sequences. SATACs are heterochromatic, however, they provide a suitable chromosomal environment for stable, persisting expression of the integrated exogenous genetic material. SATACs can be engineered, purified and transferred into recipient cells including fertilized eggs. Transgenic animals have successfully been generated with purified SATACs, and the transmission of artificial chromosome through generations has been demonstrated. Because of the basic similarities of the intrinsic cellular mechanisms between eukaryotes, SATAC technology can successfully be applied in different species including human, animal, and plant cells for in vivo construction of satellite DNA based artificial chromosomes from predictable sequences. Due to the rapid development of SATAC technology, by the development of the Artificial Chromosome Expression System, the feasibility of the use of satellite DNA-based artificial chromosome has been established, in different fields of gene technology. Second generation artificial chromosomes represent a novel protein production platform both for cellular protein production and for production of therapeutic molecules in body fluids of transgenic animals; for development of transgenic animals with genetically modified (humanized) tissues and organs for xenotransplantation. Also, stable SATACs with practically unlimited carrying capacity may serve as potential vectors for animal breeding, and for human gene therapy.
I tematska oblast / I topic: Genetièke osnove i zaštita biodiverziteta Genetic basis and protection of biodiversity Uvodna izlaganja / Introductory lectures I-Uvo
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Kovaèev L. 73, 90, 125, 140, 147 K
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Stanojeviæ B. 223 Stanojeviæ J. 2
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Zbornik - Društvo genetičara Srbije

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