Zbornik - Društvo genetičara Srbije

More documents

Recommendations

Info

52 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE II-Usm-5 Subotica, 30. novembar - 4. decembar 2004. STRUKTURNA I FUNKCIONALNA ANALIZA PROMOTORA HUMANOG SOX14 GENA Jelena Ðuroviæ, Mina Seoviæ, Isidora Petroviæ i Milena Stevanoviæ Institut za molekularnu genetiku i genetièko inenjerstvo, Beograd SOX14 je èlan SOX familije gena koji kodiraju transkripcione regulatore ukljuèene u kontrolu procesa razviæa. Poznato je da su mutacije u SOX genima odgovorne za pojavu genetièkih sindroma kod èoveka, što ukazuje na njihovu vanu ulogu u procesu razviæa. I ako ekspresioni profil SOX gena ukazuje da je njihova ekspresija specifièna za tkivo i stupanj razviæa, vrlo malo se zna o mehanizmima koji regulišu ekspresiju ovih gena. U cilju prouèavanja molekularnih mehanizama koji regulišu transkripciju humanog SOX14 gena, odreðen je start transkripcije metodom elongacije prajmera. Dobijena su dva produkta elongacije prajmera duine 275 nukleotida i 194 nukleotida. Ovi rezultati ukazuju da transkripcija otpoèinje od guanina na poziciji -251 bp u odnosu na start kodon, mada postojanje starta transkripcije na poziciji -170 ne moe biti iskljuèeno. Identifikacija regiona koji pokazuje bazalnu promotorsku aktivnost kao i proksimalni enhenser, odreðeni su sposobnošæu konstrukata sa 5’ nekodirajuæim regionom SOX14 gena da indukuju ekspresiju CAT reporter gena. Ovim eksperimentima pokazano je da je region izmeðu -470 i +201 odgovoran za bazalnu aktivnost SOX14 promotora. NF-Y je transkripcioni faktor vaan za regulaciju transkripcije gena koji ne poseduju TATA boks. U ovom radu pokazano je da se NF-Y vezuje za CCAAT motiv prisutan u promotoru SOX14 gena. Mutacionom analizom pokazano je da je CCAAT motiv funkcionalan i znaèajan za regulaciju transkripcije SOX14 gena. STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THE HUMAN SOX14 PROMOTER SOX14 is a member of SOX gene family of putative transcriptional regulators implicated in the control of diverse developmental processes. Mutations in SOX genes are known to be responsible for human genetic syndromes, demonstrating the critical role that they play in normal embryonic development. Although SOX gene expression patterns suggest tissue- and development stage-specific control of gene expression, little is known about mechanisms responsible for expression of these genes. In order to elucidate the molecular mechanisms controlling the expression of the human SOX14 gene, we have determined the transcription start site using primer extension method and carried out the structural and functional analysis of the regulatory region responsible for its expression. Two distinct primer extension products were identified: the single major product that migrates with a length of 275 nt and the minor product 194 nt in size. This result indicates that the transcription of the SOX14 initiates at the single major site at the guanine residue 251 bp upstream of the start codon, although the existence of a minor transcription start site can not be excluded. To identify the DNA regions responsible for the control of SOX14 gene transcription we have analyzed the ability of truncated fragments from the 5’ flanking region of the SOX14 gene to drive expression of CAT reporter gene. Functional mapping of the 5’ regulatory region revealed that the sequence between -470 and +201 bp is essential for minimal basal activity of the SOX14 promoter. NF-Y is a transcriptional factor involved in transcription of TATA-less genes. We have identified that NF-Y binds to the CCAAT box motif present in the regulatory region of the SOX14 promoter. By mutation analysis we have shown that CCAAT box motif present in the SOX14 promoter plays a functional role in the transcription of this gene.
II-Usm-6 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 53 Subotica, 30. novembar - 4. decembar 2004. CITOGENETIÈKI EFETKI TIAMULINA S IN VIVO Biljana Markoviæ 1 , N. Ðeliæ 2 , Z. Stanimiroviæ 1 , M. Anðelkoviæ 2 i D. Marinkoviæ 3 1 Katedra za Biologiju, Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd 2 Institut za zoologiju, Biološki fakultet, Univerzitet u Beogradu, Beograd 3 Srpska akademija nauka i umetnosti, Beograd U ovom radu ispitivan je citogenetièki efekat antibiotskog preparata Tiamulina S (proizvoðaè Hemovet, Vršac), koji ima široku primenu u veterinarskoj medicini. Eksperiment in vivo obavljen je kod laboratorijskog miša BALB/c soja praæenjem uticaja intragastriène aplikacije Tiamulina S na nastanak hromozomskih promena u æelijama kostne sri u mitozi i semenim æelijama u mejozi. Klastogeni efekat Tiamulina S praæen je u tri eksperimentalne doze (0,01 ml/kg, 0,2 ml/kg i 0,4ml/kg). Eksperimenti su obavljeni po smernicama OECD o testiranju na genotoksiènost. Statistièka analiza uraðena je Studentovim t-testom. Eksperimentalni rezultati pokazuju da ispitivani antibiotiski preparat Timulin S ima sposobnost promene kariotipa æelija kostne sri i testisa i indukcije numerièkih hromozomskih aberacija tipa aneuploidija i poliploidija, kao i strukturnih hromozomskih promena tipa lezija, prekida i Robertsonovih translokacija. Pri praæenju zastupljenosti ukupnih citogenetièkih promena u æelijama kostne sri i testisa mogu se izvesti dva zapaanja: prisustvo visoko signifikantnih razlika (p
Page 1 and 2: DRUŠTVO GENETIČARA SRBIJE SERBIAN
Page 3 and 4: III KONGRES GENETIÈARA SRBIJE Subo
Page 5 and 6: Uvodno predavanje Opening lecture
Page 7 and 8: I tematska oblast / I topic: Geneti
Page 9: I-Uvo-2 ZBORNIK ABSTRAKATA III KONG
Page 12 and 13: 12 ZBORNIK ABSTRAKATA III KONGRESA
Page 43 and 44: II tematska oblast / II topic: Stru
Page 45 and 46: II-Uvo-2 ZBORNIK ABSTRAKATA III KON
Page 49 and 50: II-Usm-2 ZBORNIK ABSTRAKATA III KON
Page 51: II-Usm-4 ZBORNIK ABSTRAKATA III KON
Page 57 and 58: II-Pos-2 ZBORNIK ABSTRAKATA III KON
Page 63 and 64: III tematska oblast / III topic: Ge
Page 65: III-Uvo-2 ZBORNIK ABSTRAKATA III KO
Page 89 and 90: IV tematska oblast / IV topic: Ople
Page 91 and 92: IV-Usm-2 ZBORNIK ABSTRAKATA III KON
Page 99 and 100: IV-Usm-10 ZBORNIK ABSTRAKATA III KO
Page 101 and 102: IV-Usm-12 ZBORNIK ABSTRAKATA III KO
Page 103 and 104:
IV-Usm-14 ZBORNIK ABSTRAKATA III KO
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
IV tematska oblast / IV topic: Ople
Page 119 and 120:
IV-Pos-2 ZBORNIK ABSTRAKATA III KON
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
IV-Pos-10 ZBORNIK ABSTRAKATA III KO
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
V tematska oblast / V topic: Geneti
Page 181:
V-Uvo-2 ZBORNIK ABSTRAKATA III KONG
Page 184 and 185:
184 ZBORNIK ABSTRAKATA III KONGRESA
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 203 and 204:
V tematska oblast / V topic: Geneti
Page 205 and 206:
V-Pos-2 ZBORNIK ABSTRAKATA III KONG
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
V-Pos-10 ZBORNIK ABSTRAKATA III KON
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
ZAVRŠNO PREDAVANJE CLOSING LECTURE
Page 229 and 230:
A Aksentijeviæ I. 194 Aleksiæ J.
Page 231 and 232:
Kovaèev L. 73, 90, 125, 140, 147 K
Page 233 and 234:
Stanojeviæ B. 223 Stanojeviæ J. 2
show all

Zbornik - Društvo genetičara Srbije

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?