Zbornik - Društvo genetičara Srbije

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48 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE II-Usm-1 Subotica, 30. novembar - 4. decembar 2004. ANALIZA SASTAVA MIKROBIOLOŠKIH ZAJEDNICA RIZOSFERA Ramonda nathaliae I Ramonda serbica Ivana Bratiæ, M. Saviæ, Lidija Ðokiæ i Branka Vasiljeviæ Institut za molekularnu genetiku i genetièko inenjerstvo, Beograd Ramonda nathaliae i Ramonda serbica su tercijalni relikti i endemiti Balkanskog poluostrva koji rastu u pukotinama stena na strmim kreènjaèkim liticama u Siæevaèkoj klisuri (Srbija). Cilj ovog rada je bio da se utvrdi sastav mikrobioloških zajednica rizosfera R. nathalia i R. serbica metodama PCR (reakcija lanèane polimerizacije) i RFLP (polimorfizam duina restrikcionih fragmenata). Za PCR metodu je korišæen univerzalni par prajmera koji selektivno amplifikuje fragment bakterijskog 16S rRNA gena. PCR fragmenti (duine oko 1300bp) su dobijeni iz ukupne DNA izolovane iz rizosfera R. serbica i R. nathaliae. PCR produkti su potom klonirani u pUC19 vektor i restrikciono analizirani pomoæu RsaI enzima. Restrikcioni profili su potvrdili prisustvo 89 razlièitih operativnih taksonomskih jedinica (OTJ) poreklom iz rizosfere R. nathaliae, a samo 13 razlièitih OTJ poreklom iz rizosfere R. serbica. STUDY OF MICROBIAL COMMUNITY COMPOSITION FROM RHIZOSPHERE SOILS OF Ramonda nathaliae AND Ramonda serbica Ramonda nathaliae i Ramonda serbica, the Tertiary relict and the endemic species of the Balkan, grow in the rock fissures on steep limestone cliffs of the Sicevo gorge (Serbia). The aim of this study was to analyse microbial community composition of rhizosphere soils of R. nathaliae and R. serbica by PCR (polymerase chain reaction) and RFLP (restriction fragment length polymorfism) methods. A universal primer pair for PCR was designed to selectively amplify a fragment of bacterial 16S rRNA gene. PCR fragments (approximately 1300 bp) were amplified from total DNA that was isolated from rhizosphere soils of R. serbica and R. nathaliae. PCR products were cloned into pUC19 plasmid and clones were subjected to restriction analysis employing restriction enzyme RsaI. Restriction profiles indicated the presence of 89 different operative taxonomic units (OTU) in the rhizosphere soil of R. nathalia, but only 13 different OTUs from the rhizosphere soil of R. serbica.
II-Usm-2 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 49 Subotica, 30. novembar - 4. decembar 2004. EFEKTI B HROMOZOMA KOD UTOGRLOG ŠUMSKOG MIŠA, Apodemus flavicollis (Rodentia, Mammalia) – NIVO MORFOLOŠKE INTEGRACIJE MANDIBULE Vida Jojiæ 1 , Jelena Blagojeviæ 1 , Ana Ivanoviæ 2 i M. Vujoševiæ 1 1 Odeljenje za genetiku, Institut za biološka istraivanja «Siniša Stankoviæ», Beograd 2 Institut za zoologiju, Biološki fakultet, Beograd B hromozomi (B) predstavljaju prekobrojne hromozome široko zastupljene kod mnogih biljnih i ivotinjskih vrsta. U pogledu efekata ovih hromozoma na fenotipskom nivou postoje velike razlike meðu vrstama kao i mali broj registrovanih sluèajeva gde prisustvo Bs dovodi do kvalitativnih i/ili kvantitativnih promena fenotipa. Cilj rada bio je utvrðivanje potencijalnih efekata B na morfološku varijabilnost mandibule kod jedinki populacije A. flavicollis sa Avale. Korišæenjem fenotipskih korelacija meðu mandibularnim karakterima procenjen je nivo morfološke integracije mandibule kod dve grupe jedinki (sa i bez B), kao i kod 4 grupe jedinki (mujaci i enke sa i bez B). Mandibula glodara predstavlja kompleksan skeletni organ sastavljen od dva funkcionalno i razvojno razlièita dela (corpus, koji podupire korene zuba i ascending ramus, preko koga se mišiæi vezuju za donju vilicu). Stepen morfološke integracije izraen preko indeksa integracije (I) procenjen je na tri nivoa - corpus, ascending ramus i èitava mandibula. Sve tri vrednosti indeksa integracije bile su veæe kod grupe jedinki sa B. Najizraenije poveæanje stepena morfološke integracije registrovano je kod enki sa B. Dobijeni rezultati ukazuju ne samo da prisustvo B kod utogrlog šumskog miša proizvodi efekte na nivou fenotipa, veæ i da je doprinos B poveæanju nivoa morfološke integracije razlièit kod razlièitih polova. THE EFFECTS OF B CHROMOSOMES IN YELLOW-NECKED MOUSE, Apodemus flavicollis (Rodentia, Mammalia) - THE LEVEL OF MORPHOLOGICAL INTEGRATION OF MANDIBLE B chromosomes (Bs) are supernumerary chromosomes widely distributed among many species of plants and animals. From the aspect of their phenotypic effects, species possessing Bs are particularly diverse. In general, there is the scarcity of reported cases where the presence of Bs influences phenotypes qualitatively and/or quantitatively. Our goal was to search for the potential effects of Bs on morphological variation of mandible in A. flavicollis from Avala population. We’ve choosed to study phenotypic correlations among mandibular traits in order to estimate the level of their morphological integration for both groups of animals (with and without Bs), as well as for divided sample according to sex and presence of Bs (meles and females with and without Bs).. Bearing in mind that rodent mandible is a complex skeletal organ consisting of two functional and developmental units (corpus, supporting tooth roots and ascending ramus, participating in the attachments of the muscles) we estimated morphological integration by index of integration (I) on the three levels: overall mandible, corpus unit and ascending ramus unit. We found that individuals with Bs showed higher level of morphological integration for both units and for overall mandible as well. The highest increase in the level of morphological integration showed females with Bs. These results indicate that presence of Bs not only produces the effects in yellow-necked mouse, but also the contribution of Bs to increase level of morphological integration is different in different sexes.
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Zbornik - Društvo genetičara Srbije

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