Zbornik - Društvo genetičara Srbije

I-Pos-20 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 41
Subotica, 30. novembar - 4. decembar 2004.
VARIJABILNOST AMINOKISELINSKOG SASTAVA KOD SORTI JEÈMA
Nevena Ðukiæ 1 , D. Kneeviæ 2 , Gordana Matiæ 3 ,
Marina Stamenkoviæ-Radak 3 i M. Anðelkoviæ 3
1
Prirodno-matematièki fakultet, Institut za biologiju, Kragujevac
2
Institut za istraivanja u poljoprivredi SRBIJA, Centar za strna ita, Kragujevac
3
Biološki fakultet, Beograd
U ovom radu ispitivano je 29 sorti jeèma. Metodom hromatografije analizirano je prisustvo
slobodnih aminokiselina, a spektrofotometrijski odreðena je njihova koncentracija.
Kvalitativna analiza aminokiselinskog sastava ukazuje da su najzastupljenije meðu
slobodnim aminokiselinama zrna ispitivanih sorti jecma: glutaminska kiselina, prolin,
valin, treonin i druge. Obzirom da jeèam ima svoju upotrebnu vrednost u stoènoj ishrani,
u proizvodnji slada i piva, kao i za specijalne namene u ishrani ljudi, veoma je vano
prisustvo esencijalnih aminokiselina. U razlièitim sortama ispitivanog jeèma identifikovali
smo sedam esencijalnih aminokiselina (valin, treonin, leucin, fenilalanin,
metionin, triptofan i arginin).
Kvantitativna analiza slobodnih aminokiselina ukazuje da je njihov sadraj visok u
sledeæim sortama jeèma: Novosadski 519 i Novosadski 535 (193 mg/ml), Novosadski 703
(163 mg/ml), Novosadski 293, Novosadski 309 i Gigant (161 mg/ml). Analiza sadraja
glutaminske kiseline u zrnu jeèma pokazala je da je ona kvantitativno najzastupljenija
aminokiselina. Koncentracija glutaminske kiseline kod sorte Novosadski 319 je 86
mg/ml; a kod sorti jeèma Novosadski 529 i Novosadski 535 koncentracija je 63,5 mg/ml,
odnosno 62 mg/ml.
VARIJABILITY OF AMINOACID CONTENTS IN BARLEY CULTIVARS
Study of presence of free amino acids in 29 barley cultivars have been analyzed by chromatography
and their concentrtation were determined by spectrophotometry. Qualitative
analysis of amino acid indicated that glutamic acid, proline, valine, threonine were most
present among free amino acid in grain of examined barley cultivars. The presence of
esential amino acid is very important, considering that barley use for feed animals, production
of malt and beer, as well as for special purpose in human nutrition. In different
barley cultivars was identified 7 esential aino acids (valine, threonine, leucine,
phenylalanine, methionine, triptophan and arginine). Analysis of free amino acid indicated
that their contents was very high in next cultivars: Novosadski 519 and Novosadski
535 (193 mg/ml); than Novosadski 703 (163 mg/ml), Novosadski 293, Novosadski 309
and Gigant (161 mg/ml). The analysis of contents of glutamic acid showed that its most
present amino acid in barley grain. Concentration of glutamic acid in cultivar Novosadski
319 is 86 mg/ml; and in barley cultivars Novosadski 529 and Novosadski 535 with concentration
63,5 mg/ml, and 62 mg/ml, respectively.