Zbornik - Društvo genetičara Srbije

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28 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE I-Pos-7 Subotica, 30. novembar - 4. decembar 2004. IZOLACIJA I KARAKTERIZACIJA BAKTERIJA MLEÈNE KISELINE IZ ZLATARSKOG SIRA Katarina Krstiæ 1 , Amarela Terziæ-Vidojeviæ 1 , Maja Vukašinoviæ 2 , Jelena Lozo 1 , Ð. Fira 1 i Lj. Topisiroviæ 1 1 Institut za molekularnu genetiku i genetièko inenjerstvo, Beograd 2 Tehnološko-metalurški fakultet, Beograd, Sojevi bakterija mleène kiseline (BMK) izolovani su iz sveeg mleka i sira razlièitog perioda zrenja (1, 10 20, 30, 45 i 60 dana). Zlatarski sir je napravljen u domaæinstvu bez dodavanja starter kulture. Ukupno je izolovano 253 isolata. Korišæenjem tradicionalnih mikrobioloških metoda determinisano je 138 isolata kao Lactobacillus sp. i 114 izolata kao Lactococcus sp. Za dalju determinaciju isolata do nivoa vrste primeniæemo molekularno genetièke metode kao što su SDS-PAGE, AFLP i rep-PCR. Analizom BMK iz ove kolekcije pokazano je da neki od izolata produkuju antimikrobne supstance, ekstracelularne proteinaze i imaju sposobnost formiranja multicelularnih agregata. Izuèavanjem organizacije gena, koji kodiraju proteinaze, bakteriocine i koji agregiraju, moe se omoguæiti konstrukcija specifiènih starter kultura za dobijanje autohtonih fermentisanih mleènih proizvoda, tj. sa geografskim poreklom. Intenzivnim prouèavanjem molekularne genetike BMK iz prirodnih izolata otvaraju se sasvim novi potencijali u industriji hrane. ISOLATION AND CHARACTERISATION OF LACTIC ACID BACTERIA FROM ZLATAR’S CHEESE Strains of lactic acid bacteria (LAB) have been isolated from raw milk and cheese during different periods of ripening (1, 10, 20, 30, 45 and 60 days). Homemade cheese from Zlatar has been manufactured without addition of any starter culture. A total number of isolated LAB was 253. Using traditional microbiological methods we have determined 138 isolates as Lactobacillus sp. and 114 isolates as Lactococcus sp. For further determination of the isolates to the species level, we will apply molecular genetic methods like SDS-PAGE, AFLP and rep-PCR. Analysis of LAB from this collection showed that some of them produced antimicrobial substances, extracellular proteinases and had ability to form multicellular aggregates. Elucidation of the organization of genes encoding proteinases, bacteriocins and aggregation factors could be used in the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. products with geographical origin. The extensive studies on molecular genetics of LAB from natural sorces opens new potentials in food industry.
I-Pos-8 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 29 Subotica, 30. novembar - 4. decembar 2004. GENETIÈKA KARAKTERIZACIJA LOKALNIH POPULACIJA KUKURUZA POMOÆU SSR MARKERA K. Markoviæ, D. Ignjatoviæ-Miciæ i V. Laziæ-Janèiæ Institut za kukuruz «Zemun Polje», Beograd-Zemun Banka gena Instituta za kukuruz «Zemun Polje» poseduje preko dve hiljade lokalnih populacija kukuruza, klasifikovanih i okarakterisanih na morfološkom nivou. Da bi se omoguæila preciznija karakterizacija i izbegla pojava potencijalnih duplikata u kolekciji neophodna je analiza postojeæih genotipova primenom molekularnih markera. U ovom radu analizirana je 21 lokalna populacija kukuruza pomoæu 30 SSR markera. Korišæena je metoda grupnih uzoraka, gde je svaka populacija predstavljena sa po 30 biljaka. Elektroforetsko razdvajanje amplifikovanih fragmenata je raðeno na agaroznom i denaturišuæem poliakrilamidnom gelu. Poliakrilamidni gelovi su bojeni srebro-nitratom. Statistièka analiza dobijenih podataka je uraðena pomoæu NTSYSpc2 programskog paketa, a za izraèunavanje genetièkih distanci korišæena je «Modifikovana Roderova Distanca» na osnovu izraèunatih alelnih frekvencija unutar populacija. Rezultati i diskusija æe biti prikazani na posteru. GENETIC CHARACTERIZATION OF LOCAL MAIZE POPULATIONS WITH SSR MARKERS The genebank in Maize Research Institute «Zemun Polje» maintains the collectin of over 2000 local maize populations classified and characterised at the morphological level. Molecular marker application would enable more precise characterisation and avoid occurrence of duplicate accessions, thus supporting the maintenance of the collection. Thirty SSR markers were used to analyse twenty one local maize populations. DNA pooling strategy was done with thirty plants representing each population. Amplified fragments were separated both on denaturing polyacrilamide and agarose gels. Silver staining was performed on polyacrilamide gels. NTSYSpc2 programe package was applied for statistical analysis of the results. Genetic distance was calculated using Modified Rogers Distance based on allelic frequency scoring of the bands within accessions. Results and discussion will be presented on the poster.
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Zbornik - Društvo genetičara Srbije

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