Zbornik - Društvo genetičara Srbije

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26 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE I-Pos-5 Subotica, 30. novembar - 4. decembar 2004. IZOLACIJA I KARAKTERIZACIJA BAKTERIJA MLEÈNE KISELINE IZ RADANSKOG SIRA Nataša Jokoviæ 1 , Maja Vukašinoviæ 2 i Lj. Topisiroviæ 3 1 Tehnološki fakultet, Leskovac 2 Tehnološko-metalurški fakultet, Beograd 3 Institut za molekularnu genetiku i genetièko inenjerstvo, Beograd Iz kravljeg i ovèijeg mleka kao i iz uzoraka sira tokom zrenja (5, 10, 20, 30 i 60 dana) izolovano je 118 izolata bakterija mleène kiseline (BMK). Kao selektivna podloga korišæene su: GM17 podloga za laktokoke, MRS podloga za laktobacile i MSE podloga za leukonostoke. Na osnovu mikrobioloških i fizioloških testova izolati su identifikovani do nivoa vrste. Vrste Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides i Enterococcus faecium izolovane su iz uzoraka mleka i sira, dok su vrste Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus brevis i Eterococcus fecalis izolovane samo iz sira. Korišæenjem mikrobioloških i fizioloških testova 20% izolata nije bilo moguæe identifikovati. Analiza plazmidnog profila i ispitivanje proteolitièke aktivnosti uraðeno je za 73 odabrana izolata. Veæina izolata Lactococcus lactis subsp. lactis kao i homofermentativni laktobacili pokazuju dobru proteolitièku aktivnost, dok su izolati iz rodova Leuconostoc i Enterococcus slabi proteoliti. Plazmidi su detektovani kod veæine izolata i plazmidni profili pokazuju varijabilnost. ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM RADAN'S CHEESE In this study we described characterization of 118 natural isolates of lactic acid bacteria (LAB) from cow's milk, ewe's milk and cheese (samples were taken after 5, 10, 20, 30 and 60 days of ripening). The isolates were selected using selective media for LAB, GM17 for lactococci, MRS for lactobacilli and MSE for leuconostoc. Identification of strains to the species level was performed by the analysis of their carbohydrate fermentation pattern and by their physiological characteristics. Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides and Enterococcus faecium were found in milk and cheese, while Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus brevis and Enterococcus faecalis were found only in cheese samples. Twenty percent of isolates were not determinated by used tests. Isolation of plasmids and examination of proteolytic activity were done for 73 isolates. The most strains of Lactococcus lacts subsp. lactis and homofermentative lactobacilli exhibited high proteolytic activity towards b-casein, while strains from genus Leuconostoc and Enterococcus showed weak proteolytic activity. The analysis of plasmid profiles revealed that majority of strains containe different plasmids.
I-Pos-6 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 27 Subotica, 30. novembar - 4. decembar 2004. INVERZIONI POLIMORFIZAM PRIRODNIH POPULACIJA Drosophila subobscura SA LOKALITETA GORNJEG DELA TOKA DUNAVSKOG REGIONA U SRBIJI P. Kalajdiæ 1 , G. ivanoviæ 2 i M. Anðelkoviæ 1,2 1 Biološki fakultet, Univerzitet u Beogradu, Beograd 2 Institut za biološka istraivanja «Siniša Stankoviæ», Beograd Drosophila subobscura poseduje bogat inverzioni polimorfizam na svih pet dugih akrocentriènih hromozoma. Prirodne populacije ove vrste pokazuju prostornu varijabilnost inverzionog polimorfizma koja je u izvesnoj korelaciji sa ekološkim faktorima staništa. Balkansko poluostrvo karakteriše specifièan ekološki diverzitet, naroèito u regionima sa specifiènim mikroklimatskim uslovima kao što je priobalje reènog toka Dunava. Podaci dobijeni analizom inverzionog polimorfizma prirodnih populacija Drosophila subobscura sa lokaliteta gornjeg dela toka Dunavskog regiona omoguæavaju dodatni uvid u raznovrsnost ovog vida genetièkog polimorfizma u ekološkim staništima u regionu sa bogatim biodiverzitetom. CHROMOSOMAL INVERSION POLYMORPHISM OF Drosophila subobscura NATURAL POPULATIONS FROM UPPER PART OF THE DANUBE RIVER REGION IN SERBIA Drosophila subobscura possesses rich inversion polymorphism on all of its five long acrocentric chromosomes. Natural populations of this species shows clear-cut geographical variation of inversion polymorphism, in a certain degree associated with the variety and dynamics of ecological factors. Balkan Peninsula is characterized by specific ecological diversity, especially in specific microclimate regions like the Danube river region. The results derived, by analysis of inversion polymorphism of Drosophila subobscura natural populations from upper part of the Danube river region, are adding more information about the variety of this kind of genetic polymorfism in habitats of region with rich biodiversity.
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Zbornik - Društvo genetičara Srbije

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