Zbornik - Društvo genetičara Srbije

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24 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE I-Pos-3 Subotica, 30. novembar - 4. decembar 2004. ISPITIVANJE GENOTOKSIÈNIH EFEKATA UZORAKA REÈNE VODE ALLIUM ANAFAZNO-TELOFAZNIM TESTOM Jelena Blagojeviæ, Tanja Anaðeviæ, Aleksandra Popoviæ i M. Vujoševiæ Institut za biološka istraiivanja «Siniša Stankoviæ», Beograd Allium anafazno-telofazni test je veoma pogodan test sistem za ispitivanje uzoraka vode (èesmenske vode, reène, jezerske i otpadnih voda) zato što se obavlja bez koncentrisanja uzoraka i ujedno omoguæava praæenje toksiènosti i genotoksiènosti. Za test toksiènosti prati se rast korena, a za test genotoksiènosti registruju se sve promene vezane za hromozome i vreteno tokom anafaze i telofaze. Ovim testom analizirana je potencijalna genotoksiènost uzoraka vode 5 reka u Srbiji: Dunav, Sava, Tamiš, Topèiderska i Borska reka. Iz Dunava su uzeta dva uzorka, kod Slankamena i kod Beograda. Svi uzorci pokazali su slièan nivo genotoksiènosti, koji je znaèajno viši od negativne kontrole, ali još uvek znaèajno nii od pozitivne kontrole. Samo uzorak vode Dunava kod Slankamena ne razlikuje se znaèajno od negativne kontrole, meðutim kada se uporedi sa uzorkom vode Dunava kod Beograda vidi se jasno poveæanje nivoa aberacija ( 2 (1) =17.92, p
I-Pos-4 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 25 Subotica, 30. novembar - 4. decembar 2004. AFLP ANALIZA VARIJABILNOSTI LOKALNIH POPULACIJA KUKURUZA D. Ignjatoviæ-Miciæ, K. Markoviæ i V. Laziæ-Janèiæ Institut za kukuruz «Zemun Polje», Beograd-Zemun Karakterizacija genetièkog diverziteta lokalnih populacija kukuruza u Banci gena Instituta za kukuruz «Zemun Polje» trebalo bi da optimizuje odravanje postojeæe kolekcije i olakša korišæenje datih genotipova. Primena molekularnih markera omoguæava procenu genetièkog diverziteta direktno na DNK nivou prevazilazeæi nedostatke morfoloških i biohemijskih markera. Devetnaest lokalnih populacija kukuruza je analizirano korišæenjem èetiri AFLP prajmer kombinacije. Primenjena je metoda grupnih uzoraka, pri èemu je svaka ispitivana populacija predstavljena sa po 30 biljaka. Nakon denaturišuæe poliakrilamidne elektroforeze gelovi su obojeni srebro-nitratom. Statistièka analiza podataka je uraðena pomoæu NTSYSpc2 programskog paketa, a genetièka distanca je izraèunata primenom akardovog koeficijenta na osnovu binarnog zapisa (1/0) prisustva/odsustva traka kod ispitivanih populacija. Rezultati i diskusija æe biti prikazani na posteru. AFLP ANALYSIS OF MAIZE LOCAL POPULATION VARIABILITY Genetic diversity characterisation of local maize populations from Maize Research Institute «Zemun Polje» genebank should optimize and facilitate their conservation and use. Molecular markers allow the estimation of genetic diversity directly at DNA level, thus overcoming the demerits of morphological and biochemical markers. Nineteen local maize populations were analysed with four AFLP primer combinations. Thirty individual plants were bulked to form DNA pooled samples for each population. Silver staining was applied after the denaturating polyacrilamide electrophoresis. Statistical analysis was performed using NTSYSpc2 programe package and genetic distances were estimated by Jaccard coefficient based on binary codes (1/0) of the bands for their presence/absence within each population. Results and discussion will be presented on the poster.
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Zbornik - Društvo genetičara Srbije

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