Zbornik - Društvo genetičara Srbije

More documents

Recommendations

Info

22 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE I-Pos-1 Subotica, 30. novembar - 4. decembar 2004. EX SITU KONZERVACIJA GENETIÈKIH RESURSA POLJSKOG BRESTA (Ulmus minor Mill.) I VEZA (U. laevis Pall.) Jelena Aleksiæ i S. Orloviæ Univerzitet u Novom Sadu, Poljoprivredni Fakultet, Institut za nizijsko šumarstvo i ivotnu sredinu, Novi Sad Cilj našeg istraivanja je ex situ konzervacija genetièkih resursa poljskog bresta (Ulmus minor Mill.) i veza (U. laevis Pall.) putem osnivanja poljskih banaka gena. Obe vrste su osetljive na Holandsku bolest brestova (DED) koju izaziva gljiva Opiostoma novo-ulmi Brasier, iako je druga vrsta više ugroena narušavanjem prirodnih staništa. Uzorkovanje je sprovedeno u po jednoj populaciji poljskog bresta i veza. Navedena populacija poljskog bresta, pored pojedinaènih, nasumièno rasporeðenih stabala, predstavlja jedinu grupu stabala na podruèju Gazdinske Jedinice Vinièna-eravinac-Puk (ukupna površina 3.552,81ha). Populacija veza se nalazi na površini od 2,43ha i predstavlja jedinu populaciju veza, pored pojedinaènih, nasumièno rasporeðenih stabala, na podruèju Gazdinske jedinice Palanaèke ade-Èipski poloj (ukupna površina 1.283,03ha). Biljni materijal (pupoljci) sa 9 stabala poljskog bresta i 10 stabala veza je iskorišæen za vegetativnu in vitro propagaciju. Dobijeni klonovi su iskorišæeni za osnivanje poljskih banaka gena na oglednom dobru Instituta za nizijsko šumarstvo i ivotnu sredinu. Moguænost naknadne infekcije fungalnim patogenom æe se izbeæi sadnjom klonova na manjem rastojanju od uobièajenog, pošto je pokazano da tako saðena stabla nisu atraktivna za brestove potkornjake (Scolytus sp.) koji su vektori bolesti. Rezutati istraivanja su pokazali da su in vitro tehnike pogodne za masovnu proizvodnju klonova obe vrste, kao i da se osnivanjem poljskih banaka gena moe uspešno realizovati ex situ konzervacija genetièkih resursa navedenih vrsta. EX SITU CONSERVATION OF GENETIC RESOURCES OF FIELD ELM (Ulmus minor Mill.) AND EUROPEAN WHITE ELM (U. laevis Pall.) The aim of our research is ex situ conservation of genetic resources of field elm (Ulmus minor Mill.) and European white elm (U. laevis Pall.) through establishment of field genebank. Both species are susceptible to Dutch Elm Disease (DED) caused by the vascular wilt fungus Ophistoma novo-ulmi Brasier, although the later one is more seriously threatened by the destruction of the natural habitat. Sampling was conducted in one population of field elm and white elm, respectively. Population of field elm represents the only one cluster of elm trees, besides solitary, randomly spread trees, in whole Management Unit Vinièna-eravinac-Puk (total area 3.552,81ha). Population of white elm is spread on 2,43ha and represents the only one population of elms, besides solitary, randomly spread trees, in whole Management Unit Palanaèke ade-Èipski poloj (total area 1.283,03ha). Plant material (buds) from 9 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Clones were used for establishment of field genebank at Institute’s of lowland forestry and environment experimental estate. The possibility of further infection with DED was pass over by planting clones in low hedges, which are unattractive for the vector of the fungal pathogen – elm bark beetle (Scolytus sp.). Results pointed out that in vitro techniques are suitable for production of clones in short time for both elm species and that is possible to preserve elm genetic resources through establishment of field genebank.
I-Pos-2 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 23 Subotica, 30. novembar - 4. decembar 2004. ANTIGENOTOKSIÈNI EFEKAT TERPENA IZ ALFIJE T. Beriæ-Bjedov, J. Kneeviæ-Vukèeviæ, B. Vukoviæ-Gaèiæ, G. Joksiæ 1 , D. Mitiæ-Æulafiæ, B. Nikoliæ, J. Stanojeviæ, S. Stankoviæ, O. Stajkoviæ i D. Simiæ Katedra za mikrobiologiju, Biološki fakultet, Univerzitet u Beogradu, Beograd 1 Institut za nuklearne nauke «Vinèa», Beograd Istraivanja su pokazala da aktivne supstance lekovitih i aromatiènih biljaka poseduju inhibitorni ili modulatorni efekat na genotoksiène agense. Cilj rada je detekcija antigenotoksiènog efekta terpena iz etarskog ulja alfije i izuèavanje mehanizama reparacije DNK koji su ukljuèeni, pomoæu prokariotskih i eukariotskih model sistema. Korišæena je baterija testova na E. coli K12 kojom se prati efekat na UV-indukovanu mutagenezu u reparaciono sposobnom, i eksciziono defektnom soju; spontane mutacije; SOS indukcija i intrahromozomalna rekombinacija. Kao dodatak, uraðen je mikronukleus test na humanim limfocitima ozraèenim zracima. Etarsko ulja alfije ima znatno manji efekat na smanjenje broja UV-indukovanih mutanata u uvrA - soju, nego u eksciziono sposobnom soju. Takav efekat je naðen i kod svih testiranih monoterpena iz njegovog sastava (tujon, 1,8-cineol i kamfor). U mutator mutS soju, nije uoèen znaèajan efekat na broj spontanih mutanata. tujon inhibira UV-indukovani SOS odgovor. Etarsko ulje alfije, 1,8-cineol i kamfor blago stimulišu intrahromozomalnu rekombinaciju u ozraèenom recA + soju, dok tujon nema znaèajnog efekta. Mikronukleus test je pokazao da etarsko ulje alfije ima slab radioprotektivni efekat. Rezultati dobijeni u ovom istraivanju, kao i podaci o antimutagenoj i antikancerogenoj aktivnosti monoterpena, ohrabruju dalja istraivanja etarskog ulja alfije kao potencijalnog agensa za prevenciju kancera. ANTIGENOTOXIC EFFECT OF TERPENOIDS FROM SAGE It has been shown that active substances from medicinal and aromatic plants possess inhibiting or modulating effect on environmental genotoxic agenta. This work is designed to detect antigenotoxic effects of terpenes from essential oil of sage, and to study the DNA repair mechanisms involved, using pro- and eukaryotic model systems. The battery of tests on E. coli K12 measuring UV-induced mutagenesis in excision repair proficient and deficient strains, spontaneous mutations, SOS induction and intrachromosomal recombination was used. In addition, micronucleus test was performed in -irradiated human limphocytes. Essential oil of sage was significantly less effective in inhibition of UV-induced mutations in uvrA - strain than in excision repair proficient strain. Similar effect is observed with all tested monoterpenes from essential oil ( thujone, 1,8-cineole and camphor). No significant effect on spontaneous mutagenesis was found in mutator mutS strain. thujone inhibit UV-induced SOS induction. Essential oil of sage, cineole and camphor mildly stimulate intrachromosomal recombination in UV-irradiated recA + strain, while thujone has no significant effect. Essential oil of sage exhibits mild radioprotective effect in micronucleus test. Our results, as well as the data on antimutagenic and anticarcinogenic activity of monoterpenes, encourage further investigation of essential oil of sage for potential use as cancer preventing agent.
Page 1 and 2: DRUŠTVO GENETIČARA SRBIJE SERBIAN
Page 3 and 4: III KONGRES GENETIÈARA SRBIJE Subo
Page 5 and 6: Uvodno predavanje Opening lecture
Page 7 and 8: I tematska oblast / I topic: Geneti
Page 9: I-Uvo-2 ZBORNIK ABSTRAKATA III KONG
Page 12 and 13: 12 ZBORNIK ABSTRAKATA III KONGRESA
Page 43 and 44: II tematska oblast / II topic: Stru
Page 45 and 46: II-Uvo-2 ZBORNIK ABSTRAKATA III KON
Page 49 and 50: II-Usm-2 ZBORNIK ABSTRAKATA III KON
Page 57 and 58: II-Pos-2 ZBORNIK ABSTRAKATA III KON
Page 63 and 64: III tematska oblast / III topic: Ge
Page 65: III-Uvo-2 ZBORNIK ABSTRAKATA III KO
Page 72 and 73:
72 ZBORNIK ABSTRAKATA III KONGRESA
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 89 and 90:
IV tematska oblast / IV topic: Ople
Page 91 and 92:
IV-Usm-2 ZBORNIK ABSTRAKATA III KON
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
IV-Usm-10 ZBORNIK ABSTRAKATA III KO
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
IV tematska oblast / IV topic: Ople
Page 119 and 120:
IV-Pos-2 ZBORNIK ABSTRAKATA III KON
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
IV-Pos-10 ZBORNIK ABSTRAKATA III KO
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
V tematska oblast / V topic: Geneti
Page 181:
V-Uvo-2 ZBORNIK ABSTRAKATA III KONG
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 203 and 204:
V tematska oblast / V topic: Geneti
Page 205 and 206:
V-Pos-2 ZBORNIK ABSTRAKATA III KONG
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
V-Pos-10 ZBORNIK ABSTRAKATA III KON
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
ZAVRŠNO PREDAVANJE CLOSING LECTURE
Page 229 and 230:
A Aksentijeviæ I. 194 Aleksiæ J.
Page 231 and 232:
Kovaèev L. 73, 90, 125, 140, 147 K
Page 233 and 234:
Stanojeviæ B. 223 Stanojeviæ J. 2
show all

Zbornik - Društvo genetičara Srbije

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?