Zbornik - Društvo genetičara Srbije

226 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE V-Pos-23
Subotica, 30. novembar - 4. decembar 2004.
KORELACIJA INFEKCIJE VISOKORIZIÈNIM HUMANIM
PAPILOMA VIRUSIMA SA TOKOM BOLESTI U BOLESNIKA SA ORALNIM
KARCINOMU USNE DUPLJE
Z. Magiæ 1 , N. Joviæ 2 , R. Kozomara 2 i J. Stojanoviæ 1
1
Institut za medicinska istraivanja, Vojnomedicinska akademija, Beograd
2
Klinika za maksilofacijalnu hirurgiju, Vojnomedicinska akademija, Beograd
Analizirano je 50 pacijenata u II i III stadijumu OSCC jezika i poda usne duplje
(histološki i nuklearni gradus 1, 2 i 3). Starost pacijenata se kretala od 45 do 72 godine,
97% su bili pušaèi dok je 93% konzumiralo alkohol. DNK iz tumorskog tkiva je
izolovana fenol-hloroformskom ekstrakcijom. Prisustvo visokoriziènih tipova HPV (16,
18, 31 i 33) je detktovano PCR-om i PAGE-om. HPV infekcija je detktovana kod 64%
pacijenata. HPV16 je detektovan kod 10 pacijenata (31.2%), HPV18 i HPV31 kod 6
(18.7%), dok HPV33 nije detektovan. Kod 3 pacijenta (9.3%) su detektovani
istovremeno HPV16 i HPV31 a HPV18 i HPV31 kod 7 (21%). Tumori u III klinièkom
stadijumu su u 58% sluèajeva sadrali neki od ispitivanih virusa. Umereno-diferentovani
OSCC sa srednjim nuklearnim gradusom su najèešæe bili inficirani sa HPV (46.8%),
dobro diferentovani 40.6%, a slabo-difrentovani svega 12.5%. Distribucija HPV
infekcije u tumorima niskog nuklearnog gradusa (28.1%) i visokog nuklearnog gradusa
(25%) je bila slièna. Pušenje i konzumacija alkohola su zavisni prediktivni faktor HPV
infekcije. Period bez pojave bolesti (DFI) u ispitivanoj grupi pacijenata se kretao od 4 do
36 meseci. DFI i ukupno preivljavanje (OS) kod pacijenata sa HPV infekcijom su bili
znaèajno kraæi u odnosu na pacijente bez HPV infekcije (log rank test). Karcinomi usne
duplje su èesto inficirani kancerogenim tipovima HPV. U ispitivanoj grupi pacijenata
napena je znaèajna povezanost infekcije tumora kancerogenim tipovima HPV sa tokom i
prognozom bolesti.
CORRELATION OF HIGH RISK HUMAN PAPILLOMAVIRUS INFECTION
WITH DISEASE PROGRESSION IN PATIENTS WITH ORAL
SQUAMOCELLULAR CARCINOMA
We analysed 50 patients with stage II and III of hystologically confirmed OSCC of the
tonguae or oral floor (hystological and nucler grade 1, 2 or 3). Patients were 45-72 years
old, 97% were heavy smokers, 93% were alcochol consumers. Genomicic DNA was isolated
according to standard procedure with phenol/chloroform/isoamilalcohol. Presence
of HPV types 16, 18, 31 and 33 was detected by PCR/PAGE. HPV infection was detected
in 64% of patients. HPV16 was found in 10 pts (31.2%), HPV18 and HPV31 in 6
(18.7%) and none with HPV33. Double positeve HPV infection HPV16+31 was detected
in 3 patients (9.3%) and HPV18+31 in 7 patients (21%). Tumors in clinical stage III was
more likely to contain HPV (58%). Moderately-differentiated OSCC with medium-nuclear
grade were more likely to contain HPV (46.8%), well-differentiated to (40.6%) and
poorly- differentiated in only (12.5%). Low-nuclear grade was detected in 28.1% and
high-nuclear grade in 25% HPV positive tumors. Tobaco usage and alcochol consumption
were an dependent predictor of HPV risk for OSCC. Disease-free interval (DFI) of
patients ranged from 4 to 36 months. DFI and overall survival (OS) in pts with HPV infection
compared to the pts without HPV infection was significantly shorter (log rank
test). Infection with oncogenic HPV is frequent in oral cancers. Viral oncogene expression
and viral integration suggest firm correlation between HPV infection and clinical
course of disease in the examined grouop of patients.