Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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V-Pos-12 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 215<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
PCR DETEKCIJA GENOMA RAZLIÈITIH MIKROORGANIZAMA U<br />
SINOVIJI BOLESNIKA SA REITER-OVIM SINDROMOM TRETIRANIH<br />
AZITROMICINOM<br />
N. Streliæ 1 , Lj. Pavlica 2 i Z. Magiæ 1<br />
1<br />
Institut za medicinska istraivanja, Beograd<br />
2<br />
Klinika za reumatologiju, Vojnomedicinska akademija, Beograd<br />
Bakterijska infekcija zgloba je ozbiljan problem èiji ishod zavisi od pravilnog tretmana.<br />
Mali broj intra-artikularnih bakterija se moe detektovati ultraosetljivim molekularnim<br />
tehnikama, baziranim na lanèanoj reakciji polimeraze (PCR). Brza identifikacija<br />
bakterijske infekcije je neophodna za odgovarajuæi tretman inficiranih pacijenata.<br />
Cilj ovog istraivanja je bio detekcija bakterijske DNK u tkivu sinovije, sinovijskoj<br />
teènosti i perifernoj krvi bolesnika sa Reiter-ovim sindromom tretiranih azitromicinom.<br />
Tkivo sinovije, sinovijska teènost i mononukleari periferne krvi su korišæeni kao izvori<br />
DNK u PCR amplifikaciji bakterijskog 16 S rRNK gena. PCR prajmeri za specifiènu<br />
amplifikaciju DNK fragmenta 16S rRNK gena razlièitih bakterija (Chlamydia<br />
trachomatis, C. pneumoniae, Mycoplasma hominis, Ureaplasma urealyticum) su bili<br />
visoko specifièni. Uzorci su dobijeni od 20 bolesnika sa Reiter-ovim sindromom. DNK<br />
je izolovana fenol-hloroform ekstrakcijom i posle PCR amplifikacije bakterijska DNK je<br />
detektovana na 10% PAGE.<br />
Bakterijska DNK je detektovana u tri uzorka sinovijske teènosti (15%), 11 uzoraka tkiva<br />
sinovije (55%) i 17 uzoraka periferne krvi (80%). Nakon tretmana azitromicinom,<br />
bakterijska DNK je detektovana u tri uzorka sinovijske teènosti (15%), 7 uzoraka tkiva<br />
sinovije (35%) i 10 uzoraka periferne krvi (50%).<br />
Najbolji rezultat terapije azitromicinom je dobijen u krvi posebno za Mycoplasma<br />
hominis.<br />
PCR DETECTION OF GENOME OF DIFFERENT MICROORGANISMS IN<br />
SYNOVIA OF PATIENTS WITH REITER’S SYNDROME TREATED WITH<br />
AZITHROMYCIN<br />
Bacterial joint infection is a serious problem, the outcome of which depends on appropriate<br />
treatment. A small number of intra-articular bacteria can only be detected by using<br />
ultrasensitive molecular techniques, mainly those based on the polymerase chain reaction<br />
(PCR). The rapid identification of bacterial infection is essential for the proper treatment<br />
of infected patients.<br />
The aim of this study was to detect bacterial DNA in synovial tissue, synovial fluid and<br />
peripheral blood of patients with Reiter's syndrome treated with azithromycin.<br />
Synovial tissue, synovial fluid and peripheral blood mononuclear cells were used as the<br />
source of DNA for PCR amplification of bacterial 16 S rRNA gene. PCR primers for the<br />
specific amplification of DNA fragment of the 16 S rRNA gene of different bacteria<br />
(Chlamydia trachomatis, C. pneumoniae, Mycoplasma hominis, Ureaplasma urealyticum)<br />
were highly specific. Samples were obtained from 20 patients with Reiter’s syndrome.<br />
DNA was isolated by phenol/chloroform extraction and after PCR amplification<br />
bacterial DNA was detected by 10% PAGE.<br />
Bacterial DNA was detected in 3 samples of the synovial fluid (15%), 11 samples of the<br />
synovial tissue (55%), and 17 samples of peripheral blood (80%). After the treatment<br />
with azithromycin, bacterial DNA was detected in 3 samples of the synovial fluid (15%),<br />
7 samples of the synovial tissue (35%), and 10 samples of peripheral blood (50%).<br />
The best result with azithromycin therapy was obtained in blood especially for<br />
Mycoplasma hominis.