Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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V-Usm-16 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 199<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
IN VITRO ANALIZA MIKRONUKLEUSA I ÆELIJSKE KINETIKE U<br />
HUMANIM LIMFOCITIMA POD DEJSTVOM ADRENALINA<br />
Biljana Markoviæ i N. Ðeliæ<br />
Katedra za biologiju, Fakultet veterinarske medicine, Univerzitet u Beogradu, Beograd<br />
Upotrebljeno je osam razlièitih koncentracija adrenalina (opseg 5×10-10 M do 5×10-5 M),<br />
od one koja odgovara fiziološkom nivou ovog hormona u plazmi, preko koncentracija<br />
uporedljivih sa minimalnim, srednjim i maksimalnim terapijskim dozama u humanoj<br />
medicini, sve to 10, 30 i 100 puta jaèih koncentracija od terapijskih doza u humanoj<br />
medicini. Eksperimentalna procedura u potpunosti je odgovarala smernicama OECD za<br />
in vitro mikronukleus test. Upotrebljena je krv tri zdrave muške osobe mlaðe od 35 god.<br />
Statistièka analiza dobijenih rezultata uraðena je Studentovim t-testom i<br />
2 testom.<br />
Dobijeni rezultati ukazuju da adrenalin znaèajno smanjuje kinetiku proliferacije humanih<br />
limfocita praæenu CBPI indeksom (engl. cytochalasine block proliferation index) pri<br />
koncentraciji uporedljivoj sa maksimalnom terapijskom dozom, kao i pri svim višim<br />
koncentracijama adrenalina. Smanjena æelijska kinetika pod uticajem adrenalina u skladu<br />
je sa literaturnim podacima da poveæana kolièina cAMP moe da uspori deobu æelija. S<br />
druge strane, u svim primenjenim koncentracijama adrenalin nije doveo do statistièki<br />
znaèajnog porasta uèestalosti mikronukleusa u humanim limfocitima. Odsustvo<br />
aneugenih i klastogenih efekata moguæe je objasniti ili nedovoljnim metabolièkim<br />
kapacitetom limfocita za konverziju kateholamina, ili nedovoljnom senzitivnošæu samog<br />
mikronukleus testa.<br />
IN VITRO ANALYSIS OF MICRONUCLEI AND CELL CYCLE KINETICS IN<br />
HUMAN LYMPHOCYTES EXPOSED TO EPINEPRHINE<br />
We used eight experimental concentrations of epinephrine in a range from 5×10 -10 Mto<br />
5×10 -5 M, starting with a concentration which corresponds to physiological level of this<br />
hormone in human plasma, through concentrations corresponding to minimal, average<br />
and maxmial therapeutic doses in human medicine, and finally 10-, 30- and 100-fold<br />
max. therapeutic doses. The experimental procedure was entirely according to OECD in<br />
vitro micronucleus assay guiedlines. Three healthy men under 35 donated blood for these<br />
experiments. Statistical analysis was performed by Student´s t-test and 2 test.<br />
Epinephrine significantly decreased progression through the cell cycle measured by<br />
CBPI (cytochalasine block proliferation index) at the concentration comparable to maximal<br />
therapeutic dose, as well as all higher concentrations. Cell cycle delay is consistent to<br />
literature data that increased level of cAMP can supress mitotic activity. On the other<br />
hand, there is no significant change in micronucleus frequencies at all concentrations<br />
analysed. The abscence of aneugenic and clastogenic effects may have resulted from<br />
poor metabolic capacity of human lymphocytes, or undersensitivity of micronucleus assay<br />
itself.