Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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184 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE V-Usm-1<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
ANALIZA KLONALNOSTI T-ÆELIJA U TUMORSKIM UZORCIMA<br />
BOLESNICA SA KARCINOMOM DOJKE<br />
Bojana Cikota 1 , Mirjana Brankoviæ-Magiæ 2 i Z. Magiæ 1<br />
1<br />
Institut za medicinska istraivanja, Vojnomedicinska akademija, Beograd<br />
2<br />
Institut za onkologiju i radiologiju <strong>Srbije</strong>, Beograd<br />
Rezultati brojnih istraivanja su pokazali da razlièiti tumori, ukljuèujuæi i tumore dojke,<br />
mogu stimulisati specifièni T-æelijski antitumorski odgovor. Prepoznavanje tumor-specifiènog<br />
antigena (kojeg tumorska æelija eksprimira endogeno ili egzogeno kao<br />
peptid prezentovan putem antigen-prezentujuæih æelija) rezultuje klonalnom ekspanzijom<br />
i diferencijacijom naivnih CD8 + T-æelija u aktivirane efektorske æelije koje uèestvuju u<br />
ubijanju tumorskih æelija. U ovom istraivanju je analizirana klonalnost T-æelija u<br />
tumorskim uzorcima 54 bolesnice sa karcinomom dojke. Na osnovu prisustva/odsustva<br />
metastaza u regionalnim limfnim èvorovima bolesnice su klasifikovane u grupu N+<br />
(prisutne metastaze; n=25) ili N0 (bez metastaza; n=16). Analiza klonalnosti T-æelija se<br />
zasnivala na PCR amplifikaciji dela rearaniranog gena za lanac TCR-a i PAGE.<br />
Prisustvo monoklonalne/oligoklonalne (M/O) populacije T-æelija je detektovano u 9<br />
bolesnica iz N+ i 6 bolesnica iz N0 grupe. U svim analiziranim grupama (N + +N0, N+,<br />
N0) nije uoèena statistièki znaèajna razlika u uèestalosti relapsa izmeðu bolesnica sa M/O<br />
u odnosu na bolesnice sa poliklonskom T-æelijskom populacijom. Poreðenjem duine<br />
perioda bez bolesti (DFI) tokom prve dve godine u bolesnica sa M/O T-æelijama u<br />
odnosu na bolesnice sa poliklonskom populacijom je dobijena statistièki znaèajna razlika<br />
graniène vrednosti samo u N+ grupi. Bolesnice sa M/O T-æelijama su imale kraæi DFI od<br />
bolesnica sa poliklonskom populacijom. Ova razlika nije uoèena kada se analiza<br />
zasnivala na periodu praæenja duem od 2 godine.<br />
Dobijeni rezultati ukazuju da su populacije tumor infiltrirajuæih limfocita uglavnom<br />
poliklonalne.<br />
ANALYSIS OF T-CELL CLONALITY PATTERN IN TUMOR SAMPLES OF<br />
BREAST CANCER PATIENTS<br />
Numerous experimental evidences confirm that different tumors, including breast carcinomas,<br />
can stimulate specific T-cell mediated immune response. Recognition of tumor-associated<br />
antigen (expressed endogenously by tumor cells or exogenously as peptide<br />
presented by antigen-presenting cells) results in the clonal expansion and differentiation<br />
of naive CD8+ T-cells into activated effector cells that mediate tumor cell killing. In<br />
this study we have analyzed pattern of T-cell clonality in tumor samples of 54 breast cancer<br />
patients classified as lymph node-negative – N0 (n=16) or lymph node-positive – N+<br />
(n=25). Analysis of T-cell clonality was based on PCR amplification of rearranged TCR<br />
chain gene followed by PAGE.<br />
Monoclonal/oligoclonal (M/O) T-cell populations were found in 9 of N + and6ofN0 breast cancer patients, respectively. In all analyzed groups (N + +N0, N+, N0), incidence<br />
of relapse was not significantly different between patients with M/O T-cells vs patients<br />
with polyclonal T-cells. Comparison of disease free interval (DFI) between the patients<br />
divided according to the presence of TCRã monoclonality/oligoclonality showed<br />
board-line significant difference only in the group of N+ patients within first 24 months<br />
of follow-up. Patients with M/O T-cell population had shorter DFI than patients with<br />
polyclonal T-cell population. Concerning the complete follow-up in the same group of<br />
patients, this difference was not observed.<br />
Our results imply that tumor infiltrating T-cells are usually polyclonal.