Zbornik - Društvo genetičara Srbije

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172 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-55 Subotica, 30. novembar - 4. decembar 2004. POLIMORFIZAM MOLEKULARNIH MARKERA KOD INBRED LINIJA KUKURUZA I HETEROZIS G. Driniæ, J. Srdiæ. S. Mladenoviæ-Driniæ i K. Konstantinov Institut za kukuruz «Zemun Polje», Beograd U radu je prouèavana genetièka divergentnost inbredovanih linija kukuruza proteinskim i RAPD markerima i korelacija dobijenih podataka sa ispoljenim heterozisom za prinos zrna u njihovim hibridnim kombinacijama. Deset inbredovanih linija, razlièitog porekla i duine vegetacije dialelno su ukrštene i dobijene hibridne kombinacije su ispitane u tri lokacije. Najveæi heterotièni efekat je ispoljila kombinacija ZPPL15 i ZPPL 200. Ukupno je detektovano 42 proteinske frakcije od kojih je 76% polimorfno. Sa RAPD markerima je utvrðen viši nivo polimorfizma (81%). Klaster analiza na osnovu genetièke distance sa oba tipa markera kao i heterotiènog efekta za prinos zrna grupisala je ispitivane genotipove u dve grupe. Grupisanje na osnovu klaster i PCA analize odgovaralo je podacima o njihovom poreklu. Znaèajna korelacija utvrðena je izmeðu genetièke distance roditeljskih linija u njihovim hibridnim kombinacijama i heterozisa za prinos zrna tih hibrida. POLYMORPHISM OF MOLECULAR MARKERS OF MAIZE INBRED LINES AND HETEROSIS The objective of the present study was to evaluate genetic diversity among maize inbred lines using polymorphism of protein and RAPD markers and to correlate single cross heterosis, to the genetic distance of the parental lines. Ten maize inbred lines of different origin and maturity were crossed diallelly without reciprocal crosses and their combinations were teseted at three location. The highest heterosis for yield was detected in the combination ZPPL15 x ZPLL 200. The analysis of embryo salt solubile proteins showed that all studied genotipes have a specific protein pattern. Totally 42 protein fractions of different molecular weight were observed, from which 76% of protein fractions were polymorphic.With RAPD markers higer polymorphism was detected (81%). The cluster analysis based on genetic distance computed from protein and RAPD data as well as heterosis classifies each of 10 inbreds into two groups. It could be concluded from the present study that grouping of inbred lines by Claster and PCA generally agree with the pedigrees of these lines. The correlation coefficient between heterosis for grain yield and genetic distances based on molecular markers are positive and mainly significant.
IV-Pos-56 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 173 Subotica, 30. novembar - 4. decembar 2004. EFEKAT MUTANATA ENDOSPERMA NA KLIJAVOST SEMENA KUKURUZA Zorica Pajiæ, Lana Ðukanoviæ i U. Eriæ Institut za kukuruz «Zemun Polje», Zemun Ispoljavanje genetièkog potencijala rodnosti i kvaliteta odreðenog genotipa zavisi, pored drugih faktora, i od kvaliteta semena. Seme je veoma vano kako za reprodukciju odreðene biljne vrste tako i za savremenu biljnu proizvodnju. Svaki od delova semena kukuruza, perikarp, endosperm i klica, ima odreðenu funkciju u kompleksnom procesu klijanja i nicanja. Ispitivana su tri genotipa razlièitog tipa endosperma: ZPSC 42A, hibrid standardnog kvaliteta zrna (zuban), ZPSC 504 su (hibrid šeæerca sa sugary genom) i ZPSyn.II sh2 (sintetièka populacija sa shrunken2 genom). Za odreðivanje vitalnosti semena navedenih genotipa korišæene su priznate ISTA metode: standardni metod, ubrzano starenje i cold test. Rezultati pokazuju da postoje razlike u kapacitetu klijanja ispitivanih genotipova. Najveæe smanjenje kapaciteta klijavosti i brzine nicanja ispoljeno je primenom metode ubrzanog starenja. Nastale razlike su verovatno posledica graðe (tipa) endosperma, teine zrna, sadraja šeæera i debljine i sastava perikarpa. EFFECT OF ENDOSPERM MUTANTS ON MAIZE SEED GERMINATION The expression of genetic potential of yielding and quality of a certain genotype depends among other factors on seed quality. Seed is very important not only for the reproduction of the particular plant species, but also, for the contemporary plant production. Each part of maize seed (pericarp, endosperm and germ) has a specific function in the complex process of germination and emergence. The following three genotypes of different endosperm types were observed: ZPSC 42A (standard grain quality dent hybrid), ZPSC 504 su (sweet corn hybrid with a sugary gene) and ZPSyn.II sh2 (synthetic population with a shrunken2 gene). Seed vitality of the stated genotypes was determined by the accepted ISTA methods: standard method, accelerating age and cold test. Obtained results point out to differences in the germination capacity of the observed genotypes. The greatest reduction of the germination capacity and the emergence rate was expressed by the application of the accelerating ageing method. Appeared differences are probably a result of the endosperm texture (type), grain weight, sugar content and pericarp thickens and composition.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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