Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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172 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-55<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
POLIMORFIZAM MOLEKULARNIH MARKERA KOD INBRED<br />
LINIJA KUKURUZA I HETEROZIS<br />
G. Driniæ, J. Srdiæ. S. Mladenoviæ-Driniæ i K. Konstantinov<br />
Institut za kukuruz «Zemun Polje», Beograd<br />
U radu je prouèavana genetièka divergentnost inbredovanih linija kukuruza proteinskim i<br />
RAPD markerima i korelacija dobijenih podataka sa ispoljenim heterozisom za prinos<br />
zrna u njihovim hibridnim kombinacijama. Deset inbredovanih linija, razlièitog porekla i<br />
duine vegetacije dialelno su ukrštene i dobijene hibridne kombinacije su ispitane u tri<br />
lokacije. Najveæi heterotièni efekat je ispoljila kombinacija ZPPL15 i ZPPL 200. Ukupno<br />
je detektovano 42 proteinske frakcije od kojih je 76% polimorfno. Sa RAPD markerima<br />
je utvrðen viši nivo polimorfizma (81%). Klaster analiza na osnovu genetièke distance sa<br />
oba tipa markera kao i heterotiènog efekta za prinos zrna grupisala je ispitivane<br />
genotipove u dve grupe. Grupisanje na osnovu klaster i PCA analize odgovaralo je<br />
podacima o njihovom poreklu. Znaèajna korelacija utvrðena je izmeðu genetièke distance<br />
roditeljskih linija u njihovim hibridnim kombinacijama i heterozisa za prinos zrna<br />
tih hibrida.<br />
POLYMORPHISM OF MOLECULAR MARKERS OF MAIZE<br />
INBRED LINES AND HETEROSIS<br />
The objective of the present study was to evaluate genetic diversity among maize inbred<br />
lines using polymorphism of protein and RAPD markers and to correlate single cross<br />
heterosis, to the genetic distance of the parental lines. Ten maize inbred lines of different<br />
origin and maturity were crossed diallelly without reciprocal crosses and their combinations<br />
were teseted at three location. The highest heterosis for yield was detected in the<br />
combination ZPPL15 x ZPLL 200. The analysis of embryo salt solubile proteins showed<br />
that all studied genotipes have a specific protein pattern. Totally 42 protein fractions of<br />
different molecular weight were observed, from which 76% of protein fractions were<br />
polymorphic.With RAPD markers higer polymorphism was detected (81%). The cluster<br />
analysis based on genetic distance computed from protein and RAPD data as well as<br />
heterosis classifies each of 10 inbreds into two groups. It could be concluded from the<br />
present study that grouping of inbred lines by Claster and PCA generally agree with the<br />
pedigrees of these lines. The correlation coefficient between heterosis for grain yield and<br />
genetic distances based on molecular markers are positive and mainly significant.