Zbornik - Društvo genetičara Srbije

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16 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE I-Usm-5 Subotica, 30. novembar - 4. decembar 2004. ADAPTIVNOST ILI STABILNOST Amy-GENOTIPOVA Drosophila subobscura U USLOVIMA KONTINUIRANIH I NAGLIH PROMENA KONCENTRACIJE SKROBA U HRANLJIVOM MEDIJUMU Tatjana Saviæ 1 , Marina Stamenkoviæ-Radak 2 i M. Anðelkoviæ 1,2 1 Institut za biološka istraivanja «Siniša Stankoviæ», Beograd 2 Biološki fakultet, Univerziteta u Beogradu, Beograd Analizom fenotipske varijabilnosti Amy-genotipova Drosophila subobscura na razlièitim koncentracijama skroba, kao model-sistema, mogu se razmotriti oblici, pravci i efekti interakcije genotip/sredina. U našim istraivanjima analiziran je uticaj postepenih i naglih promena koncentracije skroba na specifiènu aktivnost – amilaze, duinu razviæa i preivljavanje kod jedinki Drosophila subobscura homozigotnih ili heterozigotnih za «spori» i «brzi» amilazni alel. Proseène vrednosti za specifiènu aktivnost - amilaze, duinu razviæa i preivljavanje u uslovima postepenih i naglih promena koncentracije skroba ukazuju na opštu proseènu genetièku varijabilnost fenotipova koji je pod znaèajnim uticajem sredine (razlièita koncentracija skroba) i posmatrane karakteristike su fenotipski plastiène. Norme reakcija za proseène vrednosti analiziranih karakteristika takoðe ukazuju na postojanje znaèajnog sredinskog uticaja, kao i da je u njihovoj osnovi genetièka varijabilnost jedinki Drosophila subobscura. Rezultati dobijeni na osnovu proseènih indeksa fenotipske plastiènosti za specifiènu aktivnosti -amilaze, duinu razviæa i preivljavanje navode na zakljuèak da jedinke Drosophila subobscura u svakoj sledeæoj generaciji, odnosno sa poveæanjem skroba u hranljivom supstratu, adaptabilno odgovaraju u pogledu preivljavanja, za razliku od specifiène aktivnosti -amilaze i duine razviæa èiji proseèni indeksi fenotipske plastiènosti ukazuju na stabilniji odgovor. ADAPTABILITY OR STABILITY OF Amy-GENOTYPES OF Drosophila subobscura IN DISCRETE AND IMMEDIATE CHANGES OF STARCH CONCENTRATIONS IN NUTRITIVE SUBSTRATE Through the analysis of phenotypic variability of Amy-genotypes of Drosophila subobscura on different starch concentrations, as a model-system, the shapes, directions and effects of genotype/environment interactions can be determined. Our research includes the study of the effect of discrete and immediate changes of starch concentrations on – amylase specific activity, development time and viability of Drosophila subobscura, homozygous or heterozygous for «slow» and «fast» amylase allele. The mean values of – amylase specific activity, development time and viability under discrete and immediate changes of starch concentrations, show that the overall genetic variability of phenotypes is under significant environmental influence (different starch concentrations) and that studied characters show phenotypic plasticity. Norms of reactions for the mean values of analyzed traits also point towards existence of significant environmental influence and individual genetic variability of Drosophila subobscura. The results obtained for the mean indices of phenotypic plasticity for - amylase specific activity, development time and viability, suggest that Drosophila subobscura flies, through each succeeding generation and increased starch concentrations in substrate, show adaptive response regarding viability, but their mean indices of phenotypic plasticity for development time and specific activity of the enzyme indicate the more stable response.
I-Usm-6 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 17 Subotica, 30. novembar - 4. decembar 2004. VARIJABILNOST KOMPOZICIJE Gli-ALELA KOD PŠENICE (Triticum aestivum L.) D. Kneeviæ 1 , Aleksandra Yurievna-Dragovich 2 i Nevena Ðukiæ 3 1 Institut za istraivanja u poljoprivredi SRBIJA, Centar za strna ita, Kragujevac 2 Institut opšte genetike, Moskva, Rusija 3 Prirodno-matematièki fakultet Kragujevac, Institut za biologiju, Kragujevac Analizirana je kompozicija glijadina kod 25 sorti pšenice stvorenih u Kragujevaèkom selekcionom centru. Aleli na Gli-1 i Gli-2 lokusima su identifikovani na bazi blokova komponenti glijadina. U prouèavanjima je identifikovano ukupno 30 glijadinskih alela i to:4naGli-A1, 6naGli-B1, 6naGli-D1, 5naGli-A2, 5naGli-B2 i 4 alela na Gli-D2 lokusu. Uèestalost identifikovanih alela bila je razlièita. Najfrekventnij aleli bili su: Gli-A1a (44,00%), Gli-B1b, (48,00 %), Gli-D1b (56,00 %), Gli-A2b (36,00 %), Gli-B2b (60,00 %), Gli-D2b (60,00 %). Visoka uèestalost verovatno je rezultat ogranièene (suene) germ-plazme korišæene pri ukrštanju a takoðe i visoke adaptivnosti genotipova koji su imale ove genske alele, zbog èega su ih oplemenjivaèi èešæe odabirali u toku procesa oplemenjivanja. VARIABILITY OF Gli-ALELLE COMPOSITION IN WHEAT (Triticum aestivum L.) The gliadin composition in 25 wheat cultivars created in Kragujevac’s breeding Center was analyzed. Alleles at Gli-1 and Gli-2 loci were identified on the base of gliadin block components. The 30 alleles were identified namely: 4 at Gli-A1,6atGli-B1,6atGli-D1, 5atGli-A2, 5 at Gli-B2, 4atGli-D2. Frequency of identified alleles was different. The most frequent alleles have been Gli-A1a (44,00%), Gli-B1b, (48,00 %), Gli-D1b (56,00 %), Gli-A2b (36,00 %), Gli-B2b (60,00 %), Gli-D2b (60,00 %). The high value of frequency is likely results of narrow germplasm which used for cossing and higher adaptability of genotypes that carried those alleles because breeders selected frequently those genotypes during breeding process.
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Zbornik - Društvo genetičara Srbije

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