Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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IV-Pos-52 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 169<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
GENETIÈKA SLIÈNOST GENOTIPOVA SOJE DOBIJENA<br />
NA OSNOVU GENETIÈKIH MARKERA<br />
A. Nikoliæ, M. Srebriæ i S. Mladenoviæ-Driniæ<br />
Institut za kukuruz «Zemun Polje», Beograd-Zemun<br />
Genetièka osnova soje je veoma uska. Tradicionalno, genetièki diverzitet soje procenjivao<br />
se na osnovu morfoloških i agronomskih svojstava ili na informacijama o poreklu.<br />
Cilj ovog istraivanja je bio da se proceni informativnost izabranih genetièkih markera da<br />
bi se utvrdio genetièki diverzitet izmeðu 20 genotipova soje, kao i svrstavanje genotipova<br />
soje u odreðene grupe u zavisnosti od genetièke sliènosti.<br />
U ovom radu korisitli smo morfološke, proteinske i RAPD markereza utvrðivanje<br />
genetièke sliènosti.proteini semena su izolovani i razdvojeni PAA elektroforezom.<br />
Genomska DNK iz ovih genotipova okarakterisana je RAPD markerima korišæenjem 20<br />
izabranih prajmera. Na osnovu prisustva/odsustva traka na proteinskim<br />
gelovima/amplifikovanih DNK fragmenata izraèunati su koeficijenti sliènosti izmeðu<br />
parova genotipova. Za klaster analizu korišæen je UPGMA metod, a za neophodna<br />
proraèunavanja korišæen je NTSYS-pc program.<br />
Prema dobijenim rezultatima, genotipovi soje su svrstani u odgovarajuæe grupe. RAPD<br />
Markeri su detektovali viši nivo polimorfizma nego proteinski markeri. DNK markeri<br />
kao što su RAPD markeri su informativniji u proceni genetièkog diverziteta nego<br />
proteinski markeri.<br />
GENETIC SIMILARITY AMONG SOYBEAN GENOTYPES REVEALED BY<br />
GENETIC MARKERS<br />
The genetic diversity of cultivated soybean is very narrow. Traditionally, its estimation<br />
has been based on the differences in morphological and agronomic traits or pedigree information.<br />
The objective of this research were to evaluate informativeness of chosen genetic markers<br />
for estimating genetic diversity among 20 soybean genotypes, and assigning soybean<br />
genotypes into groups according to genetic similarity.<br />
In this study, we used morphological, protein and RAPD markers to validate their genetic<br />
relationship.Seed proteins were isolated and separated by PAA electrophoresis.Genomic<br />
DNA from these genotypes was characterized by RAPD markers with 20 selected<br />
decamer primers. On the basis of the presence/absence of bands in protein gels/amplified<br />
DNA fragments, coefficients of similarity were calculated between pairs of genotypes.<br />
The similarity matrix was submited for hierarchial cluster analysis of unweighted pair<br />
group using arithmetic average (UPGMA) method and necessary computation were performed<br />
using NTSYS-pc program.<br />
According to obtained results, soybean genotypes were assigned to correspoding groups.<br />
RAPD markers detected hogher level of polymorphism in soybean than protein markers.<br />
DNA markers such as RAPDs are more informative in estimating genetic diversity compared<br />
with protein markers.