Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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IV-Pos-26 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 143<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
UTICAJ DIAZOTROFA NA NEKE FIZIOLOŠKE PARAMETRE PŠENICE<br />
Danica Micanoviæ, Veselinka Zeèeviæ, D. Kneeviæ, M. Pavloviæ i D. Uroševiæ<br />
Institut za istraivanja u poljoprivredi «SRBIJA», Centar za strna ita, Kragujevac<br />
Istraivanja su izvedena u laboratorijskim uslovima sa tri divergentna genotipa pšenice<br />
(DK-1, DK-2, DK-3). Seme je površinski sterilisano, a zatim naklijavano šest dana.<br />
Biljke su inokulisana sa suspenzijom od 0,5 ml sojeva diazotrofa (Kg-10, Kg-11 Kg-67,<br />
Kg-73, Kg-74, Kg-75 i Az-zp) u koncentraciji 10 8 æelija po biljci. Kao kontrola su sluile<br />
neinokulisane biljke. Biljke su gajene u in vitro uslovima 42 dana, u bezazotnom rastvoru<br />
Reid Yorka za pšenicu. Izolati su determinisani molekularno genetièkom metodom PCR<br />
kao nif bakterije. Uticaj inokulacije je praæen preko sadraja suve materije korena i<br />
nadzemnog dela biljke, visine biljke, duine korena kao i sadraja azota u korenu i<br />
nadzemnom delu biljke. Dobijene su statistièki visoko znaèajne razlike u masi suve<br />
materije i sadraju azota, kako u nadzemnom delu tako i u korenu, kod svih genotipova,<br />
dok razlike kod ostalih ispitivanih parametara nisu bile statistièki opravdane.<br />
Najveæa masa suve materije u nadzemnom delu i korenu biljaka dobijena je kod sorte<br />
DK-1 (18.59 mg/biljkci; 10.14 mg/biljci). Najveæi sadraj azota u nadzemnom delu<br />
biljke dobijen je takoðe kod genotipa DK-1 (0.455 mg/biljci), a u korenu kod genotipa<br />
DK-3 (0.170 mg biljka -1 ). Najveæi uticaj na veæinu ispitivanih parametara imali su sojevi<br />
Kg-74 i Kg-75, proseèno za sve genotipove.<br />
INFLUENCE OF DIAZOTROPHS ON PHYSIOLOGICAL<br />
PARAMETERS IN WHEAT<br />
The experiment was done in semi control conditions with three different wheat genotypes<br />
(DK-1, DK-2, DK-3). Grain surface of each cultivars were sterilized and after that<br />
were germinate during six days. Inoculation has been made with seven different strains<br />
(Kg-10, Kg-11 Kg-67, Kg-73, Kg-74, Kg-75 and Az-zp) with 0,5 ml 10 8 cell per seed.<br />
Control plants were grown in tubes without inoculation. Plants were grown in vitro in nitrogen<br />
free solutions, during 42 days. Pure isolates determined by PCR as nif bacteria.<br />
Influence of inoculation was analyzed on the base of value of assessed traits (dry matter<br />
content of above ground part of plant and root, height of plants, length of root, nitrogen<br />
concentration and nitrogen content of above ground part of plant and root). Variability<br />
was found in majority investigation parameters. Influence on inoculation with<br />
diazotrophs indicated very significant differences of dry matter content of above ground<br />
part of plant and root and nitrogen content in above part of plant and root, but differences<br />
in height of plants and length of root were not significant.<br />
The highest content of dry matter of above ground part of plant and root were found in<br />
cultivar DK-1 (18.59 mg plant -1 ; 10.14 mg plant-1 ). The highest nitrogen content of above<br />
ground part of plant else were found in cultivar DK-1 (0.455 mg plant -1 ) but in root in<br />
cultivar DK-3 (0.170 mg plant -1 ). The highest increasing in most investigation parameters<br />
was influenced by strains Kg-74 and Kg-75 in average for all cultivars.