Zbornik - Društvo genetičara Srbije

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140 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-23 Subotica, 30. novembar - 4. decembar 2004. PRIMENA KULTURE CVASTI in vitro U OPLEMENJIVANJU ŠEÆERNE REPE Sneana Mezei, L. Kovaèev, N. Èaèiæ i Nevena Nagl Nauèni institut za ratarstvo i povrtarstvo, Novi Sad Obzirom da kod šeæerne repe (Beta vulgaris L.) nisu razdvojene faze izduivanja aksilarnih pupoljaka od faze umnoavanja, giberelin se dodaje zajedno sa citokininom i neophodnim mikro i makro elementima u podlogu. Veæ u prvoj subkulturi moe da se dobije desetak biljèica koje su spremne za fazu oiljavanja. U fazi mikropropagacije iz eksplantata cvasti dovoljne su koncentracije od svega 0,3 mg/l BA da se dobije zadovoljavajuæa stopa umnoavanja u periodu od nekoliko subkultura. Podloga za oiljavanje šecerne repe ne sadri fitohormone, a poznato je da se rizogeneza sastoji od faze indukcije, inicijacije i elongacije, te se smatra da je de novo inicijacija korena stimulisana postojanjem endogenih auksina i citokinina u optimalnom odnosu u tkivu repe. Na osnovu kombinacionih sposobnosti utvrðenih za potomstva proizvedena iz semena pojedinaènih biljaka dobijenog u slobodnoj oplodnji, odabrani su genotipovi iz zasnovane klonske populacije i oiljeni in vitro. Nakon toga su biljke aklimatizovane u staklari a u hladnoj komori je izvršena fototermalna indukcija. Seme dobijeno od odabranih klonova predstavlja rekurentnu populaciju oprašivaèa sorte Lara. APPLICATION OF INFLORESCENCE in vitro CULTURE IN SUGAR BEET BREEDING Since in sugar beet (Beta vulgaris L.) elongation of axillary buds in not independent from multiplication, gibrerelins are added to multiplication media with citokynines, microand macro- elements. Already in the first subculture few plantlets could be obtained, which could be put on media fro rhizogenesis. For the micropropagation from inflorescence, concentration of 0,3 mg/l BA is enough to obtain satisfying multiplication rate in few subcultivations. There is no need to add phytohormones in rhizogenesis medium, since it was proved that induction, initiation and elongation of sugar beet roots is spontaneous and result of optimal ratio of endogenous auxines and citokynines. According to combining abilities determined for offspring produced from seed of single plants in free pollination, number of genotypes were selected and served as a base for formation of clone population in vitro. The plants were put on rooting medium, after that on acclimatization in the greenhouse and then in cold chamber for phototermal induction. Seed of selected genotypes presents recurent population of pollinator for variety Lara.
IV-Pos-24 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 141 Subotica, 30. novembar - 4. decembar 2004. GENETIÈKA VARIJABILNOST SORTI LUCERKE RAZLIÈITOG GEOGRAFSKOG POREKLA S. Katiæ, V. Mihailoviæ, D. Miliæ, S. Vasiljeviæ i A. Mikiæ Nauèni institut za ratarstvo i povrtarstvo, Novi Sad Cilj rada je bio da se utvrdi genetièka varijabilnost lucerke u i izmeðu sorti razlièitog geografskog porekla. Ogled je voðen od 2002-2004 godine sa 12 sorti lucerke (M. sativa): dve iz Novog Sada, 4 iz Grèke, 5 iz Bolivije, 1 iz Ukrajine i 3 sorte M. varia iz Estonije. Analizom dvogodišnjih rezultata (2003. i 2004), dobijeno je najveæe variranje izmeðu sorti razlièitog geografskog porekla odnosno izmeðu M. sativa i M. varia sorti. Takoðe bilo je veæe variranje izmeðu sorti sa udaljenim poreklom (Srbija - Bolivija). Najmanje variranje zapaeno je izmeðu sorti bliskog geografskog porekla i ekoloških uslova (Srbija – Grèka). Uoèeno je veæe variranje izmeðu sorti razlièitog geografskog i biološkog porekla, nego izmeðu sorti zajednièkog porekla i sliènih ekoloških uslova. Najveæe genetièko variranje zapaeno je kod osobina: prinos suve materije (CVg 27,8 %) i brzina regeneracije (CVg 24,0 %). Najmanje genetièko variranje izmeðu sorti zapaeno je kod udela lišæa (CVg 8,4 %) i sadraja suve materije (CVg 2,4 %). GENETIC VARIABILITY IN LUCERNE CULTIVARS OF DIFFERENT GEOGRAPHIC ORIGIN The aim of this paper was to analyze genetic variability within and between lucerne cultivars of different geografic origin. A trial was conducted during 2002-2004 with 12 M. sativa cultivars: two from Novi Sad, Serbia, four from Greece, five from Bolivia, one from Ukraine and three cultivars of Medicago varia from Estonia. The results of 2003 and 2004 have shown that the greatest variation was between cultivars of different geographic origin, as well as M. sativa and M. varia. There was also a great variation between cultivars of distant origin (Serbia – Bolivia). The smallest variation was found in cultivars of close geographic origin and environmental conditions (Serbia Greece).There was also a greater variation between cultivars of different geographic and biologacal origin than between cultivars of common origin and similar ecological conditions. The greatest genetic variation was found in dry matter yield (CVg 27.8 %) and regeneration rate (CVg 24.0 %). The smallest genetic variation was found in portion of leaves (CVg 8.4 %) and (CVg 2.4 %).
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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III KONGRES GENETIÈARA SRBIJE Subo
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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