Zbornik - Društvo genetičara Srbije

138 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-21
Subotica, 30. novembar - 4. decembar 2004.
VARIJABILNOST PROTEINA U SEMENU I KLIJAVCIMA 40 LINIJA
POLUSRODNIKA CRNOG BORA (Pinus nigra Arn.)
M. Mataruga 1 , V. Isajev 1,2 , Sneana Mladenoviæ-Driniæ 3 ,
Kosana Konstantinov 3 i Vanja Danièiæ 1
1
Šumarski fakultet, Univerzitet u Banja Luci, Bosna i Hercergovina
2
Šumarski fakultet, Beograd
3
Institut za kukuruz «Zemun Polje», Zemun
Istraivanjima je obuhvaæeno 40 linija crnog bora slobodnog oprašivanja koje potièu iz 5
provenijencija: Sutjeska, Višegrad, Tara, Tesliæ i Durmitor. U svakoj provenijenciji
izdvojene su dve populacije i to: 5 linija slobodnog oprašivanja predstavlja populaciju sa
stena i 3 linije slobodnog oprašivanja populaciju na najboljem staništu crnog bora.
Specifiènost ispitivanih provenijencija, populacija, kao i linija slobodnog oprašivanja
dokazana je preko prouèavanja osobina semena, dinamike klijanja i usvajanja vode, te
osobina klijavaca, a potvrðena putem analiza u solima rastvorljivih proteina. Identièan
proteinski sastav u ponavljanjima iste linije slobodnog oprašivanja je dokazan u malom
broju sluèajeva, što govori u prilog velike unutarlinijske varijabilnosti koja moe biti
posledica uticaja oca, kao i heterozigotnosti materinskih stabala. U analizama
proteinskog sastava semena konstatovane su najznaèajnije razlike na nivou provenijencija.
Kod klijavaca starih 9 dana, isklijalih u uslovima indukovane suše i u
standardnim uslovima utvrðene meðulinijske, populacione i provenijeniène razlike.
Korelacionim analizama utvrðeno je postojanje znaèajnih meðuzavisnosti osobina
klijavaca i kasnijeg razvoja sadnica.
VARIABILITY OF THE SALT-SOLUBLE PROTEINS IN SEED AND
SEEDLING OF 40 HALF-SIB LINIES OF AUSTRIAN PINE
The study includes 40 lines of free pollination originating from 5 provenances: Sutjeska,
Višegrad, Tara, Teslic and Durmitor. Two populations were selected from each provenance,
i.e.: 5 lines of free pollination represent the population growing on the cliffs and 3
lines of free pollination represent the population growing at the best site of Austrian pine.
The specificities of the study provenances, populations, and free pollination lines were
proved by the study of seed properties, dynamics of germination and water uptake, and
by the seedling properties and it was confirmed by the analyses in the salt-soluble proteins.
The identical protein composition in the replicates of the same free pollination line
was proved in a small number of cases, which indicates a high intra-line variability which
can be the result of the effect of father, as well as of heterozygosity of mother trees.
While the analyses of protein composition of seed show the most significant provenance
differences, inter-line, population and provenance differences can be recorded at the
level of 9-day old seedlings germinated in drought stress conditions and in normal conditions.
The correlation analyses show significant correlation of seedling properties and
subsequent seedling development. The results of morpho-physiological properties of
one-year old seedlings in nursery conditions also point to the wide modification variability
of the tested planting stock.