Zbornik - Društvo genetičara Srbije

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132 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-15 Subotica, 30. novembar - 4. decembar 2004. UTICAJ KOLHICINA NA REGENERACIONU SPOSOBNOST KALUSA U KULTURI ANTERA PŠENICE Branka Ljevnaiæ 1 , Ankica Kondiæ-Špika 2 , B. Kobiljski 2 i S. Denèiæ 2 1 Poljoprivredni fakultet, Novi Sad 2 Nauèni institut za ratarstvo i povrtarstvo, Novi Sad Cilj istraivanja je bio da se ispita uticaj kolhicina na regeneracionu sposobnost kalusa u kulturi antera pšenice. Kao materijal korišæena je F2 generacija 14 razlièitih kombinacija ukrštanja pšenice (Triticum aestivum L.). Od ukupnog broja izolovanih antera, polovina je bila izloena delovanju kolhicina u indukcionoj podlozi (300mg/l) u trajanju od tri dana. Druga polovina antera gajena je na modifikovanoj Potato-2 indukcionoj hranljivoj podlozi bez kolhicina. Rezultati su pokazali da je kolhicin kod veæine kombinacija znaèajno poveæao regeneracionu sposobnost kalusa. Kombinacija Banks/F53-70 pokazala se kao najosetljivija na prisustvo kolhicina u podlozi. Kod ove kombinacije, ukupna regeneraciona sposobnost kalusa iznosila je 40,9% na podlozi bez kolhicina i 15,0% na podlozi sa kolhicinom. Pozitivan uticaj kolhicina na regeneraciju zelenih biljaka bio je najviše izraen kod kombinacije Prima/Bezostja-1. Kod ove kombinacije svaki kalus je dao zelenu biljku (100,0%) na podlozi sa kolhicinom, dok je na podlozi bez kolhicina 37,5% kalusa regenerisalo zelene biljke. Na podlozi bez kolhicina, u proseku za sve ispitivane kombinacije, 18,1% kalusa je regenerisalo biljke (13,9% zelenih i 4,2% albino) dok je na podlozi sa kolhicinom 30,3% kalusa regenerisalo biljke (18,2% zelenih i 12,1% albino). Kolhicin je kod veæine kombinacija ukrštanja smanjio broj formiranih kalusa, ali je njihova regeneraciona sposobnost znaèajno poveæana. EFFECT OF COLCHICINE ON REGENERATION ABILITY OF CALLI IN WHEAT ANTHER CULTURE The aim of this study was to investigate effect of colchicine on regeneration ability of calli in wheat anther culture. F2 generations of 14 different wheat (Triticum aestivum L.) combinations were used as a material. A half of isolated anthers were exposed to colchicine effect in induction medium (300mg/l) for three days. The other half were grown on a modified Potato-2 induction medium without colchicine. The results have shown that colchicine in most of the combinations significantly increased regeneration ability of calli. The combination Banks/F53-70 was the most sensitive one to the presence of colchicine. In this combination, whole regeneration ability of calli was 40.9% and 15.0%, on media without and with colchicine, respectively. The positive effect of colchicine was the most expressed at Prima/Bezostja-1 combination. In this combination, every callus regenereted green plants (100.0%) on medium with colchicine, while 37.5% of calli regenereted green plants on medium without colchicine. In average 18.1% of calli regenereted plants (13.9% green and 4.2% albino) on medium without colchicine, while on medium with colchicine 30.3% of calli regenereted plants (18.2% green and 12.1% albino). Colchicine has decreased a number of formed calli in most of the studied combinations but their regeneration ability was significantly increased.
IV-Pos-16 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 133 Subotica, 30. novembar - 4. decembar 2004. PRIMENA SSR MARKERA RADI UTVRÐIVANJA PRISUSTVA QTL-OVA ZA UKORENJAVANJE I SADRAJ ABA U LISTU KOD KOMPOZITNIH POPULACIJA KUKURUZA V. Laziæ-Janèiæ, D. Ignjatoviæ-Miciæ i K. Markoviæ Institut za kukuruz «Zemun Polje», Beograd-Zemun Tolerantnost kukuruza na sušu je kompleksna osobina, èija je ekspresija uslovljena aktivnošæu veæeg broja gena. Sadraj ABA u listu i ukorenjavanje su neke od fiziološko-morfoloških osobina koje utièu na ispoljavanje ove tolerantnosti, a èiji su QTL-ovi mapirani kod razlièitih genotipova kukuruza. Tako je, na primer, u segregirajuæoj populaciji ukrštanja Os420xIABO78, QTL sa major efektom za sadraj ABA u listu identifikovan na hromozomu 2 (bin 2.04), a za ukorenjavanje na hromozomu 1 (bin 1.06). Cilj ovog rada je bio da se utvrdi da li se QTL-ovi sa major efektom detektovani u jednom genetipu mogu detektovati u nekom drugom genetièkom okruenju primenom molekularnih markera datog hromozomskog regiona. Za realizaciju navedenog cilja analizirano je 10 tolerantnih i 10 netolerantnih populacija kukuruza, korišæenjem 12 SSR prajmer kombinacije – dve na bin 1.05/ 1.06, a 10 na bin 2.04/2.05. Elektroforeza je raðena na denaturišuæem poliakrilamidnom gelu, a nakon razdvajanja amplifikovanih fragmenata DNK je bojena srebro-nitratom. Analizom elektroforegrama, na osnovu prisustva/odsustva traka, izraèunata je razlika u frekvenciji alela izmeðu tolerantnih i netolerantnih populacija. Rezultati i diskusija æe biti prikazani na posteru. SSR MARKER APPLICATION FOR DETERMINING PRESENCE OF QTLs FOR ROOTING AND LEAF ABA CONTENT IN COMPOSITE MAIZE POPULATIONS Maize drought tolerance is a complex trait with its expression determined by many genes. Leaf ABA content and rooting are some of physiological-morphological traits influencing drought tolerance, whose QTLs were mapped in different maize genotypes. For example, in a segregating population, derived from the cross Os420xIABO78, a QTL with a major effect for leaf ABA content was identified on chromosome 2 (bin 2.04) and for rooting on chromosome 1 (bin 1.06). The objective of this study was to determine if the QTLs with major effects detected in one genotype could also be detected in some different genetic background using molecular markers within a chromosome region harboring the QTLs in question. In order to fulfill the objective 10 drought tolerant and 10 drought non-tolerant maize populations were analyzed with 12 SSR primer combinations – two on bin 1.05/1.06 and 10 on bin 2.04/2.05. Electrophoresis was run on a polyacrilamide denaturating gel and then silver stained. Allele frequency differences between tolerant and non-tolerant populations were calculated based on presence/absence of the bands on the gel. Results and discussion will be presented on the poster.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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