Zbornik - Društvo genetičara Srbije

132 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-15
Subotica, 30. novembar - 4. decembar 2004.
UTICAJ KOLHICINA NA REGENERACIONU SPOSOBNOST
KALUSA U KULTURI ANTERA PŠENICE
Branka Ljevnaiæ 1 , Ankica Kondiæ-Špika 2 , B. Kobiljski 2 i S. Denèiæ 2
1
Poljoprivredni fakultet, Novi Sad
2
Nauèni institut za ratarstvo i povrtarstvo, Novi Sad
Cilj istraivanja je bio da se ispita uticaj kolhicina na regeneracionu sposobnost kalusa u
kulturi antera pšenice. Kao materijal korišæena je F2 generacija 14 razlièitih kombinacija
ukrštanja pšenice (Triticum aestivum L.). Od ukupnog broja izolovanih antera, polovina
je bila izloena delovanju kolhicina u indukcionoj podlozi (300mg/l) u trajanju od tri
dana. Druga polovina antera gajena je na modifikovanoj Potato-2 indukcionoj hranljivoj
podlozi bez kolhicina. Rezultati su pokazali da je kolhicin kod veæine kombinacija
znaèajno poveæao regeneracionu sposobnost kalusa. Kombinacija Banks/F53-70
pokazala se kao najosetljivija na prisustvo kolhicina u podlozi. Kod ove kombinacije,
ukupna regeneraciona sposobnost kalusa iznosila je 40,9% na podlozi bez kolhicina i
15,0% na podlozi sa kolhicinom. Pozitivan uticaj kolhicina na regeneraciju zelenih
biljaka bio je najviše izraen kod kombinacije Prima/Bezostja-1. Kod ove kombinacije
svaki kalus je dao zelenu biljku (100,0%) na podlozi sa kolhicinom, dok je na podlozi bez
kolhicina 37,5% kalusa regenerisalo zelene biljke. Na podlozi bez kolhicina, u proseku
za sve ispitivane kombinacije, 18,1% kalusa je regenerisalo biljke (13,9% zelenih i 4,2%
albino) dok je na podlozi sa kolhicinom 30,3% kalusa regenerisalo biljke (18,2% zelenih
i 12,1% albino). Kolhicin je kod veæine kombinacija ukrštanja smanjio broj formiranih
kalusa, ali je njihova regeneraciona sposobnost znaèajno poveæana.
EFFECT OF COLCHICINE ON REGENERATION ABILITY OF CALLI IN
WHEAT ANTHER CULTURE
The aim of this study was to investigate effect of colchicine on regeneration ability of
calli in wheat anther culture. F2 generations of 14 different wheat (Triticum aestivum L.)
combinations were used as a material. A half of isolated anthers were exposed to
colchicine effect in induction medium (300mg/l) for three days. The other half were
grown on a modified Potato-2 induction medium without colchicine. The results have
shown that colchicine in most of the combinations significantly increased regeneration
ability of calli. The combination Banks/F53-70 was the most sensitive one to the presence
of colchicine. In this combination, whole regeneration ability of calli was 40.9%
and 15.0%, on media without and with colchicine, respectively. The positive effect of
colchicine was the most expressed at Prima/Bezostja-1 combination. In this combination,
every callus regenereted green plants (100.0%) on medium with colchicine, while 37.5%
of calli regenereted green plants on medium without colchicine. In average 18.1% of calli
regenereted plants (13.9% green and 4.2% albino) on medium without colchicine, while
on medium with colchicine 30.3% of calli regenereted plants (18.2% green and 12.1% albino).
Colchicine has decreased a number of formed calli in most of the studied combinations
but their regeneration ability was significantly increased.