Zbornik - Društvo genetičara Srbije

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128 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-11 Subotica, 30. novembar - 4. decembar 2004. EFEKAT GENA I KOMBINACIONE SPOSOBNOSTI ZA VISINU BILJKE I PREÈNIK GLAVE KOD SUNCOKRETA Sandra Gvozdenoviæ, J. Joksimoviæ i D. Škoriæ Nauèni institut za ratarstvo i povrtarstvo, Novi Sad Visina biljke i preènik glave su znaèajni parametri koji utièu na visinu prinosa kod suncokreta. Metodom linija x tester ispitano je šest restorer linija, tri tester A linije i njihovih 18 F1 hibrida. Utvrðene su znaèajne razlike izmeðu restorer linija i testera i njihovih F1 hibrida za visinu biljke i preènik glave. U nasleðivanju ispitivanih svojstava ispoljile su se superdominacija i dominacija boljeg roditelja, ali i intermedijarnost. Najveæu srednju vrednost za visinu biljke su imali linija RHA-SELEUS (172,7 cm) i F1 hibrid HA-48A x RUS-RF-OL-168 (237,4 cm), a najmanju linija RHA-BRE-1 (105,35 cm), odnosno hibrid L-19A x RHA-BRE-1 (147,9 cm). Kod preènika glave najveæu srednju vrednost su imali linija RUS-RF-OL-168 (16,45 cm) i F1 hibridi HA-48A x RHA-TR-20 i L-19A x RHA-TR-20 (24,55 cm), a najmanju linija RHA-BRE-1 (13,10 cm) i hibrid HA-26A x RHA-M-72 (20,25 cm). Na osnovu dobijenih rezultata moe se zakljuèiti da najbolje OKS imaju linije RHA-BRE-1 i RHA-TR-20, a najbolje PKS, hibridi L-19A x RHA-BRE-1 i HA-26A x RHA-SELEUS. Najveæi proseèni doprinos u ekspresiji visine biljke dale su A-tester linije 83,17%, a za preènik glave najveæi uticaj su imale restorer linije 58,13%. Analiziranjem komponenti genetske varijanse, neaditivna komponenta je imala najveæi uticaj na nasleðivanje visine biljke, a aditivna na preènika glave. GENE EFFECT AND COMBINING ABILITIES FOR PLANT HEIGHT AND HEAD DIAMETER IN SUNFLOWER Plant height and head diameter are important parameters which effect on a yield in sunflower. Six restorer Rf lines, three tester A lines and their 18 F1 hybrids were studied, using line x tester method. Significant differences were found between restorer lines and testers and their F1 hybrids for plant height and head diameter. Regarding the inheritance of examined characteristic, super dominance and dominance of better parent occurred, and the intermediary too. Line RHA-SELEUS (172.7 cm) and F1 hybrid HA-48A x RUS-RF-OL-168 (237.4 cm) had the highest mean value for plant height, and the lowest value had line RHA-BRE-1 (105.35 cm) and hybrid L-19A x RHA-BRE-1 (147.9 cm). For head diameter, highest mean value had line RUS-RF-OL-168 (16.45 cm) and F1 hybrids HA-48A x RHA-TR-20 and L-19A x RHA-TR-20 (24.55 cm), and the lowest line RHA-BRE-1 (13.10 cm) and hybrid HA-26A x RHA-M-72 (20.25 cm). Based on the results, the following conclusion is that lines RHA-BRE-1 and RHA-SELEUS have the best GCA, and best SCA have hybrids L-19A x RHA-BRE-1 and HA-26A x SELEUS. The largest average contribution in the expression of plant height had the tester A-lines (82.88%) and for head diameter Rf lines had the greatest influence (47.15%). Analyzing components of genetic variance, the non additive component played the main role in the inheritance of plant height and the additive of head diameter.
IV-Pos-12 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 129 Subotica, 30. novembar - 4. decembar 2004. VARIJABILNOST AKTIVNOSTI PEROKSIDAZA I SADRAJA TEŠKIH METALA U ÈETINAMA LINIJA POLUSRODNIKA CRNOG BORA (Pinus nigra Arn.) V. Isajev 1 , Ksenija Radotiæ 2 i M. Duèiæ 1 1 Šumarski fakultet, Beograd 2 Centar za multidisciplinarne studije, Univerzitet u Beogradu, Beograd Ekstrakcija enzima je raðena najpre homogenizacijom u 5 mL 0.1 M TRIS-HCl puferu pH 7.6 koji sadri 1 mM DTT i 1 mM EDTA na ledenom kupatilu po proceduri Mocquot et al. (1996) i Weckx i Clijsters (1996)Koncentracija proteina je uraðena po metodi Bradford (Bradford, 1976). Za odreðivanje koncentracije metala u èetinama crnog bora, uzorci su pripremani prema proceduri za «suvo spaljivanje» Uzorak je oèitavan na apsorberu Varian AA-10. Detekcioni limit za Cd je od 0.02 do 3 g/mL, za Cu je 0.03-10 g/mL i za Ni 0.1-20 g/mL. U cilju utvrðivanja koliki je uticaj genetièke raznolikosti linija polusrodnika na jedan od kljuènih enzima ukljuèenih u odgovor na stres, i zaduenog za proces lignifikacije u æelijskom zidu, praæena je aktivnost enzima peroksidaza. Takoðe je praæena aktivnost ovog enzima u zavisnosti od kiselosti medijuma. Izoelektrofokusiranjem utvrðeni su izoenzimi koji su prisutni u èetinama analiziranih osam linija crnog bora. Rezultati ovog rada pokazuju prisustvo izraenog varijabiliteta u koncentraciji ukupnih proteina, sadraju teških metala i aktivnosti enzima peroksidaza kod analiziranih linija polusrodnika crnog bora. Ovo govori da proteini imaju razlièit nivo ekspresije u pojedinim linijama crnog bora. Takoðe enzim peroksidaza pokazuje razlièitu aktivnost koja zavisi od genetske konstitucije stabala iz analiziranih linija polusrodnika, jer se, na osnovu velièine plantae i šeme sadnje biljaka, moe smatrati da je uticaj spoljašnjih faktora sveden na minimum. VARIABILITY OF PEROXIDASE ACTIVITY AND HEAVY METAL CONTENT IN THE NEEDLES OF AUSTRIAN PINE (Pinus nigra Arn.) HALF-SIB LINES Enzyme extraction was performed first by homogenisation in 5 mL 0.1 M TRIS-HCl buffer pH 7.6 containing 1 mM DTT and 1 mM EDTA in ice bath, by procedure Mocquot et al. (1996) and Weckx and Clijsters (1996). The concentration of proteins was determined by Bradford (Bradford, 1976) method. For the measurement of metal concentration in Austrian pine needles, the samples were prepared by the procedure for «dry burning». The sample was read off on the absorber Varian AA-10. Detection limit for Cd is from 0.02 to 3 g/mL, for Cu 0.03-10 g/mL and for Ni 0.1-20 g/mL. The enzyme activity of peroxidases was monitored in order to determine the effect of genetic variability of half-sib lines on one of the key enzymes included in the response to stress, and responsible for the process of lignification in the cell wall. Also, the activity of this enzyme was monitored depending on the medium acidity. The isoenzymes present in the needles of the analysed eight Austrian pine lines were determined by isoelectric focusing. The results of this study show the pronounced variability of the concentration of total proteins, heavy metal concentration and enzyme activity of peroxidases in the study Austrian pine half-sib lines. This infers that proteins have different levels of expression in individual Austrian pine lines. Also the enzyme of peroxidases shows different activity depending on the genetic constitution of the trees of the analysed half-sib lines, because based on he seed orchard size and the planting pattern, it can be assumed that the effect of external factors is reduced to a minimum.
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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Uvodno predavanje Opening lecture
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Stanojeviæ B. 223 Stanojeviæ J. 2
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Zbornik - Društvo genetičara Srbije

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