Zbornik - Društvo genetičara Srbije

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126 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-9 Subotica, 30. novembar - 4. decembar 2004. GENETIÈKA KARAKTERIZACIJA RANIH HIBRIDA KUKURUZA PRIMENOM PROTEINSKIH I RAPD MARKERA I. Eriæ, S. Mladenoviæ-Driniæ i K. Konstantinov Institut za kukuruz «Zemun Polje», Zemun-Beograd Poznavanje genetièke varijabilnosti germplazme kukuruza je vano zbog planiranja programa selekcije, konzervacije germplazme per se. Proizvoðaèi kukuruza daju prednost uniformnosti useva koja se u veæini sluèajeva postie gajenjem inbred linija i dvolinijskih hibrida. Genetièka uniformnost ukljuèuje rizik od velikih gubitaka u prinosima zbog izuzetne osetljivosti ovakvih useva na stresne faktore sredine. Ovaj rizik se moe smanjiti gajenjem nesrodnih dvolinijskih hibrida. Zbog toga je postojanje informacije o genetièkoj varijabilnosti hibrida koji se gaje na poljima je takoðe vrlo vano. U ovom radu ispitivali smo informativnost proteinskih i RAPD markera za genetièku karakterizaciju hibrida i utvrðivanje njihove genetièke srodnosti. Kao materijal je izabrano 11 ranih hibrida kukuruza FAO 100-200 sa najvišim prinosom koji su pogodni za gajenje u brdsko-planinskim podruèjima. Proteini klice rastvorljivi u solima izolovani su i razdvojeni PAA elektroforezom. Amplifikacija genomske DNK ovih genotipova uraðena je sa 15 RAPD prajmera. Dobijeni rezultati kao prisustvo/odsustvo proteinske frakcije ili RAPD fragmenta prevedeni su u binarni kod (1,0). Za izraèunavanje koeficijenata sliènosti i UPGMA klaster metodu korišæen je NTSYS-pc programski paket. RAPD markeri su detektovali visok nivo polimorfizma meðu ispitivanim hibridima i informativniji su za procenu genetièke srodnosti od proteinskih markera. GENETIC CHARACTERIZATION OF EARLY MATURING MAIZE HYBRIDS OBTAINED BY PROTEIN AND RAPD MARKERS Knowledge on genetic diversity of maize germplasm is important for planning breeding programmes, germplasm conservation per se etc. The maize farmers prefer uniform crop fields, which in the majority of cases is attained by using inbred lines and single cross hybrids. This genetic uniformity may cause losts in yield bacause of genetic sucseptibility of material to environmental stress factors. This risk may be reduced by the use of unrelated single cross hybrids, so information on genetic variability of maize hybrids is very important. In our study we examined usefulness of protein and RAPD markers for characterization of maize hybrids and evaluation of their genetic relatedness. 11 early maturing ZP maize hybrids from groups FAO 100-200 were chosen for the analysis on the basis of their superior yield. The salt solubile proteins from embryo tissue were isolated and separated by gel electrophoresis (SDS-PAGE). The amplification of genomic DNA from embryo tissue was performed with 15 RAPD primers. Presense/absense of protein fractions and DNA fragments were transformed to binary data (1,0). The computing of binary data was performed using NTSYS-pc software including coefficients of similarities and UPGMA clustering. RAPD markers detected high level of polymorphism among hybrids and were more informative in assessing genetic relatedness between them.
IV-Pos-10 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE 127 Subotica, 30. novembar - 4. decembar 2004. PRINOS ZRNA ZP HIBRIDA KUKURUZA RAZLIÈITIH FAO GRUPE ZRENJA U ZAVISNOSTI OD GUSTINE SETVE Branka J. Kresoviæ, . V. Videnoviæ, M. M. Tolimir i Lj. M. Prijiæ Institut za kukuruz «Zemun Polje», Zemun-Beograd U periodu 1998-2000 u Zemun Polju na èernozemu obavljena su prouèavanja uticaja navodnjavanja i gustine setve na genotipove kukuruza razlièitih FAO grupa zrenja (ZP 360, ZP 580 i ZP 704). Ogled je postavljen po split-plot metodi, u èetiri ponavljanja, sa varijantama sedam gustina setve (40816, 50125, 59523, 69686, 79365, 89286 i 98522 bilj/ha). Rezultati prinosa zrna kukuruza obraðeni su metodom analize varijanse, uz korišæenje LSD testa. Regresionom analizom utvrðene su gustine pri kojima se postiu maksimalni prinosi zrna kukuruza. U uslovima prirodnog vodnog reima, dobijeni rezultati pokazuju da su, u godinama prouèavanja, izmeðu ispitivanih hibrida i gustina ostvarene statistièki veoma znaèajne razlike prinosa zrna kukuruza. U relativno povoljnijim godinama za gajenje kukuruza ispitivani hibridi ostvarili su najbolje rezultate prinosa u sledeæim gustinama: ZP 360 – 80-90.000 bilj./ha; ZP 580 – 70-80.000 bilj./ha; ZP 704 – 70-80.000 bilj./ha. U uslovima izrazitog deficita vode tokom vegetacije najpovoljnije gustine bile su: ZP 360 – 70.000 bilj./ha; ZP 580 – 50-60.000 bilj./ha; ZP 704 – 60-70.000 bilj./ha. U uslovima navodnjavanja svi hibridi ostvarili su veoma znaèajno nie prinose u gustinama setve sa oko 50.000 bilj.·ha -1 . Najbolje rezultate prinosa u navodnjavanju hibridi ZP 360 i ZP 580 ostvarili su u gustinama 80, 90 i 100.000 bilj.·ha -1 , a ZP 704 u gustinama 70, 80, 90 i 100.000 bilj.·ha -1 . YIELD OF ZP MAIZE HYBRIDS OF DIFFERENT MATURITY GROUPS IN VARIOUS SOWING RATES Investigations were carried out on chernozem at Zemun Polje location in the period 1998 – 2000. The aim was to determine effects of irrigation and sowing rate on yield of different FAO maturity group maize (ZP 360, ZP 580 and ZP 704). The trial was set up using split-plot method in four replications. Seven sowing rate variants: 40816, 50125, 59523, 69686, 79365, 89286 and 98522 plants per hectare were applied. Analysis of variance method with LSD test was used to analyze the maize grain yield data. Crop density variants with maximal yields were determined by regression analysis. Obtained results indicated that very significant maize yield differences between investigated hybrids and densities were achieved under dryland farming. Investigated hybrids, grown in relatively favourable years, showed the best yield results in the next densities: 80-90,000 plants per ha (ZP 360), 70-80,000 plants per ha (ZP 580 and ZP 704). The most favourable densities under extreme water deficiency during growing period were: 70,000 plants per ha (ZP 360), 50,000-60,000 (ZP 580) and 60,000-70,000 (ZP 704). All hybrids produced very significantly lower yields, when grown under irrigation and crop density around 50,000 plants per ha. The best yield results under irrigation with hybrids ZP 360 and ZP 580 were attained in crop densities of 80, 90 and 100,000 plants ha -1 .
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DRUŠTVO GENETIČARA SRBIJE SERBIAN
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ZAVRŠNO PREDAVANJE CLOSING LECTURE
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A Aksentijeviæ I. 194 Aleksiæ J.
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Zbornik - Društvo genetičara Srbije

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