Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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126 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-9<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
GENETIÈKA KARAKTERIZACIJA RANIH HIBRIDA KUKURUZA<br />
PRIMENOM PROTEINSKIH I RAPD MARKERA<br />
I. Eriæ, S. Mladenoviæ-Driniæ i K. Konstantinov<br />
Institut za kukuruz «Zemun Polje», Zemun-Beograd<br />
Poznavanje genetièke varijabilnosti germplazme kukuruza je vano zbog planiranja<br />
programa selekcije, konzervacije germplazme per se. Proizvoðaèi kukuruza daju<br />
prednost uniformnosti useva koja se u veæini sluèajeva postie gajenjem inbred linija i<br />
dvolinijskih hibrida. Genetièka uniformnost ukljuèuje rizik od velikih gubitaka u<br />
prinosima zbog izuzetne osetljivosti ovakvih useva na stresne faktore sredine. Ovaj rizik<br />
se moe smanjiti gajenjem nesrodnih dvolinijskih hibrida. Zbog toga je postojanje<br />
informacije o genetièkoj varijabilnosti hibrida koji se gaje na poljima je takoðe vrlo<br />
vano. U ovom radu ispitivali smo informativnost proteinskih i RAPD markera za<br />
genetièku karakterizaciju hibrida i utvrðivanje njihove genetièke srodnosti. Kao materijal<br />
je izabrano 11 ranih hibrida kukuruza FAO 100-200 sa najvišim prinosom koji su<br />
pogodni za gajenje u brdsko-planinskim podruèjima. Proteini klice rastvorljivi u solima<br />
izolovani su i razdvojeni PAA elektroforezom. Amplifikacija genomske DNK ovih<br />
genotipova uraðena je sa 15 RAPD prajmera. Dobijeni rezultati kao prisustvo/odsustvo<br />
proteinske frakcije ili RAPD fragmenta prevedeni su u binarni kod (1,0). Za<br />
izraèunavanje koeficijenata sliènosti i UPGMA klaster metodu korišæen je NTSYS-pc<br />
programski paket. RAPD markeri su detektovali visok nivo polimorfizma meðu<br />
ispitivanim hibridima i informativniji su za procenu genetièke srodnosti od proteinskih<br />
markera.<br />
GENETIC CHARACTERIZATION OF EARLY MATURING MAIZE HYBRIDS<br />
OBTAINED BY PROTEIN AND RAPD MARKERS<br />
Knowledge on genetic diversity of maize germplasm is important for planning breeding<br />
programmes, germplasm conservation per se etc. The maize farmers prefer uniform crop<br />
fields, which in the majority of cases is attained by using inbred lines and single cross hybrids.<br />
This genetic uniformity may cause losts in yield bacause of genetic sucseptibility<br />
of material to environmental stress factors. This risk may be reduced by the use of unrelated<br />
single cross hybrids, so information on genetic variability of maize hybrids is very<br />
important. In our study we examined usefulness of protein and RAPD markers for characterization<br />
of maize hybrids and evaluation of their genetic relatedness. 11 early maturing<br />
ZP maize hybrids from groups FAO 100-200 were chosen for the analysis on the basis<br />
of their superior yield. The salt solubile proteins from embryo tissue were isolated and<br />
separated by gel electrophoresis (SDS-PAGE). The amplification of genomic DNA from<br />
embryo tissue was performed with 15 RAPD primers. Presense/absense of protein fractions<br />
and DNA fragments were transformed to binary data (1,0). The computing of binary<br />
data was performed using NTSYS-pc software including coefficients of similarities<br />
and UPGMA clustering. RAPD markers detected high level of polymorphism among hybrids<br />
and were more informative in assessing genetic relatedness between them.