Zbornik - Društvo genetičara Srbije

126 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Pos-9
Subotica, 30. novembar - 4. decembar 2004.
GENETIÈKA KARAKTERIZACIJA RANIH HIBRIDA KUKURUZA
PRIMENOM PROTEINSKIH I RAPD MARKERA
I. Eriæ, S. Mladenoviæ-Driniæ i K. Konstantinov
Institut za kukuruz «Zemun Polje», Zemun-Beograd
Poznavanje genetièke varijabilnosti germplazme kukuruza je vano zbog planiranja
programa selekcije, konzervacije germplazme per se. Proizvoðaèi kukuruza daju
prednost uniformnosti useva koja se u veæini sluèajeva postie gajenjem inbred linija i
dvolinijskih hibrida. Genetièka uniformnost ukljuèuje rizik od velikih gubitaka u
prinosima zbog izuzetne osetljivosti ovakvih useva na stresne faktore sredine. Ovaj rizik
se moe smanjiti gajenjem nesrodnih dvolinijskih hibrida. Zbog toga je postojanje
informacije o genetièkoj varijabilnosti hibrida koji se gaje na poljima je takoðe vrlo
vano. U ovom radu ispitivali smo informativnost proteinskih i RAPD markera za
genetièku karakterizaciju hibrida i utvrðivanje njihove genetièke srodnosti. Kao materijal
je izabrano 11 ranih hibrida kukuruza FAO 100-200 sa najvišim prinosom koji su
pogodni za gajenje u brdsko-planinskim podruèjima. Proteini klice rastvorljivi u solima
izolovani su i razdvojeni PAA elektroforezom. Amplifikacija genomske DNK ovih
genotipova uraðena je sa 15 RAPD prajmera. Dobijeni rezultati kao prisustvo/odsustvo
proteinske frakcije ili RAPD fragmenta prevedeni su u binarni kod (1,0). Za
izraèunavanje koeficijenata sliènosti i UPGMA klaster metodu korišæen je NTSYS-pc
programski paket. RAPD markeri su detektovali visok nivo polimorfizma meðu
ispitivanim hibridima i informativniji su za procenu genetièke srodnosti od proteinskih
markera.
GENETIC CHARACTERIZATION OF EARLY MATURING MAIZE HYBRIDS
OBTAINED BY PROTEIN AND RAPD MARKERS
Knowledge on genetic diversity of maize germplasm is important for planning breeding
programmes, germplasm conservation per se etc. The maize farmers prefer uniform crop
fields, which in the majority of cases is attained by using inbred lines and single cross hybrids.
This genetic uniformity may cause losts in yield bacause of genetic sucseptibility
of material to environmental stress factors. This risk may be reduced by the use of unrelated
single cross hybrids, so information on genetic variability of maize hybrids is very
important. In our study we examined usefulness of protein and RAPD markers for characterization
of maize hybrids and evaluation of their genetic relatedness. 11 early maturing
ZP maize hybrids from groups FAO 100-200 were chosen for the analysis on the basis
of their superior yield. The salt solubile proteins from embryo tissue were isolated and
separated by gel electrophoresis (SDS-PAGE). The amplification of genomic DNA from
embryo tissue was performed with 15 RAPD primers. Presense/absense of protein fractions
and DNA fragments were transformed to binary data (1,0). The computing of binary
data was performed using NTSYS-pc software including coefficients of similarities
and UPGMA clustering. RAPD markers detected high level of polymorphism among hybrids
and were more informative in assessing genetic relatedness between them.